##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546817_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4535386
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.554141367460232	31.0	31.0	33.0	30.0	34.0
2	31.929369848564157	31.0	31.0	34.0	30.0	34.0
3	31.912443174627253	33.0	31.0	34.0	30.0	34.0
4	35.6713448425338	37.0	35.0	37.0	33.0	37.0
5	35.61394708190218	37.0	35.0	37.0	33.0	37.0
6	35.6766187045601	37.0	35.0	37.0	33.0	37.0
7	36.051299271991404	37.0	35.0	37.0	35.0	37.0
8	35.93990743015038	37.0	35.0	37.0	35.0	37.0
9	37.688663103868116	39.0	38.0	39.0	35.0	39.0
10	37.353449739448855	39.0	37.0	39.0	34.0	39.0
11	37.050141487405924	39.0	37.0	39.0	33.0	39.0
12	36.30880370491067	38.0	35.0	39.0	32.0	39.0
13	36.05525306997023	38.0	35.0	39.0	32.0	39.0
14	37.07939324238334	39.0	35.0	41.0	32.0	41.0
15	37.27660997321948	39.0	35.0	41.0	33.0	41.0
16	37.392283038312506	39.0	35.0	41.0	33.0	41.0
17	37.30051995574357	39.0	35.0	41.0	33.0	41.0
18	37.1544556516248	39.0	35.0	41.0	32.0	41.0
19	37.04201538744442	38.0	35.0	41.0	32.0	41.0
20	36.859745785694976	38.0	35.0	41.0	32.0	41.0
21	36.71248026077604	38.0	35.0	41.0	32.0	41.0
22	36.64419544444508	38.0	35.0	41.0	32.0	41.0
23	36.55932130142837	38.0	35.0	40.0	32.0	41.0
24	36.510433070084886	38.0	35.0	40.0	32.0	41.0
25	36.39777540434265	37.0	35.0	40.0	31.0	41.0
26	36.33315532569885	37.0	35.0	40.0	31.0	41.0
27	36.23215377919322	37.0	35.0	40.0	31.0	41.0
28	36.21240772009262	37.0	35.0	40.0	31.0	41.0
29	36.132995295218535	37.0	35.0	40.0	31.0	41.0
30	36.11660308516188	37.0	35.0	40.0	31.0	41.0
31	35.926947563007865	37.0	35.0	40.0	30.0	41.0
32	35.62200659436705	37.0	35.0	40.0	30.0	41.0
33	35.43791443550781	37.0	35.0	40.0	29.0	41.0
34	35.14925829907311	37.0	34.0	40.0	27.0	41.0
35	34.95422572632186	37.0	34.0	40.0	25.0	41.0
36	34.80873888132124	37.0	34.0	40.0	24.0	41.0
37	34.510051845642245	36.0	34.0	40.0	23.0	41.0
38	34.619384546320866	36.0	34.0	40.0	24.0	41.0
39	34.533314694713965	36.0	34.0	40.0	23.0	41.0
40	34.38434567642093	36.0	34.0	40.0	23.0	41.0
41	34.32873894305799	36.0	34.0	40.0	23.0	41.0
42	34.32180149605789	36.0	34.0	40.0	23.0	41.0
43	34.22156438283313	36.0	34.0	40.0	23.0	41.0
44	34.08796053963213	35.0	34.0	40.0	23.0	41.0
45	34.07026392020437	35.0	34.0	40.0	23.0	41.0
46	33.89235403557713	35.0	33.0	40.0	23.0	41.0
47	33.77445403764972	35.0	33.0	40.0	22.0	41.0
48	33.63080055369047	35.0	33.0	40.0	21.0	41.0
49	33.69118416822736	35.0	33.0	40.0	22.0	41.0
50	33.540209366964575	35.0	33.0	40.0	21.0	41.0
51	32.95938250900805	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	7.0
12	12.0
13	22.0
14	53.0
15	116.0
16	318.0
17	867.0
18	2035.0
19	4120.0
20	7450.0
21	12353.0
22	19372.0
23	29155.0
24	43250.0
25	65322.0
26	86903.0
27	96483.0
28	95413.0
29	97819.0
30	109845.0
31	129535.0
32	161602.0
33	213418.0
34	385190.0
35	696094.0
36	310811.0
37	369393.0
38	551240.0
39	1046758.0
40	423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.376286825421253	24.060091026430825	29.2987631041768	17.26485904397112
2	33.557871369713624	25.603730310937152	28.605282990246035	12.23311532910319
3	27.186418090984983	23.709889301594174	35.80202434809298	13.301668259327872
4	25.27756182163988	26.428930194695667	34.27456891210583	14.018939071558629
5	22.959192448007734	30.04075066598521	33.10258487370204	13.897472012305018
6	20.38000734667347	38.693222583480214	30.16572349078998	10.761046579056337
7	82.17803291715413	4.693801145040356	11.079564120892908	2.048601816912607
8	83.12225243893243	4.256043476784556	10.384253953246759	2.2374501310362556
9	79.02903964513716	5.8553560821504504	12.0346096230839	3.080994649628499
10	48.81771915334219	22.691762068322298	18.00825773153597	10.482261046799545
11	42.72366232995383	21.50086012524623	22.913992326121747	12.86148521867819
12	37.454408511205	21.245843242449485	26.92143513253337	14.378313113812144
13	21.749063916500162	36.25451064143162	27.709548867505436	14.286876574562783
14	15.659615300660187	39.690910542123646	29.71416324872899	14.935310908487171
15	14.886847558289414	24.11463985645323	46.88802673024964	14.11048585500771
16	15.517929455177576	21.027273092080804	45.87574243956303	17.57905501317859
17	16.67743385017284	20.755080162967385	31.163808328552413	31.403677658307366
18	20.658418048651207	24.82664099593728	34.73430927378618	19.780631681625334
19	26.926241779641245	26.28803810745105	27.441809804060778	19.34391030884692
20	29.051771999119808	24.840708155821797	27.158570406135222	18.948949438923172
21	21.954779593181264	27.86186225384124	30.22743819379431	19.955919959183188
22	21.688870583451994	25.901213259466783	28.64620563718281	23.763710519898417
23	19.9713761959842	29.72648855025791	27.614408123145417	22.68772713061248
24	20.45153378345305	25.51152206228974	36.21898996028122	17.817954193975993
25	17.70599018473841	26.66562449149863	35.1629828199849	20.46540250377807
26	17.021616241704677	34.35923645749226	29.234270247339477	19.384877053463587
27	19.133740766497052	31.9749851501063	31.15871504652526	17.732559036871393
28	16.055391977661877	29.067228235920822	38.289618568298266	16.58776121811903
29	16.44170970232743	25.85438152342491	37.7107924220783	19.993116352169363
30	18.516196857334748	28.966597330414658	33.422645834334716	19.094559977915882
31	26.025392326033554	26.915967020227168	27.402915650398885	19.6557250033404
32	26.57641488508365	27.8720267690556	28.16525429147596	17.386304054384787
33	24.389059718401036	28.75536944374746	27.491838621894587	19.363732215956922
34	19.132197347700945	28.950744214494645	29.810891509565007	22.106166928239404
35	19.97106751222498	26.801048466437035	31.257956875114928	21.969927146223057
36	26.216842403270636	26.691377536553667	27.425008588023157	19.666771472152536
37	20.678394297640818	30.52088620461412	29.96157328174493	18.839146216000138
38	20.15332322320526	31.60515995771914	25.432631312968727	22.80888550610687
39	20.8918491171424	30.16667159090759	28.124971060897575	20.81650823105244
40	22.326853767242742	27.832912126994263	26.70319571476386	23.137038390999134
41	17.773018658169338	26.50266151546969	28.447258954364635	27.27706087199634
42	22.1073575655964	27.84084970937424	25.456510206628497	24.59528251840086
43	22.342993518082032	28.79651257908368	26.32924738930711	22.531246513527183
44	20.182714326851123	32.340047793065466	26.739951130951145	20.737286749132267
45	17.902621739362427	35.3592615931698	24.1463681371332	22.591748530334574
46	22.02873140235473	32.47809557995725	25.932566709867693	19.56060630782033
47	22.134587882927715	28.436014045992998	27.626270399035498	21.80312767204379
48	22.786153152124207	25.411177791702844	29.72972972972973	22.072939326443215
49	20.415505978983926	25.69450538498818	30.378340454373674	23.51164818165422
50	18.728372844119555	31.76854186170703	27.996029444902813	21.5070558492706
51	18.908533915305114	32.59283774302782	25.215935313995324	23.282693027671737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4793.0
1	6412.0
2	8031.0
3	44292.0
4	80553.0
5	55836.5
6	31120.0
7	32463.0
8	33806.0
9	36247.5
10	38689.0
11	39811.0
12	40933.0
13	40980.0
14	41027.0
15	39199.5
16	37372.0
17	35240.0
18	33108.0
19	30717.5
20	28327.0
21	27809.5
22	27292.0
23	26558.5
24	25825.0
25	29519.0
26	37548.0
27	41883.0
28	52197.5
29	62512.0
30	68243.5
31	73975.0
32	82659.0
33	91343.0
34	93713.0
35	96083.0
36	104627.5
37	113172.0
38	138394.0
39	163616.0
40	223917.5
41	284219.0
42	338261.5
43	392304.0
44	407071.0
45	421838.0
46	420427.0
47	419016.0
48	397500.5
49	375985.0
50	350504.0
51	325023.0
52	293897.5
53	262772.0
54	238129.0
55	213486.0
56	197020.5
57	180555.0
58	170652.0
59	160749.0
60	147667.0
61	134585.0
62	114781.0
63	94977.0
64	77844.0
65	60711.0
66	47891.0
67	35071.0
68	29751.0
69	24431.0
70	21432.5
71	18434.0
72	15064.0
73	11694.0
74	8914.0
75	5034.0
76	3934.0
77	2843.0
78	1752.0
79	1157.5
80	563.0
81	381.5
82	200.0
83	183.5
84	167.0
85	118.5
86	70.0
87	46.5
88	23.0
89	16.5
90	10.0
91	9.5
92	9.0
93	4.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4535386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.86860136617706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49117047596103	16.79738150159831
2	8.310776028098271	3.4686854394792626
3	2.796204300044279	1.750586186280426
4	1.4036246491627369	1.1716673308446914
5	0.8560735279437758	0.8932528597397746
6	0.5853534721692573	0.7329304961404707
7	0.4520444563663676	0.6603474891789961
8	0.3460652320530788	0.5777517899527409
9	0.28505771708223954	0.5353880277727563
>10	2.543275096281893	11.68538054671661
>50	0.7509010668521902	11.378348278379944
>100	1.1637609307261634	45.78903518609898
>500	0.011956607435321277	1.6627234018473256
>1k	0.0035229289764785276	1.4046508358988183
>5k	0.0	0.0
>10k+	2.1351084705930473E-4	1.491870630070807
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54990	1.2124657085416763	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11975	0.2640348583339985	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4097679888768014E-5	0.0	0.0	0.037527125585341575	0.0
2	4.4097679888768014E-5	0.0	0.0	0.1791909222280088	0.0
3	4.4097679888768014E-5	0.0	0.0	0.29097854074603574	0.0
4	4.4097679888768014E-5	0.0	0.0	0.4197878637011271	0.0
5	4.4097679888768014E-5	0.0	0.0	0.7841449437820728	0.0
6	4.4097679888768014E-5	0.0	0.0	1.2421434471068173	0.0
7	4.4097679888768014E-5	0.0	0.0	1.5634611916163255	0.0
8	4.4097679888768014E-5	0.0	0.0	2.1807846123791887	0.0
9	4.4097679888768014E-5	0.0	0.0	2.5095989624697874	0.0
10	4.4097679888768014E-5	0.0	0.0	3.0194563373437235	0.0
11	4.4097679888768014E-5	0.0	0.0	3.464446025101281	0.0
12	4.4097679888768014E-5	0.0	0.0	3.8370273224814824	0.0
13	4.4097679888768014E-5	0.0	0.0	3.9982704889947627	0.0
14	4.4097679888768014E-5	0.0	0.0	4.064505204187692	0.0
15	4.4097679888768014E-5	0.0	0.0	4.149878312452347	0.0
16	4.4097679888768014E-5	0.0	0.0	4.3162588586726685	0.0
17	4.4097679888768014E-5	0.0	0.0	4.517278132445617	0.0
18	4.4097679888768014E-5	0.0	0.0	4.804398126201386	0.0
19	4.4097679888768014E-5	0.0	0.0	4.950581935032652	0.0
20	4.4097679888768014E-5	0.0	0.0	5.098970627858357	0.0
21	4.4097679888768014E-5	0.0	0.0	5.296197501160871	0.0
22	4.4097679888768014E-5	0.0	0.0	5.498208972731318	0.0
23	4.4097679888768014E-5	0.0	0.0	5.724011142601754	0.0
24	4.4097679888768014E-5	0.0	0.0	5.899806543478328	0.0
25	4.4097679888768014E-5	0.0	0.0	6.038692186288003	0.0
26	4.4097679888768014E-5	0.0	0.0	6.183310527483218	0.0
27	4.4097679888768014E-5	0.0	0.0	6.317235181305406	0.0
28	4.4097679888768014E-5	0.0	0.0	6.472392867994036	0.0
29	4.4097679888768014E-5	0.0	0.0	6.633239155388318	0.0
30	4.4097679888768014E-5	0.0	0.0	6.8511037428787755	0.0
31	4.4097679888768014E-5	0.0	0.0	7.039863861642647	0.0
32	4.4097679888768014E-5	0.0	0.0	7.206597189302079	0.0
33	4.4097679888768014E-5	0.0	0.0	7.365613422980977	0.0
34	4.4097679888768014E-5	0.0	0.0	7.531376601682855	0.0
35	4.4097679888768014E-5	0.0	0.0	7.727875863267206	0.0
36	4.4097679888768014E-5	0.0	0.0	7.91509256323497	0.0
37	4.4097679888768014E-5	0.0	0.0	8.100655600206906	0.0
38	4.4097679888768014E-5	0.0	0.0	8.282801066987462	0.0
39	4.4097679888768014E-5	0.0	0.0	8.466578147923903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAT	30	2.166922E-6	45.000004	12
CGTTTTT	32695	0.0	43.947086	1
CGGTCTA	410	0.0	41.707317	31
CTCACGA	425	0.0	40.764706	24
GACCGAT	1695	0.0	40.35398	9
CGACGGT	425	0.0	40.235294	28
TCTAGCG	80	0.0	39.375	1
TACGCAT	40	3.4612276E-7	39.375	25
GGGACCG	2130	0.0	38.97887	7
ACGGTCT	445	0.0	38.932587	30
TACCGGT	110	0.0	38.863636	20
TATCGCT	35	6.252363E-6	38.571426	1
TACGTTG	175	0.0	38.571426	1
GTTTTTT	38900	0.0	38.510284	2
AGGGCGA	3270	0.0	38.394493	6
TATTCCG	65	9.094947E-12	38.076927	1
ATTAGCG	405	0.0	37.777775	1
GCGATCG	155	0.0	37.741936	9
TCACGAC	455	0.0	37.582417	25
GGCGATA	1830	0.0	37.5	8
>>END_MODULE