FastQCFastQC Report
Thu 26 May 2016
SRR1546816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546816_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3472927
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310270.8933962620003242No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC104630.3012732487610595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG302.1667747E-645.00000437
CTTACGT600.045.00000424
AGCCGTA351.212884E-745.038
AACCGGT207.0352596E-445.036
CGAACTT551.8189894E-1245.025
ACGTTAT453.8562575E-1045.022
TAGCGGA1000.045.03
GTTCGAC253.892441E-545.024
CTTGCGT1000.045.036
CGTACTC253.892441E-545.045
CGCGTCA453.8562575E-1045.039
TAGTCGA207.0352596E-445.018
TCGTTAG1400.043.3928571
CGTTTTT217400.043.1784741
GCGTAAG2350.043.0851061
TATAGCG3000.042.751
CGACGGT2450.042.244928
CGGTCTA2400.042.18750431
TAATGCG1850.041.3513531
CGCTCCG603.6379788E-1241.2500043
Produced by FastQC (version 0.11.5)