##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546816_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3472927
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606101711898926	31.0	31.0	33.0	30.0	34.0
2	31.97294472357179	31.0	31.0	34.0	30.0	34.0
3	31.96881420196854	33.0	31.0	34.0	30.0	34.0
4	35.70907997778243	37.0	35.0	37.0	33.0	37.0
5	35.65277387057085	37.0	35.0	37.0	33.0	37.0
6	35.71426292576838	37.0	35.0	37.0	33.0	37.0
7	36.102322335021725	37.0	35.0	37.0	35.0	37.0
8	36.01339360142036	37.0	35.0	37.0	35.0	37.0
9	37.812856705597326	39.0	38.0	39.0	35.0	39.0
10	37.42514743327458	39.0	37.0	39.0	35.0	39.0
11	37.150031371232394	39.0	37.0	39.0	34.0	39.0
12	36.22783605874814	37.0	35.0	39.0	33.0	39.0
13	35.86713368867241	37.0	35.0	39.0	32.0	39.0
14	36.83310965073553	38.0	35.0	41.0	32.0	41.0
15	37.0788784215735	38.0	35.0	41.0	32.0	41.0
16	37.21069259445995	38.0	35.0	41.0	33.0	41.0
17	37.12033250338979	38.0	35.0	41.0	33.0	41.0
18	36.993811560104774	38.0	35.0	40.0	32.0	41.0
19	36.914569468347594	38.0	35.0	40.0	32.0	41.0
20	36.75362367248145	38.0	35.0	40.0	32.0	41.0
21	36.581733218118316	37.0	35.0	40.0	32.0	41.0
22	36.531917889434474	37.0	35.0	40.0	32.0	41.0
23	36.463646946797326	37.0	35.0	40.0	32.0	41.0
24	36.402349084792164	37.0	35.0	40.0	32.0	41.0
25	36.30347312223954	37.0	35.0	40.0	32.0	41.0
26	36.24209204512505	37.0	35.0	40.0	32.0	41.0
27	36.14527112144885	37.0	35.0	40.0	31.0	41.0
28	36.16704641358716	36.0	35.0	40.0	31.0	41.0
29	36.12274142243704	36.0	35.0	40.0	31.0	41.0
30	36.097434815070976	36.0	35.0	40.0	31.0	41.0
31	35.876070530707956	36.0	35.0	40.0	31.0	41.0
32	35.58830462028139	36.0	35.0	40.0	30.0	41.0
33	35.41615329086963	36.0	35.0	40.0	29.0	41.0
34	35.18053733925303	36.0	34.0	40.0	28.0	41.0
35	35.00936817848461	36.0	34.0	40.0	27.0	41.0
36	34.846276066269176	36.0	34.0	40.0	25.0	41.0
37	34.53302128147237	36.0	34.0	40.0	24.0	41.0
38	34.65449201782819	36.0	34.0	40.0	25.0	41.0
39	34.601767039733346	36.0	34.0	40.0	24.0	41.0
40	34.483324584709095	36.0	34.0	40.0	23.0	41.0
41	34.46380905789267	36.0	34.0	40.0	23.0	41.0
42	34.47514070983928	36.0	34.0	40.0	24.0	41.0
43	34.358435118273434	35.0	34.0	40.0	23.0	41.0
44	34.23171607119873	35.0	34.0	40.0	23.0	41.0
45	34.207467649046464	35.0	34.0	40.0	23.0	41.0
46	34.04053180501634	35.0	34.0	40.0	23.0	41.0
47	33.939157661534495	35.0	33.0	40.0	23.0	41.0
48	33.82321857038745	35.0	33.0	40.0	23.0	41.0
49	33.9078039935766	35.0	34.0	40.0	23.0	41.0
50	33.75938999005738	35.0	33.0	40.0	23.0	41.0
51	33.15441412963762	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	10.0
13	8.0
14	20.0
15	68.0
16	193.0
17	568.0
18	1449.0
19	2873.0
20	5545.0
21	8800.0
22	14323.0
23	21329.0
24	31724.0
25	47903.0
26	63838.0
27	69618.0
28	68689.0
29	70926.0
30	81032.0
31	98573.0
32	124208.0
33	164667.0
34	311688.0
35	605638.0
36	216134.0
37	261110.0
38	404182.0
39	797594.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.461884168598996	23.80706533710614	30.211922104898836	15.519128389396034
2	31.39968101834562	25.876472497118424	30.025393565715607	12.69845291882035
3	27.04868256660736	24.901991893293467	35.372007531399305	12.677318008699867
4	24.630520595451618	27.162016362566792	34.70634424507051	13.501118796911078
5	22.99547327081738	32.496421606328035	32.41977156444694	12.08833355840765
6	19.62278504558259	40.106572928253314	29.773387116976547	10.497254909187554
7	86.05470256069304	4.158106404194503	8.606342718980272	1.1808483161321848
8	87.8055311845023	3.07630998290491	7.910647128488447	1.2075117041043477
9	83.78578069737716	5.166679288104818	9.109347821016682	1.938192193501332
10	47.276461612927655	26.376223859585878	15.006563627741096	11.340750899745373
11	45.40665553868538	18.78352179587996	23.99886320674175	11.810959458692913
12	41.78993684577879	22.750319831081967	23.129855594430865	12.329887728708378
13	21.795592017914572	41.39963206828131	23.637640526276538	13.167135387527582
14	15.290099676728017	43.395412572737634	27.74406142138893	13.570426329145416
15	13.70777445077308	25.587753500145556	48.54357721887042	12.160894830210943
16	16.473769820096994	21.13600429839153	46.279435185363816	16.11079069614766
17	17.394146205779737	20.460550999200386	29.005245431303333	33.140057363716544
18	21.353227407313774	25.562414643325354	34.89200895958942	18.19234898977145
19	26.971571818238623	26.162283284388067	27.295765214759772	19.57037968261354
20	29.51795416373566	25.421496046418486	27.247477416024008	17.81307237382185
21	22.127790189658462	27.96332891535008	30.314083768532996	19.59479712645846
22	23.196139740340065	24.467257733894204	28.170272510766853	24.166330014998877
23	18.9862902387525	30.760652325833508	26.852479191183694	23.4005782442303
24	19.99319306164512	25.34118914679174	38.44903736819115	16.216580423371983
25	16.257900036482194	27.55298340564026	36.23116754253689	19.957949015340663
26	16.297031293776115	36.90641928263968	28.312688403758557	18.48386101982564
27	17.46690903667137	34.48065565443789	31.468671814869705	16.583763494021035
28	14.24631154066872	30.42258014637221	39.85027615034811	15.48083216261096
29	14.801203710875582	26.04969813647105	39.25081062746208	19.898287525191286
30	18.075300747755424	31.38395940945491	33.60263547146254	16.938104371327125
31	28.15299601748036	27.92483112947666	26.092946957998254	17.82922589504473
32	27.57083578203631	29.172741033715944	27.202356974390767	16.054066209856988
33	25.189789477291058	30.93664220411198	26.007860228562247	17.86570809003472
34	18.068246179663436	30.42903003719917	29.09508319639313	22.407640586744265
35	19.37904251946557	28.833805029590316	30.812309040760145	20.974843410183976
36	28.926551004383334	25.58763832352364	28.05984692451065	17.425963747582372
37	20.432937404097466	31.619927513592998	30.17667805859438	17.770457023715156
38	18.99058056791865	33.11811621724269	25.197506310959028	22.693796903879637
39	19.511322869729195	32.53264465391872	28.693779051503242	19.262253424848836
40	22.268536021632475	29.23375584917276	25.36111470238217	23.1365934268126
41	17.58559854554962	26.736870656941537	28.30125712403399	27.376273673474856
42	23.11876984457203	27.838765398754422	25.034445008489957	24.008019748183592
43	22.764716908820716	29.14627920483212	26.19493585669955	21.894068029647613
44	19.950145799206258	34.04690049632485	26.155487863695377	19.847465840773502
45	17.516233425004327	38.976575090694396	21.992918365401863	21.514273118899418
46	22.874307464568073	34.19766669440504	24.734093172704178	18.193932668322716
47	22.23749592202773	30.33855880068887	25.857410766192324	21.566534511091078
48	24.33805835826667	26.354973772843483	28.7615316993418	20.54543616954805
49	21.33382014652194	25.230130089115033	30.406311448527422	23.029738315835605
50	19.95509839394839	33.53160028989956	26.79713682435594	19.71616449179611
51	18.544155981395523	35.417819032764	23.72831332187518	22.309711663965295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3509.0
1	4494.5
2	5480.0
3	24989.5
4	44499.0
5	31650.0
6	18801.0
7	20189.0
8	21577.0
9	24368.0
10	27159.0
11	28696.0
12	30233.0
13	30264.0
14	30295.0
15	29307.5
16	28320.0
17	26451.5
18	24583.0
19	22895.0
20	21207.0
21	20527.5
22	19848.0
23	18710.0
24	17572.0
25	18959.0
26	24160.5
27	27975.0
28	33584.5
29	39194.0
30	42552.5
31	45911.0
32	55174.0
33	64437.0
34	74552.0
35	84667.0
36	93160.5
37	101654.0
38	127776.0
39	153898.0
40	203189.5
41	252481.0
42	303697.5
43	354914.0
44	364319.0
45	373724.0
46	347745.0
47	321766.0
48	308427.0
49	295088.0
50	283909.5
51	272731.0
52	252875.5
53	233020.0
54	200741.5
55	168463.0
56	144230.5
57	119998.0
58	105395.0
59	90792.0
60	77201.5
61	63611.0
62	52310.0
63	41009.0
64	32424.0
65	23839.0
66	18726.0
67	13613.0
68	10508.0
69	7403.0
70	5716.5
71	4030.0
72	3221.5
73	2413.0
74	2032.5
75	1177.5
76	703.0
77	473.0
78	243.0
79	175.5
80	108.0
81	92.0
82	76.0
83	55.0
84	34.0
85	29.0
86	24.0
87	22.0
88	20.0
89	11.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3472927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.82835995877177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01478803394066	15.25375591086631
2	8.067429619995572	3.0379293765466797
3	2.6040636109167585	1.4709074106563917
4	1.3355380608024465	1.0058396538971395
5	0.7914123841946443	0.7450498622723272
6	0.5923279007428127	0.6691537757285587
7	0.43397257843470194	0.571969434330342
8	0.3392722232203909	0.511035163424503
9	0.26258932530119006	0.4449713704291639
>10	2.4681283478394764	10.262074884408936
>50	0.7154455413472972	9.729774252808612
>100	1.353051744027846	52.16958484093451
>500	0.019503938618162565	2.3206425980002963
>1k	0.0021671042909069517	0.5980848331352868
>5k	0.0	0.0
>10k+	3.0958632727242166E-4	1.2092266325609402
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31027	0.8933962620003242	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10463	0.3012732487610595	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02628906395095549	0.0
2	0.0	0.0	0.0	0.10495469671547948	0.0
3	0.0	0.0	0.0	0.1554596454230106	0.0
4	0.0	0.0	0.0	0.22686915100720517	0.0
5	0.0	0.0	0.0	0.4148373979643108	0.0
6	0.0	0.0	0.0	0.6402380470421636	0.0
7	0.0	0.0	0.0	0.7973389593273915	0.0
8	2.87941554774978E-5	0.0	0.0	1.1653858546407685	0.0
9	2.87941554774978E-5	0.0	0.0	1.3237537097670062	0.0
10	2.87941554774978E-5	0.0	0.0	1.5847727291705238	0.0
11	2.87941554774978E-5	0.0	0.0	1.8812085598113637	0.0
12	2.87941554774978E-5	0.0	0.0	2.089447892224628	0.0
13	2.87941554774978E-5	0.0	0.0	2.177039713187176	0.0
14	2.87941554774978E-5	0.0	0.0	2.212830848445706	0.0
15	2.87941554774978E-5	0.0	0.0	2.2615217653581547	0.0
16	2.87941554774978E-5	0.0	0.0	2.3779941242646334	0.0
17	2.87941554774978E-5	0.0	0.0	2.516868336132605	0.0
18	2.87941554774978E-5	0.0	0.0	2.689287739131862	0.0
19	2.87941554774978E-5	0.0	0.0	2.7787511802004476	0.0
20	2.87941554774978E-5	0.0	0.0	2.883273964583765	0.0
21	2.87941554774978E-5	0.0	0.0	3.0199598206354468	0.0
22	2.87941554774978E-5	0.0	0.0	3.163959392178413	0.0
23	2.87941554774978E-5	0.0	0.0	3.3235078076792286	0.0
24	2.87941554774978E-5	0.0	0.0	3.442398875645817	0.0
25	2.87941554774978E-5	0.0	0.0	3.5539474339656434	0.0
26	2.87941554774978E-5	0.0	0.0	3.660572191698818	0.0
27	2.87941554774978E-5	0.0	0.0	3.7678016266970196	0.0
28	2.87941554774978E-5	0.0	0.0	3.886807871285518	0.0
29	5.75883109549956E-5	0.0	0.0	4.0144523625172654	0.0
30	5.75883109549956E-5	0.0	0.0	4.171582068957971	0.0
31	5.75883109549956E-5	0.0	0.0	4.308642249030861	0.0
32	5.75883109549956E-5	0.0	0.0	4.4365458876619055	0.0
33	5.75883109549956E-5	0.0	0.0	4.565226968490844	0.0
34	5.75883109549956E-5	0.0	0.0	4.705224152422438	0.0
35	5.75883109549956E-5	0.0	0.0	4.875311228828018	0.0
36	5.75883109549956E-5	0.0	0.0	5.028006635325188	0.0
37	5.75883109549956E-5	0.0	0.0	5.180039776246376	0.0
38	5.75883109549956E-5	0.0	0.0	5.340480810566994	0.0
39	5.75883109549956E-5	0.0	0.0	5.504233172767524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATCG	30	2.1667747E-6	45.000004	37
CTTACGT	60	0.0	45.000004	24
AGCCGTA	35	1.212884E-7	45.0	38
AACCGGT	20	7.0352596E-4	45.0	36
CGAACTT	55	1.8189894E-12	45.0	25
ACGTTAT	45	3.8562575E-10	45.0	22
TAGCGGA	100	0.0	45.0	3
GTTCGAC	25	3.892441E-5	45.0	24
CTTGCGT	100	0.0	45.0	36
CGTACTC	25	3.892441E-5	45.0	45
CGCGTCA	45	3.8562575E-10	45.0	39
TAGTCGA	20	7.0352596E-4	45.0	18
TCGTTAG	140	0.0	43.392857	1
CGTTTTT	21740	0.0	43.178474	1
GCGTAAG	235	0.0	43.085106	1
TATAGCG	300	0.0	42.75	1
CGACGGT	245	0.0	42.2449	28
CGGTCTA	240	0.0	42.187504	31
TAATGCG	185	0.0	41.351353	1
CGCTCCG	60	3.6379788E-12	41.250004	3
>>END_MODULE