FastQCFastQC Report
Thu 26 May 2016
SRR1546815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546815_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5718354
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610191.0670727975218044No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC114440.20012751921269653No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT207.0356415E-445.00000416
GTATCGA453.8562575E-1045.013
CGTTTTT302400.042.961311
CGTAATC700.041.78571312
CACGACG10350.041.08695626
GTCGAAC451.9306754E-840.038
TAACGGG18850.039.6286473
CGGTCTA10650.039.50704631
TCACGAC11150.038.9461925
TACGGGA14450.038.6159174
TACCGAT700.038.57142621
TAACGCC4400.038.35227212
CGACGGT11000.038.2528
ACGGGTA5000.038.255
CGCATCG3900.038.07692321
TACGCGG3900.038.0769232
TTAGACG2550.037.9411771
CGGGTAT6350.037.9133876
TCTAGCG2200.037.8409081
CTAACGG4350.037.758622
Produced by FastQC (version 0.11.5)