##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546815_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5718354
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.600643471880193	31.0	31.0	33.0	30.0	34.0
2	31.977212673437148	31.0	31.0	34.0	30.0	34.0
3	31.993676676889887	33.0	31.0	34.0	30.0	34.0
4	35.73499821801868	37.0	35.0	37.0	33.0	37.0
5	35.66832291250245	37.0	35.0	37.0	33.0	37.0
6	35.72802872994571	37.0	35.0	37.0	33.0	37.0
7	36.07167604523959	37.0	35.0	37.0	35.0	37.0
8	35.9559053182087	37.0	35.0	37.0	35.0	37.0
9	37.71153429815643	39.0	38.0	39.0	35.0	39.0
10	37.40176333959038	39.0	37.0	39.0	35.0	39.0
11	37.101951890351664	39.0	37.0	39.0	33.0	39.0
12	36.38235355838411	38.0	35.0	39.0	33.0	39.0
13	36.13926052846676	38.0	35.0	39.0	32.0	39.0
14	37.113925265906936	39.0	35.0	41.0	32.0	41.0
15	37.323413170992914	39.0	35.0	41.0	33.0	41.0
16	37.434541478194596	39.0	35.0	41.0	33.0	41.0
17	37.350502259916055	39.0	35.0	41.0	33.0	41.0
18	37.22255932388936	39.0	35.0	41.0	32.0	41.0
19	37.13969386994929	38.0	35.0	41.0	32.0	41.0
20	36.99611339207051	38.0	35.0	41.0	32.0	41.0
21	36.85607379326289	38.0	35.0	41.0	32.0	41.0
22	36.807664583199994	38.0	35.0	41.0	32.0	41.0
23	36.709297465669316	38.0	35.0	41.0	32.0	41.0
24	36.680235606260126	38.0	35.0	41.0	32.0	41.0
25	36.58079860043642	38.0	35.0	41.0	32.0	41.0
26	36.51719428352984	38.0	35.0	40.0	32.0	41.0
27	36.42381636393969	38.0	35.0	40.0	31.0	41.0
28	36.39905679151728	38.0	35.0	40.0	31.0	41.0
29	36.29821466107205	38.0	35.0	40.0	31.0	41.0
30	36.28238038428541	38.0	35.0	40.0	31.0	41.0
31	36.11695760703167	38.0	35.0	40.0	31.0	41.0
32	35.876231167220496	38.0	35.0	40.0	30.0	41.0
33	35.72092983400468	38.0	35.0	40.0	30.0	41.0
34	35.4883566145083	37.0	35.0	40.0	29.0	41.0
35	35.32745192060513	37.0	35.0	40.0	28.0	41.0
36	35.21322709297116	37.0	34.0	40.0	27.0	41.0
37	34.93123703079593	37.0	34.0	40.0	25.0	41.0
38	35.02895570998228	37.0	34.0	40.0	27.0	41.0
39	34.93552252973495	37.0	34.0	40.0	26.0	41.0
40	34.789704344991584	37.0	34.0	40.0	25.0	41.0
41	34.73516784725115	36.0	34.0	40.0	24.0	41.0
42	34.737707564099736	36.0	34.0	40.0	25.0	41.0
43	34.62882063614809	36.0	34.0	40.0	24.0	41.0
44	34.49863194898392	36.0	34.0	40.0	24.0	41.0
45	34.48482710234448	36.0	34.0	40.0	24.0	41.0
46	34.31833058953678	35.0	34.0	40.0	23.0	41.0
47	34.19123282678897	35.0	34.0	40.0	23.0	41.0
48	34.05119322798134	35.0	33.0	40.0	23.0	41.0
49	34.11179685622821	35.0	34.0	40.0	24.0	41.0
50	33.97519181218931	35.0	33.0	40.0	24.0	41.0
51	33.40087042530071	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	14.0
12	19.0
13	34.0
14	74.0
15	182.0
16	495.0
17	1070.0
18	2457.0
19	4928.0
20	8616.0
21	14025.0
22	21600.0
23	31616.0
24	46107.0
25	68154.0
26	92045.0
27	106837.0
28	111310.0
29	118353.0
30	134760.0
31	160554.0
32	199821.0
33	265263.0
34	479485.0
35	838029.0
36	402704.0
37	494740.0
38	734085.0
39	1380572.0
40	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.36743545432829	23.24322698454835	29.41717144478988	15.972166116333478
2	32.959851733558295	24.408982025247123	29.654512469847095	12.97665377134749
3	28.27874944433311	24.380249981026008	33.5878471322342	13.75315344240668
4	25.910043344640783	26.173685644505397	32.79263578295433	15.123635227899495
5	23.256097821156228	30.590935783269103	31.41989810354518	14.733068292029488
6	21.442394087529383	38.326605872948754	29.179288305690765	11.051711733831098
7	84.11235121155494	4.58014666458215	9.264554100708002	2.042948023154915
8	85.03826450758383	3.591085826445862	8.971445279533237	2.3992043864370762
9	80.60228520304969	5.4468121420954345	10.555222709192192	3.395679945662686
10	48.92512775529462	23.889374459853308	16.027724061854162	11.157773722997911
11	40.749838152727165	21.182861361853426	23.570209189567485	14.497091295851918
12	36.76440807966768	20.602799337012016	27.539690617265038	15.093101966055267
13	23.011464487857868	32.92774459223756	28.105587726817895	15.955203193086682
14	16.91700094117993	35.670894106940565	31.000161934710587	16.411943017168927
15	15.326735630567818	23.55856248144134	45.45890303398495	15.655798854005889
16	18.28165937260967	20.231118954860086	43.3093858827208	18.177835789809446
17	18.92200098140129	20.214715633204943	30.32390089875513	30.53938248663864
18	22.4340255954773	23.96929256216037	33.68005898200776	19.916622860354572
19	27.409705660055323	24.935951849081047	27.666143089427482	19.988199401436148
20	29.389401215804405	24.356029724637544	26.21277031817198	20.04179874138607
21	23.810645510928495	26.47553824054964	29.94700922678099	19.76680702174087
22	23.163326369791026	23.943778227091222	29.016986356563446	23.875909046554305
23	20.880624039714927	27.646784371866453	28.64985273734365	22.822738851074977
24	21.02970540123959	23.91314003994856	36.11243725029965	18.944717308512203
25	20.065354470884454	24.471430065364963	33.98425490971702	21.478960554033556
26	18.125373140592554	30.981590156887805	29.459263977011567	21.433772725508074
27	18.62717838035211	30.258934651474885	31.412658257953254	19.70122871021976
28	16.75875610359205	27.767291077117644	36.860309802436156	18.61364301685415
29	18.267739283017455	24.925214493541322	36.56176235329257	20.245283870148647
30	20.299267936192827	27.45122110313562	32.55466520610651	19.694845754565037
31	26.776341583609547	25.423294885206477	27.631832516839637	20.16853101434434
32	28.241675838886504	26.468962921847787	27.757270711117222	17.532090528148483
33	26.36337309652393	26.561192259171087	28.030915889432517	19.04451875487247
34	20.69144372663882	27.80401493156947	29.82893329094351	21.6756080508482
35	20.535682121113872	26.61421450997962	31.590244325552426	21.259859043354083
36	27.431949823323286	25.817411793673493	27.8598177027865	18.89082068021672
37	21.312129329523845	30.2546327142391	29.29419899502549	19.139038961211565
38	21.905009728323922	30.605065723458186	25.963485296643057	21.526439251574843
39	20.93245713714121	28.704239017031824	28.47203933159787	21.891264514229096
40	23.43307182451454	26.06697661599824	27.711086092256622	22.788865467230604
41	19.15766320168356	25.46605194431824	28.742694138907805	26.633590715090392
42	22.314777993807308	26.62210139491189	26.731398580780414	24.331722030500387
43	22.295384301146797	26.696510919051182	27.679433627229095	23.328671152572923
44	21.009734619437694	29.348812612860275	28.343400915718053	21.298051851983978
45	18.85909826499024	32.93096230138953	25.534410776247853	22.675528657372386
46	22.22093980190803	30.235990986217367	27.560081100260668	19.982988111613935
47	22.098824242080852	27.67787723530233	28.367516246808083	21.85578227580874
48	22.437348229927704	25.542909725420987	30.151578583627387	21.868163461023922
49	21.53675690592083	25.035858220739744	31.217742028562768	22.20964284477666
50	19.942119707873978	29.718377001493785	28.48050330567153	21.858999984960708
51	19.043277138841002	31.15265336843434	27.045282611045067	22.758786881679587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4642.0
1	7972.0
2	11302.0
3	52427.5
4	93553.0
5	62925.5
6	32298.0
7	33529.5
8	34761.0
9	37435.5
10	40110.0
11	41506.0
12	42902.0
13	42035.0
14	41168.0
15	39788.5
16	38409.0
17	35711.0
18	33013.0
19	30308.0
20	27603.0
21	27526.5
22	27450.0
23	30609.5
24	33769.0
25	37328.5
26	46527.5
27	52167.0
28	60087.0
29	68007.0
30	76222.0
31	84437.0
32	99534.0
33	114631.0
34	124547.0
35	134463.0
36	151140.5
37	167818.0
38	192129.5
39	216441.0
40	278723.5
41	341006.0
42	398920.0
43	456834.0
44	473325.0
45	489816.0
46	486012.5
47	482209.0
48	447078.5
49	411948.0
50	396248.0
51	380548.0
52	358024.0
53	335500.0
54	310320.5
55	285141.0
56	273153.0
57	261165.0
58	251729.0
59	242293.0
60	225248.0
61	208203.0
62	185778.0
63	163353.0
64	142047.0
65	120741.0
66	96975.0
67	73209.0
68	61498.5
69	49788.0
70	41211.0
71	32634.0
72	25771.0
73	18908.0
74	15529.5
75	9592.0
76	7033.0
77	4961.0
78	2889.0
79	2264.5
80	1640.0
81	1187.5
82	735.0
83	568.5
84	402.0
85	321.5
86	241.0
87	154.0
88	67.0
89	51.5
90	36.0
91	26.0
92	16.0
93	14.0
94	12.0
95	7.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5718354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.868824418330078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.27464410534294	14.522823451806385
2	8.24036182046561	2.944911570268215
3	2.6944998494277623	1.4444263411392455
4	1.298286314635709	0.927954008037853
5	0.7998817315933717	0.7146473108635893
6	0.5298946204581582	0.5681156359910691
7	0.3817393585260249	0.4774863499747246
8	0.3059012292340186	0.4372876283627214
9	0.23842832473309072	0.3834390483910966
>10	1.9955464476583176	7.849744543600642
>50	0.6937957664383098	9.088287238299642
>100	1.5253864666681156	56.2690128036324
>500	0.01807769662910443	2.083948998061092
>1k	0.0033586977343681634	1.0088156700307649
>5k	0.0	0.0
>10k+	1.975704549628331E-4	1.2790994015404686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61019	1.0670727975218044	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11444	0.20012751921269653	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.246264921689003E-5	0.0	0.0	0.03581450186539693	0.0
2	5.246264921689003E-5	0.0	0.0	0.13418196914706573	0.0
3	5.246264921689003E-5	0.0	0.0	0.20558363473125307	0.0
4	5.246264921689003E-5	0.0	0.0	0.31813350485122116	0.0
5	5.246264921689003E-5	0.0	0.0	0.5807440392812337	0.0
6	5.246264921689003E-5	0.0	0.0	0.9169246954630651	0.0
7	5.246264921689003E-5	0.0	0.0	1.1647057877144367	0.0
8	5.246264921689003E-5	0.0	0.0	1.652660888080731	0.0
9	5.246264921689003E-5	0.0	0.0	1.8955804415046706	0.0
10	5.246264921689003E-5	0.0	0.0	2.252448869027696	0.0
11	5.246264921689003E-5	0.0	0.0	2.5874753469267553	0.0
12	5.246264921689003E-5	0.0	0.0	2.8544752563412477	0.0
13	5.246264921689003E-5	0.0	0.0	2.9624259008798686	0.0
14	5.246264921689003E-5	0.0	0.0	3.0082957438451694	0.0
15	5.246264921689003E-5	0.0	0.0	3.0640110773135065	0.0
16	5.246264921689003E-5	0.0	0.0	3.1815623866588183	0.0
17	5.246264921689003E-5	0.0	0.0	3.3173881854813465	0.0
18	5.246264921689003E-5	0.0	0.0	3.5182676693328183	3.497509947792669E-5
19	5.246264921689003E-5	0.0	0.0	3.6180166530438655	3.497509947792669E-5
20	5.246264921689003E-5	0.0	0.0	3.721280634252444	3.497509947792669E-5
21	5.246264921689003E-5	0.0	0.0	3.859484739839471	3.497509947792669E-5
22	5.246264921689003E-5	0.0	0.0	4.003162448494794	3.497509947792669E-5
23	6.995019895585339E-5	0.0	0.0	4.157857313485664	3.497509947792669E-5
24	6.995019895585339E-5	0.0	0.0	4.2768076268100925	3.497509947792669E-5
25	8.743774869481672E-5	0.0	0.0	4.373583727065515	3.497509947792669E-5
26	8.743774869481672E-5	0.0	0.0	4.474784177404897	3.497509947792669E-5
27	8.743774869481672E-5	0.0	0.0	4.578520322456427	3.497509947792669E-5
28	8.743774869481672E-5	0.0	0.0	4.682955969497516	3.497509947792669E-5
29	8.743774869481672E-5	0.0	0.0	4.804581877932006	3.497509947792669E-5
30	8.743774869481672E-5	0.0	0.0	4.9591018674254865	3.497509947792669E-5
31	8.743774869481672E-5	0.0	0.0	5.095417317640706	3.497509947792669E-5
32	8.743774869481672E-5	0.0	0.0	5.219753796284735	3.497509947792669E-5
33	8.743774869481672E-5	0.0	0.0	5.342131669358	3.497509947792669E-5
34	8.743774869481672E-5	0.0	0.0	5.4743375453845635	3.497509947792669E-5
35	8.743774869481672E-5	0.0	0.0	5.6371641210040515	3.497509947792669E-5
36	8.743774869481672E-5	0.0	0.0	5.771853229093547	3.497509947792669E-5
37	8.743774869481672E-5	0.0	0.0	5.907486664868946	3.497509947792669E-5
38	8.743774869481672E-5	0.0	0.0	6.063003444697547	3.497509947792669E-5
39	8.743774869481672E-5	0.0	0.0	6.286756643607584	3.497509947792669E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	20	7.0356415E-4	45.000004	16
GTATCGA	45	3.8562575E-10	45.0	13
CGTTTTT	30240	0.0	42.96131	1
CGTAATC	70	0.0	41.785713	12
CACGACG	1035	0.0	41.086956	26
GTCGAAC	45	1.9306754E-8	40.0	38
TAACGGG	1885	0.0	39.628647	3
CGGTCTA	1065	0.0	39.507046	31
TCACGAC	1115	0.0	38.94619	25
TACGGGA	1445	0.0	38.615917	4
TACCGAT	70	0.0	38.571426	21
TAACGCC	440	0.0	38.352272	12
CGACGGT	1100	0.0	38.25	28
ACGGGTA	500	0.0	38.25	5
CGCATCG	390	0.0	38.076923	21
TACGCGG	390	0.0	38.076923	2
TTAGACG	255	0.0	37.941177	1
CGGGTAT	635	0.0	37.913387	6
TCTAGCG	220	0.0	37.840908	1
CTAACGG	435	0.0	37.75862	2
>>END_MODULE