FastQCFastQC Report
Thu 26 May 2016
SRR1546814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546814_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1958618
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81190.4145269776954975No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29140.14877837332241406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG351.2125201E-745.0000041
TATTACG453.8562575E-1045.01
AACGATC207.034504E-445.017
ACCGTAT253.8918155E-545.028
AGTCGAC207.034504E-445.041
ACGCGTA253.8918155E-545.017
TAATGCG800.042.18751
CGTTTTT56250.042.041
GTATGCG1450.041.896551
CGGTCTA1650.040.9090931
AGTACGG2700.040.8333322
TATAGCG1400.040.1785741
CGTACAC451.929584E-840.042
ATAGGGC7600.039.0789454
CGAACTA356.2505424E-638.5714345
CGAGGGT2450.038.571434
TAGTACG1600.037.968751
GCGATAT1550.037.741949
CCGTATC301.1402155E-437.49999629
GGCACCG6200.037.3790368