FastQCFastQC Report
Thu 26 May 2016
SRR1546813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546813_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2432229
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319691.3143910380149237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA253.892095E-545.018
CGTTTTT170000.043.4911771
TACTCGC451.9297659E-840.045
CGGTCTA3150.039.28571331
TCACGAC3250.038.7692325
CGTTAAC356.2511645E-638.5714338
AGTACGG3150.038.5714262
ACGGGTA2700.038.3333365
CATACGA6050.038.30578618
GTAGGGA23300.037.6609464
TACGGGT2750.037.6363644
GTTTTTT205050.037.6152152
AGGGCGA12950.037.5289576
CGATCGA301.1402965E-437.50000410
TATCGCC301.1402965E-437.50000438
TCGATAG900.037.51
CGACGGT3400.036.39705728
TAGGGAT24850.036.2173045
ACAACGA3050.036.14753713
GGGCGAT29300.036.0153587
Produced by FastQC (version 0.11.5)