FastQCFastQC Report
Thu 26 May 2016
SRR1546812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546812_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1964267
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75510.38441820791165354No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61050.3108029611045749No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22230.1131719873112973No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21930.11164470003314213No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20890.10635010413553758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA950.045.00000423
TCCGCGA207.034508E-445.00000445
TACTTCG207.034508E-445.00000429
GATCGAC207.034508E-445.0000049
TCCGATT207.034508E-445.00000428
TAGACGC207.034508E-445.00000423
ACTAGTC207.034508E-445.00000412
CTAACGA207.034508E-445.00000418
TGCGCAT207.034508E-445.0000041
CTACGTT406.8193913E-945.00000430
AATTACG207.034508E-445.0000041
ACGTAAG800.045.0000041
ATCGATC207.034508E-445.00000436
CGAATCG351.2125201E-745.035
CGTACAC253.8918202E-545.017
CCGAATA253.8918202E-545.012
ACGTAGT253.8918202E-545.044
CGACTCA351.2125201E-745.034
TATCGCG502.1827873E-1145.01
TGGTCGA351.2125201E-745.034