FastQCFastQC Report
Thu 26 May 2016
SRR1546811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546811_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2209009
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567102.5672145292300756No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30020.1358980429685891No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29020.13137112614751684No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28350.12833809187739842No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA253.8919785E-545.02
GACCGTA207.034701E-445.09
TAAGTCG253.8919785E-545.01
ATCACGT207.034701E-445.028
CTACGCG253.8919785E-545.01
CGGTCTA3350.045.031
ACTCCGT207.034701E-445.034
TGTCGAC406.8193913E-945.025
CGCTTAT207.034701E-445.022
CGTTTTT247250.044.253791
TGTTGCG1700.042.3529431
TCTAGCG603.6379788E-1241.2499961
TCGATAG1050.040.7142871
GGGCGTA501.0822987E-940.57
TCGTAAG451.9297659E-840.01
TCACGAC4000.039.937525
TGCGGGC4750.039.7894744
CGACGGT3850.039.7402628
GTTTTTT280300.039.629862
TACGGGT1250.039.64