##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546811_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2209009
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68683196854336	31.0	31.0	33.0	30.0	34.0
2	32.069412573692546	33.0	31.0	34.0	30.0	34.0
3	31.98570309129569	33.0	31.0	34.0	30.0	34.0
4	35.7717587388734	37.0	35.0	37.0	35.0	37.0
5	35.747419317893225	37.0	35.0	37.0	33.0	37.0
6	35.80857388992077	37.0	35.0	37.0	35.0	37.0
7	36.142286427986484	37.0	35.0	37.0	35.0	37.0
8	36.0408902815697	37.0	35.0	37.0	35.0	37.0
9	37.810088596289106	39.0	38.0	39.0	35.0	39.0
10	37.52040892545028	39.0	37.0	39.0	35.0	39.0
11	37.225721579223986	39.0	37.0	39.0	34.0	39.0
12	36.60044436215516	39.0	35.0	39.0	33.0	39.0
13	36.417835327968334	39.0	35.0	39.0	33.0	39.0
14	37.50461903957838	40.0	35.0	41.0	33.0	41.0
15	37.69099446856033	40.0	35.0	41.0	33.0	41.0
16	37.76530607163665	40.0	35.0	41.0	33.0	41.0
17	37.66298009650481	40.0	35.0	41.0	33.0	41.0
18	37.45822764868772	39.0	35.0	41.0	33.0	41.0
19	37.274490054137395	38.0	35.0	41.0	33.0	41.0
20	37.0522297555148	38.0	35.0	41.0	33.0	41.0
21	36.91916601516789	38.0	35.0	41.0	33.0	41.0
22	36.85318212827562	38.0	35.0	41.0	33.0	41.0
23	36.76351295988382	38.0	35.0	41.0	32.0	41.0
24	36.68973236415062	38.0	35.0	41.0	32.0	41.0
25	36.59510350568966	38.0	35.0	40.0	32.0	41.0
26	36.53211191081612	38.0	35.0	40.0	32.0	41.0
27	36.440751938991646	38.0	35.0	40.0	32.0	41.0
28	36.40249722839518	37.0	35.0	40.0	32.0	41.0
29	36.29944332503851	37.0	35.0	40.0	31.0	41.0
30	36.2094581778526	37.0	35.0	40.0	31.0	41.0
31	35.97712956352826	37.0	35.0	40.0	31.0	41.0
32	35.59132669898583	37.0	35.0	40.0	30.0	41.0
33	35.28913598812861	37.0	35.0	40.0	27.0	41.0
34	34.92349827456565	37.0	34.0	40.0	24.0	41.0
35	34.647317869687264	37.0	34.0	40.0	23.0	41.0
36	34.46561784039812	37.0	34.0	40.0	22.0	41.0
37	34.17460137102203	36.0	34.0	40.0	21.0	41.0
38	34.247369748154036	36.0	34.0	40.0	22.0	41.0
39	34.15262816946423	36.0	34.0	40.0	21.0	41.0
40	34.02534032228932	36.0	34.0	40.0	21.0	41.0
41	33.93250367019781	36.0	34.0	40.0	20.0	41.0
42	33.91652636996952	36.0	34.0	40.0	20.0	41.0
43	33.805294138683905	36.0	33.0	40.0	19.0	41.0
44	33.65286470086813	35.0	33.0	40.0	18.0	41.0
45	33.61739902372512	35.0	33.0	40.0	19.0	41.0
46	33.417957554722506	35.0	33.0	40.0	18.0	41.0
47	33.31171217500698	35.0	33.0	40.0	18.0	41.0
48	33.145081346431816	35.0	33.0	40.0	18.0	41.0
49	33.18052665244913	35.0	33.0	40.0	18.0	41.0
50	33.0491102571334	35.0	33.0	40.0	18.0	41.0
51	32.50348685768143	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	10.0
14	21.0
15	44.0
16	130.0
17	407.0
18	859.0
19	1957.0
20	3405.0
21	5690.0
22	9108.0
23	14153.0
24	22197.0
25	35604.0
26	50255.0
27	54789.0
28	51018.0
29	48742.0
30	51841.0
31	59679.0
32	73083.0
33	95999.0
34	173110.0
35	320782.0
36	158076.0
37	184061.0
38	268599.0
39	525099.0
40	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.102651007759587	23.778128563532334	28.344067407602232	20.775153021105844
2	36.52656010002676	24.846345125800756	26.705730940887975	11.921363833284518
3	26.185859813156036	23.6918455289227	37.88612902889938	12.236165629021881
4	24.734349203647426	25.12284920523185	37.09251524099721	13.050286350123516
5	21.987732960798258	29.15148829180868	35.49261229809385	13.36816644929921
6	19.83006859637059	37.015602924207194	33.11453235364817	10.039796125774046
7	79.19161940942749	4.507315271237012	14.506595491462463	1.7944698278730415
8	80.72054029657643	3.8053715489615483	13.930364249308175	1.5437239051538496
9	76.29810471573452	6.054298556502033	15.05783815276443	2.5897585749990153
10	44.716476936037836	24.026158336158883	21.322638341446325	9.93472638635696
11	38.179065816390974	21.60054576509195	27.0807407303456	13.139647688171483
12	33.92240592953673	21.31444462200018	30.770947515379067	13.99220193308402
13	20.64658858338739	33.749432437803556	31.173073536594913	14.430905442214135
14	14.866394840401284	36.80985455468946	33.458578032049665	14.865172572859594
15	14.593240679417786	25.03086225542766	46.07509521237804	14.300801852776516
16	16.189114666350385	21.79932268270523	45.33648346385189	16.675079187092493
17	17.13220724768437	22.67147847745301	33.55024809767638	26.64606617718624
18	19.52925497361034	26.458244398279952	35.934937340680825	18.077563287428887
19	24.80098541925361	26.80455353509198	30.687788053375968	17.70667299227844
20	26.515962587748625	25.539959321125444	30.615357384238816	17.328720706887115
21	21.47397317077477	27.72129946052732	32.50004866435582	18.304678704342084
22	20.345412807281456	26.13479619141434	32.1208288422546	21.3989621590496
23	19.304584091780523	29.573442208700822	30.97221423724394	20.149759462274712
24	18.545374871718494	26.73334513349651	38.07603318954337	16.645246805241626
25	17.466927477434453	27.943887960619445	36.400032774877786	18.18915178706832
26	16.495043705118448	34.06550177025082	31.401230144376957	18.03822438025377
27	17.603776172935465	32.03106008169274	33.80918773984171	16.55597600553008
28	15.231807566198235	30.154653059358296	38.616547057979396	15.996992316464079
29	15.69119908520065	27.752852070770196	38.51093408854378	18.04501475548538
30	17.612422584063715	30.25365673023514	34.99723179036391	17.13668889533723
31	24.547523346441775	28.522835352866377	29.65071667883653	17.27892462185532
32	24.482652628395808	29.380007052936406	30.329392048651684	15.807948270016103
33	22.976004172006544	30.38982638821299	29.456783562221794	17.17738587755867
34	18.463618753929932	30.970765623861197	30.79679621042739	19.768819411781482
35	19.84939853119657	28.66656496193542	31.881354942419883	19.60268156444813
36	24.45196013234894	28.946464228982315	29.49847646614387	17.103099172524875
37	19.913635480887585	32.69140143838255	30.418255425849328	16.97670765488054
38	19.581088171211615	33.42987737940407	27.30016038866297	19.688874060721346
39	20.31023866358172	31.93264491000263	28.664029888515618	19.093086537900025
40	21.415349597941884	30.131158361056926	27.97037042402272	20.483121616978472
41	17.667107739262267	28.534650605769375	29.608797429073398	24.18944422589496
42	20.2729821381443	30.521423860201562	26.69119953789233	22.514394463761807
43	20.97909062389515	30.22998095526093	27.60794546332767	21.182982957516245
44	19.278509050891145	33.52992224114976	28.062719527172593	19.128849180786496
45	18.642341430025862	36.16938636284415	25.202930363796618	19.98534184333337
46	20.513270883006815	34.23195650176165	26.977300680984094	18.277471934247437
47	21.638073905538636	30.667824350195044	28.141170995681776	19.552930748584547
48	21.54414038150139	28.119894486622734	30.252932423543772	20.08303270833211
49	19.907478874010927	28.38607719570178	30.67968487226625	21.026759058021042
50	18.169323891392022	32.95120119474389	28.919981765579045	19.959493148285045
51	18.175027806586574	33.968535211943454	27.062995216406993	20.793441765062976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2395.0
1	3338.5
2	4282.0
3	40520.5
4	76759.0
5	50116.0
6	23473.0
7	23595.5
8	23718.0
9	25651.5
10	27585.0
11	29173.0
12	30761.0
13	31029.5
14	31298.0
15	29936.5
16	28575.0
17	26705.5
18	24836.0
19	22908.0
20	20980.0
21	19754.5
22	18529.0
23	18035.0
24	17541.0
25	19424.5
26	23597.5
27	25887.0
28	30440.0
29	34993.0
30	40347.0
31	45701.0
32	47801.0
33	49901.0
34	53805.5
35	57710.0
36	60296.0
37	62882.0
38	73346.5
39	83811.0
40	106230.5
41	128650.0
42	150177.5
43	171705.0
44	181351.5
45	190998.0
46	182397.0
47	173796.0
48	166791.0
49	159786.0
50	149468.0
51	139150.0
52	126859.5
53	114569.0
54	104454.5
55	94340.0
56	87770.5
57	81201.0
58	75633.5
59	70066.0
60	63814.0
61	57562.0
62	50173.0
63	42784.0
64	34520.0
65	26256.0
66	21486.5
67	16717.0
68	13487.0
69	10257.0
70	8606.5
71	6956.0
72	6212.0
73	5468.0
74	4208.0
75	2167.5
76	1387.0
77	1150.0
78	913.0
79	596.0
80	279.0
81	201.0
82	123.0
83	97.0
84	71.0
85	66.5
86	62.0
87	37.5
88	13.0
89	12.5
90	12.0
91	10.5
92	9.0
93	5.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2209009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.163635109481284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94174733345936	19.07519577470579
2	7.2399406867862	3.498865699395781
3	2.9635461570262294	2.1483014390546202
4	1.7689055400473699	1.7097275205137832
5	1.166921470853742	1.4098532311564513
6	0.8215698054542164	1.1911267797577931
7	0.599576591910484	1.0141564990977912
8	0.4846778833636941	0.9369263615388828
9	0.37778920333614624	0.8215884411944642
>10	3.7908538044209585	21.150942379618026
>50	1.223991574330235	21.14155810520258
>100	0.6134613813542756	21.494740285490714
>500	0.004552584426212644	0.7937004247699907
>1k	0.002276292213106322	1.0139435837570434
>5k	0.0	0.0
>10k+	1.896910177588602E-4	2.599373474746256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56710	2.5672145292300756	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3002	0.1358980429685891	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2902	0.13137112614751684	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2835	0.12833809187739842	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017338091424706735	0.0
2	0.0	0.0	0.0	0.08411011453552249	0.0
3	0.0	0.0	0.0	0.14947879343180584	0.0
4	0.0	0.0	0.0	0.233317292958064	0.0
5	0.0	0.0	0.0	0.45866721231104085	0.0
6	0.0	0.0	0.0	0.8262981273503186	0.0
7	0.0	0.0	0.0	1.1108601187229206	0.0
8	0.0	0.0	0.0	1.4874090598997107	0.0
9	0.0	0.0	0.0	1.7358915242083668	0.0
10	0.0	0.0	0.0	2.02556893158878	0.0
11	0.0	0.0	0.0	2.3561243978634763	0.0
12	0.0	0.0	0.0	2.5893964216533294	0.0
13	0.0	0.0	0.0	2.7031578413668753	0.0
14	0.0	0.0	0.0	2.753949848099306	0.0
15	0.0	0.0	0.0	2.8089971566435445	0.0
16	0.0	0.0	0.0	2.9120750526593597	0.0
17	0.0	0.0	0.0	3.0329890009501996	0.0
18	0.0	0.0	0.0	3.1929249722386825	0.0
19	0.0	0.0	0.0	3.2805660818946416	0.0
20	0.0	0.0	0.0	3.3731415308855692	0.0
21	0.0	0.0	0.0	3.4867671430944824	0.0
22	0.0	0.0	0.0	3.610080357300491	0.0
23	0.0	0.0	0.0	3.7525424296596346	0.0
24	0.0	0.0	0.0	3.855439249002607	0.0
25	0.0	0.0	0.0	3.945977585424052	0.0
26	0.0	0.0	0.0	4.030676199146314	0.0
27	0.0	0.0	0.0	4.125152953202092	0.0
28	0.0	0.0	0.0	4.219358092248606	0.0
29	0.0	0.0	0.0	4.325016330852432	0.0
30	0.0	0.0	0.0	4.46394740809114	0.0
31	0.0	0.0	0.0	4.582326282962179	0.0
32	0.0	0.0	0.0	4.695318126816143	0.0
33	0.0	0.0	0.0	4.807042433960206	0.0
34	0.0	0.0	0.0	4.92071331533733	0.0
35	0.0	0.0	0.0	5.061138275126991	0.0
36	0.0	0.0	0.0	5.189114213658704	0.0
37	0.0	0.0	0.0	5.316411114667256	0.0
38	0.0	0.0	0.0	5.448416009169723	0.0
39	0.0	0.0	0.0	5.591557119052028	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTA	25	3.8919785E-5	45.0	2
GACCGTA	20	7.034701E-4	45.0	9
TAAGTCG	25	3.8919785E-5	45.0	1
ATCACGT	20	7.034701E-4	45.0	28
CTACGCG	25	3.8919785E-5	45.0	1
CGGTCTA	335	0.0	45.0	31
ACTCCGT	20	7.034701E-4	45.0	34
TGTCGAC	40	6.8193913E-9	45.0	25
CGCTTAT	20	7.034701E-4	45.0	22
CGTTTTT	24725	0.0	44.25379	1
TGTTGCG	170	0.0	42.352943	1
TCTAGCG	60	3.6379788E-12	41.249996	1
TCGATAG	105	0.0	40.714287	1
GGGCGTA	50	1.0822987E-9	40.5	7
TCGTAAG	45	1.9297659E-8	40.0	1
TCACGAC	400	0.0	39.9375	25
TGCGGGC	475	0.0	39.789474	4
CGACGGT	385	0.0	39.74026	28
GTTTTTT	28030	0.0	39.62986	2
TACGGGT	125	0.0	39.6	4
>>END_MODULE