FastQCFastQC Report
Thu 26 May 2016
SRR1546810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546810_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4687361
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212770.45392279365724125No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC115700.246833986117135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA302.1669384E-645.00000437
AATGCGC406.8230293E-945.00000420
TACGCTA302.1669384E-645.00000429
ACGATAC406.8230293E-945.00000445
TCTATCG302.1669384E-645.00000429
TACGACT253.892651E-545.021
TAATGCG3450.043.043481
CGTAAGG4150.042.2891542
CGTTTTT126000.041.3751
TCACGAC6800.041.36029425
ACTATCG603.6379788E-1241.25000436
TACGGGA7800.040.961544
AGTACGG3850.040.3246732
ATATACG950.040.2631571
AGGGCGA31800.040.2594346
GTAGGGT19300.040.1036264
AAAGTCG900.040.0000041
ATACGAC451.9304935E-840.00000410
TATCCGC451.9304935E-840.00000444
GCGTAAG3100.039.9193531
Produced by FastQC (version 0.11.5)