##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546810_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4687361
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62159005888388	31.0	31.0	33.0	30.0	34.0
2	31.989516702468617	33.0	31.0	34.0	30.0	34.0
3	32.052518890693506	33.0	31.0	34.0	30.0	34.0
4	35.753427141626176	37.0	35.0	37.0	35.0	37.0
5	35.68682762006169	37.0	35.0	37.0	33.0	37.0
6	35.737179833172654	37.0	35.0	37.0	33.0	37.0
7	36.074804991550685	37.0	35.0	37.0	35.0	37.0
8	35.96070112799078	37.0	35.0	37.0	35.0	37.0
9	37.73261628451489	39.0	38.0	39.0	35.0	39.0
10	37.41696255099618	39.0	37.0	39.0	35.0	39.0
11	37.102826302475954	39.0	37.0	39.0	33.0	39.0
12	36.19090614100343	37.0	35.0	39.0	33.0	39.0
13	35.82533178050507	37.0	35.0	39.0	32.0	39.0
14	36.71041082604903	38.0	35.0	41.0	31.0	41.0
15	36.975150196453825	38.0	35.0	41.0	32.0	41.0
16	37.115172268575	38.0	35.0	41.0	33.0	41.0
17	37.03357923573627	38.0	35.0	41.0	32.0	41.0
18	36.96252432872143	38.0	35.0	41.0	32.0	41.0
19	36.936779778643036	38.0	35.0	41.0	32.0	41.0
20	36.81034637613787	38.0	35.0	41.0	32.0	41.0
21	36.65949070276431	38.0	35.0	41.0	32.0	41.0
22	36.60152183712754	38.0	35.0	41.0	32.0	41.0
23	36.528301105888794	38.0	35.0	40.0	32.0	41.0
24	36.50779596450967	38.0	35.0	40.0	32.0	41.0
25	36.41512185641345	38.0	35.0	40.0	31.0	41.0
26	36.35686796899151	37.0	35.0	40.0	31.0	41.0
27	36.251202328986395	37.0	35.0	40.0	31.0	41.0
28	36.2584074493089	37.0	35.0	40.0	31.0	41.0
29	36.17510385907977	37.0	35.0	40.0	31.0	41.0
30	36.208435620810945	37.0	35.0	40.0	31.0	41.0
31	36.06623471074662	37.0	35.0	40.0	31.0	41.0
32	35.88524587715774	37.0	35.0	40.0	30.0	41.0
33	35.79314885284065	37.0	35.0	40.0	30.0	41.0
34	35.6219239354511	37.0	35.0	40.0	30.0	41.0
35	35.52439784347739	37.0	35.0	40.0	30.0	41.0
36	35.4145006113248	37.0	35.0	40.0	29.0	41.0
37	35.1182456397107	36.0	34.0	40.0	27.0	41.0
38	35.20734609516954	36.0	34.0	40.0	29.0	41.0
39	35.14495875184352	36.0	34.0	40.0	28.0	41.0
40	34.99798138014119	36.0	34.0	40.0	27.0	41.0
41	34.960457067420236	36.0	34.0	40.0	27.0	41.0
42	34.96705160963707	36.0	34.0	40.0	27.0	41.0
43	34.86231549052868	35.0	34.0	40.0	27.0	41.0
44	34.73895033900739	35.0	34.0	40.0	27.0	41.0
45	34.72458148625634	35.0	34.0	40.0	27.0	41.0
46	34.56120000145071	35.0	34.0	40.0	27.0	41.0
47	34.44340002828884	35.0	34.0	40.0	26.0	41.0
48	34.337775776177686	35.0	34.0	40.0	26.0	41.0
49	34.39452071218752	35.0	34.0	40.0	26.0	41.0
50	34.253193854708435	35.0	34.0	39.0	26.0	41.0
51	33.64471394458417	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	14.0
12	11.0
13	27.0
14	58.0
15	160.0
16	333.0
17	882.0
18	1840.0
19	3848.0
20	6457.0
21	10437.0
22	16284.0
23	23471.0
24	33789.0
25	47439.0
26	62995.0
27	74682.0
28	82372.0
29	92612.0
30	109804.0
31	134955.0
32	170876.0
33	231452.0
34	443569.0
35	790643.0
36	305469.0
37	392292.0
38	584541.0
39	1065783.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.90754413837552	22.84353605365578	29.87064576421573	13.378274043752977
2	31.023725290200606	24.770398524884257	30.624566787153796	13.58130939776134
3	29.62101702855829	25.27300542885432	31.094639393040136	14.011338149547262
4	26.468688884854398	26.44238410482999	31.29558401838476	15.793342991930853
5	23.544996854306717	31.11191990546493	29.654063341824966	15.689019898403386
6	22.14655538585571	39.111005105004715	27.724875468307218	11.017564040832358
7	85.52744284043837	4.822777678100748	7.226219614832312	2.4235598666285783
8	86.91609201851533	3.952330533108075	6.750045494682403	2.3815319536942003
9	83.1509457027099	4.970110046996594	8.321590762904755	3.557353487388746
10	54.2160076853479	21.072624873569584	13.467599359212997	11.24376808186952
11	46.79714235792805	20.3013380023429	19.52657369466529	13.374945945063757
12	41.32523609766775	20.912364121304076	23.117698850163237	14.644700930864937
13	23.279623651773353	36.42655216869365	24.664091372522833	15.629732807010171
14	16.5002012859688	39.259553509960085	28.507981356673827	15.732263847397288
15	15.366215659515023	23.165166924416532	46.41012288151051	15.058494534557932
16	18.325983426495206	19.09503023129646	43.774140715852695	18.80484562635564
17	19.703261600717333	18.53296556420553	28.436469902787522	33.32730293228962
18	23.129155189881896	23.41727040012493	32.58016611052573	20.873408299467442
19	29.20592205294194	24.26064474231876	25.497396082785173	21.036037121954124
20	31.17163367617728	24.291835000547216	24.93475539861342	19.601775924662086
21	24.874273605126636	25.92879020839231	28.81429443987779	20.38264174660326
22	23.863064952752733	23.840941630055802	27.303358968937957	24.992634448253508
23	21.555540526961757	27.85345528112727	26.15497291546352	24.43603127644745
24	22.115023784171946	24.35528221530196	34.470078152717484	19.059615847808605
25	19.1323646717204	24.59437197177687	34.170975950006834	22.102287406495893
26	18.342623919941307	31.837082742293582	27.75730309656116	22.062990241203952
27	19.71646732564443	31.26974858561139	29.622915751528417	19.39086833721576
28	17.026168882661267	28.039935477553364	36.18601170253369	18.747883937251686
29	17.403822748023888	25.590860187640764	35.74226521063771	21.263051853697633
30	20.111785714819064	28.137261030247085	32.161807038118035	19.589146216815816
31	28.667026072879814	26.24412755919589	24.88097247043699	20.207873897487307
32	30.516744923209455	26.281718007211307	25.669518520122516	17.532018549456723
33	27.772407544458382	26.99433647205752	25.556875179872	19.676380803612094
34	21.02903958112038	27.85345528112727	28.768639752730802	22.34886538502155
35	20.44357155337513	26.776644683437013	29.69052308964468	23.089260673543173
36	29.51517922344791	24.42666566539253	26.655937104054928	19.402218007104636
37	22.543452488511125	29.3552598146377	28.676519687730472	19.424768009120697
38	21.96562202057832	30.9595740545693	24.01592281883132	23.058881106021065
39	21.413968328874176	28.860162466684347	27.438040296021576	22.2878289084199
40	23.3771838780926	25.769148141139546	25.934870388689923	24.91879759207793
41	19.594565044168778	23.991132750389824	27.829049224072993	28.58525298136841
42	23.340852134068616	25.325508319073354	25.513609896912143	25.820029649945887
43	22.953021966944725	26.16792263279914	25.98018373238161	24.898871667874527
44	21.32148558645259	29.7382258375235	27.174715154220042	21.765573421803868
45	18.870020892352862	33.89226901875064	23.811244749444306	23.4264653394522
46	23.1210056148865	30.19400468621896	26.293857033840577	20.39113266505396
47	22.570439955446144	27.647881185170075	27.30600438071657	22.47567447866721
48	23.572026989173654	24.563651060799458	29.621038362524242	22.24328358750265
49	22.495067053721698	23.141294216511167	30.73955686365953	23.624081866107602
50	20.63425454109466	30.459633896343806	27.26133105600358	21.644780506557954
51	19.080992481697056	32.458370498879866	24.948601142519212	23.51203587690387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3527.0
1	6098.5
2	8670.0
3	22479.0
4	36288.0
5	26035.0
6	15782.0
7	16673.0
8	17564.0
9	18616.5
10	19669.0
11	19733.5
12	19798.0
13	18812.0
14	17826.0
15	17992.5
16	18159.0
17	16997.0
18	15835.0
19	16015.0
20	16195.0
21	16890.0
22	17585.0
23	19229.0
24	20873.0
25	23821.0
26	31037.5
27	35306.0
28	41126.0
29	46946.0
30	53266.5
31	59587.0
32	73500.0
33	87413.0
34	97038.5
35	106664.0
36	117877.5
37	129091.0
38	148021.5
39	166952.0
40	232162.0
41	297372.0
42	360475.5
43	423579.0
44	430288.5
45	436998.0
46	425542.5
47	414087.0
48	384524.5
49	354962.0
50	335082.5
51	315203.0
52	302748.5
53	290294.0
54	269494.0
55	248694.0
56	233035.0
57	217376.0
58	210468.0
59	203560.0
60	190535.0
61	177510.0
62	157081.0
63	136652.0
64	120042.5
65	103433.0
66	86122.0
67	68811.0
68	55228.0
69	41645.0
70	35999.0
71	30353.0
72	25149.5
73	19946.0
74	15339.5
75	8160.5
76	5588.0
77	4016.5
78	2445.0
79	1645.0
80	845.0
81	666.5
82	488.0
83	317.5
84	147.0
85	107.5
86	68.0
87	52.0
88	36.0
89	24.5
90	13.0
91	12.5
92	12.0
93	11.0
94	10.0
95	5.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4687361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.913828313408798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75059786405635	12.850511506137078
2	8.896632009846476	2.831589487445478
3	2.8433797355520913	1.3574717082420509
4	1.2830233539057914	0.8167125350460279
5	0.7422222695075493	0.5905798883665888
6	0.48154202525450907	0.45979062693548534
7	0.3461535300558444	0.38560394931723707
8	0.2440620059825975	0.31071686888265665
9	0.20807986926518834	0.298021258346648
>10	1.7696683141581349	6.176297887103137
>50	0.62807279292072	7.400043581814042
>100	1.7731871552091758	61.95871195087081
>500	0.02876570629494781	2.9084749249264727
>1k	0.004341993403010991	0.946207143622837
>5k	0.0	0.0
>10k+	2.713745876881869E-4	0.7092666829433895
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21277	0.45392279365724125	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11570	0.246833986117135	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.266793191307433E-5	0.0	0.0	0.042454592253508956	0.0
2	4.266793191307433E-5	0.0	0.0	0.1530285378062411	0.0
3	4.266793191307433E-5	0.0	0.0	0.2167530941184176	0.0
4	4.266793191307433E-5	0.0	0.0	0.3306764723263261	0.0
5	4.266793191307433E-5	0.0	0.0	0.5860013768941629	0.0
6	4.266793191307433E-5	0.0	0.0	0.8312993174624271	0.0
7	4.266793191307433E-5	0.0	0.0	1.0064298439996406	0.0
8	4.266793191307433E-5	0.0	0.0	1.3737794038052542	0.0
9	4.266793191307433E-5	0.0	0.0	1.5285359928539748	0.0
10	4.266793191307433E-5	0.0	0.0	1.8218140228584911	0.0
11	6.400189786961149E-5	0.0	0.0	2.1234976354498833	0.0
12	6.400189786961149E-5	0.0	0.0	2.3788012060517634	0.0
13	6.400189786961149E-5	0.0	0.0	2.4780681496475307	0.0
14	6.400189786961149E-5	0.0	0.0	2.518773356692604	0.0
15	6.400189786961149E-5	0.0	0.0	2.5824765790388238	0.0
16	6.400189786961149E-5	0.0	0.0	2.7171579061224427	0.0
17	6.400189786961149E-5	0.0	0.0	2.87193582913712	0.0
18	6.400189786961149E-5	0.0	0.0	3.090736983987365	0.0
19	6.400189786961149E-5	0.0	0.0	3.201737608859228	0.0
20	6.400189786961149E-5	0.0	0.0	3.3189677517904	0.0
21	6.400189786961149E-5	0.0	0.0	3.47212429339238	0.0
22	8.533586382614865E-5	0.0	0.0	3.6267955465772745	0.0
23	8.533586382614865E-5	0.0	0.0	3.793307150868047	0.0
24	8.533586382614865E-5	0.0	0.0	3.918409527237181	0.0
25	8.533586382614865E-5	0.0	0.0	4.021943264024256	0.0
26	8.533586382614865E-5	0.0	0.0	4.131002497994074	0.0
27	8.533586382614865E-5	0.0	0.0	4.247272612457201	0.0
28	8.533586382614865E-5	0.0	0.0	4.361451998256588	0.0
29	8.533586382614865E-5	0.0	0.0	4.4983947257316	0.0
30	8.533586382614865E-5	0.0	0.0	4.648329838474144	0.0
31	8.533586382614865E-5	0.0	0.0	4.7879606456596795	0.0
32	8.533586382614865E-5	0.0	0.0	4.910289606454463	0.0
33	8.533586382614865E-5	0.0	0.0	5.036437347155467	0.0
34	8.533586382614865E-5	0.0	0.0	5.178884237847266	0.0
35	8.533586382614865E-5	0.0	0.0	5.351625360197348	0.0
36	8.533586382614865E-5	0.0	0.0	5.497976366659192	0.0
37	8.533586382614865E-5	0.0	0.0	5.642663323776428	0.0
38	8.533586382614865E-5	0.0	0.0	5.816044465105206	0.0
39	8.533586382614865E-5	0.0	0.0	6.06787059925617	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACA	30	2.1669384E-6	45.000004	37
AATGCGC	40	6.8230293E-9	45.000004	20
TACGCTA	30	2.1669384E-6	45.000004	29
ACGATAC	40	6.8230293E-9	45.000004	45
TCTATCG	30	2.1669384E-6	45.000004	29
TACGACT	25	3.892651E-5	45.0	21
TAATGCG	345	0.0	43.04348	1
CGTAAGG	415	0.0	42.289154	2
CGTTTTT	12600	0.0	41.375	1
TCACGAC	680	0.0	41.360294	25
ACTATCG	60	3.6379788E-12	41.250004	36
TACGGGA	780	0.0	40.96154	4
AGTACGG	385	0.0	40.324673	2
ATATACG	95	0.0	40.263157	1
AGGGCGA	3180	0.0	40.259434	6
GTAGGGT	1930	0.0	40.103626	4
AAAGTCG	90	0.0	40.000004	1
ATACGAC	45	1.9304935E-8	40.000004	10
TATCCGC	45	1.9304935E-8	40.000004	44
GCGTAAG	310	0.0	39.919353	1
>>END_MODULE