FastQCFastQC Report
Thu 26 May 2016
SRR1546809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546809_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6726792
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429080.637867203267174No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC134130.19939668121148982No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG70210.10437367470259226No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAC253.892833E-545.035
TCGTTAG2750.043.3636361
CGTTTTT239000.042.5993731
CGTAAGG9900.040.4545442
GTATCGA451.9306754E-840.00000440
TATAGCG6200.039.9193531
CATACGA5600.039.77678718
CGTTAGG8350.039.610782
CGTCGTT403.461555E-739.37500437
CGGTCTA8400.039.37531
ACGTAAG3950.038.7341771
AGGGCGA51100.038.659496
GTCGACG356.2528106E-638.5714313
ACGCATC700.038.5714342
CAATACG700.038.571431
ACGGGAT13750.037.9636345
GGGCGAT96150.037.9563187
TATCCGT950.037.89473733
TCACGAC9050.037.5414425
CTATGCG1800.037.5000041
Produced by FastQC (version 0.11.5)