FastQCFastQC Report
Thu 26 May 2016
SRR1546808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546808_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2376793
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138350.582086870838142No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31810.13383580311789878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT302.1664855E-644.99999641
GTATGCG1150.043.043481
CGTTTTT78950.042.8910681
ATACGTT556.184564E-1140.90909245
ACGGGTC1700.039.7058835
ATTAGCG1650.039.5454521
CGGTCTA3300.039.54545231
TCGATAG800.039.3751
GACCGAT7150.039.3356639
CTCGAGG1500.039.02
CTAACGG2200.038.863642
ATTCGAC356.2511044E-638.5714318
GTACGAT356.2511044E-638.5714342
AACGGGA7050.038.2978744
CACGACG3400.037.7205926
ATAGGGA22950.037.549024
TACGGGA6600.037.54
CTATTCG301.1402889E-437.4999961
CGAGGGT3450.037.1739124
TTTAGCG1700.037.0588261
Produced by FastQC (version 0.11.5)