##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546808_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2376793
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583492967204126	31.0	31.0	33.0	30.0	34.0
2	31.958643432558073	31.0	31.0	34.0	30.0	34.0
3	32.03325405283506	33.0	31.0	34.0	30.0	34.0
4	35.761780685150114	37.0	35.0	37.0	35.0	37.0
5	35.70011776372616	37.0	35.0	37.0	33.0	37.0
6	35.755037565324365	37.0	35.0	37.0	33.0	37.0
7	36.07050508815871	37.0	35.0	37.0	35.0	37.0
8	35.94901701578556	37.0	35.0	37.0	35.0	37.0
9	37.715671915896756	39.0	38.0	39.0	35.0	39.0
10	37.357194757810205	39.0	37.0	39.0	34.0	39.0
11	37.080012436926566	39.0	37.0	39.0	33.0	39.0
12	36.403125976894074	38.0	35.0	39.0	33.0	39.0
13	36.15601821445957	38.0	35.0	39.0	32.0	39.0
14	37.12701905466736	39.0	35.0	41.0	32.0	41.0
15	37.34110332704615	39.0	35.0	41.0	33.0	41.0
16	37.438939781461826	39.0	35.0	41.0	33.0	41.0
17	37.327059192786244	39.0	35.0	41.0	33.0	41.0
18	37.23150564647405	39.0	35.0	41.0	32.0	41.0
19	37.18923061452975	39.0	35.0	41.0	32.0	41.0
20	37.09246703436101	39.0	35.0	41.0	32.0	41.0
21	36.94011047659598	38.0	35.0	41.0	32.0	41.0
22	36.89547427983842	38.0	35.0	41.0	32.0	41.0
23	36.805298568280875	38.0	35.0	41.0	32.0	41.0
24	36.78314518765412	38.0	35.0	41.0	32.0	41.0
25	36.692919829366716	38.0	35.0	41.0	32.0	41.0
26	36.64722674629217	38.0	35.0	41.0	32.0	41.0
27	36.54445843622057	38.0	35.0	41.0	31.0	41.0
28	36.53345579526698	38.0	35.0	40.0	31.0	41.0
29	36.45211257353922	38.0	35.0	40.0	31.0	41.0
30	36.45345640112538	38.0	35.0	40.0	31.0	41.0
31	36.30404709202695	38.0	35.0	40.0	31.0	41.0
32	36.1268297239179	38.0	35.0	41.0	30.0	41.0
33	36.040983375497994	38.0	35.0	41.0	30.0	41.0
34	35.837633315143556	38.0	35.0	41.0	30.0	41.0
35	35.71261948348047	38.0	35.0	40.0	29.0	41.0
36	35.61340848782372	38.0	35.0	40.0	29.0	41.0
37	35.352863290997576	38.0	34.0	40.0	27.0	41.0
38	35.45108429720216	38.0	34.0	40.0	28.0	41.0
39	35.33745260946157	37.0	34.0	40.0	27.0	41.0
40	35.21392018572926	37.0	34.0	40.0	27.0	41.0
41	35.14715585244487	37.0	34.0	40.0	27.0	41.0
42	35.15839620867278	37.0	34.0	40.0	27.0	41.0
43	35.051637227137576	37.0	34.0	40.0	27.0	41.0
44	34.91218166664072	37.0	34.0	40.0	27.0	41.0
45	34.89809041006095	36.0	34.0	40.0	27.0	41.0
46	34.71944128075099	36.0	34.0	40.0	26.0	41.0
47	34.60450236936914	36.0	34.0	40.0	25.0	41.0
48	34.47236717711639	36.0	34.0	40.0	25.0	41.0
49	34.52612448791292	36.0	34.0	40.0	26.0	41.0
50	34.391281445207895	35.0	34.0	40.0	25.0	41.0
51	33.78285445976995	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	5.0
12	10.0
13	14.0
14	43.0
15	79.0
16	239.0
17	480.0
18	1039.0
19	1982.0
20	3527.0
21	5506.0
22	8245.0
23	12278.0
24	16953.0
25	24259.0
26	32494.0
27	38578.0
28	42692.0
29	46909.0
30	54273.0
31	65541.0
32	82220.0
33	108728.0
34	200111.0
35	325054.0
36	160905.0
37	211932.0
38	328509.0
39	604053.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.202386577207186	23.849237186410427	28.70813739353827	14.24023884284412
2	31.26969828672501	25.21969729799776	30.59551252464981	12.915091890627412
3	29.46209451138572	24.566127550863705	32.42070302293889	13.55107491481168
4	26.551660157195013	27.748693302277484	30.786189626105433	14.91345691442208
5	23.542058563787425	31.563876197885133	29.952545299485482	14.94151993884196
6	22.127042615827293	39.23387522598728	27.66845913800655	10.970623020178872
7	86.46722705763607	4.520713415093363	7.0455862163848515	1.9664733108857186
8	87.84572320770046	3.3939850883101723	6.838710817475481	1.9215808865138864
9	83.31768900362799	5.363698058686642	8.460854605344261	2.8577583323411
10	48.6364609791429	25.35530018811062	15.852874019740046	10.155364813006432
11	40.98257610149474	21.91145800244279	22.31132454530117	14.794641350761298
12	36.68384247176763	21.63638146022813	25.75865883145903	15.921117236545209
13	24.39560365585055	31.557649319902907	26.452114256479213	17.594632767767322
14	17.76835424877135	34.10145519614034	30.30499500797924	17.825195547109065
15	17.917757246844804	23.93574030216346	41.79690869166983	16.34959375932191
16	21.760919019872578	21.37788187696615	38.80430479221371	18.056894310947566
17	21.63284728623822	21.659521885162064	28.546911741998564	28.160719086601148
18	24.726764173405087	24.01769947992947	31.493192718086938	19.762343628578506
19	28.389009896949375	25.820633096782093	26.19710677370726	19.59325023256127
20	30.82010086700861	24.917441274860703	25.887109226592305	18.37534863153838
21	26.110687804953987	26.0069345542502	28.622812335781873	19.25956530501394
22	24.52952360596821	23.21607308671811	28.91812623143875	23.33627707587493
23	22.322936831268013	26.96532680801399	27.913579348306733	22.79815701241126
24	22.43375842995162	24.17858854347013	33.95764797355091	19.430005053027337
25	21.006036285027765	25.054348443469838	32.6574085332631	21.2822067382393
26	20.064599651715568	31.036947685389514	27.206534182825344	21.69191848006957
27	19.548357808189436	29.512288196742414	30.765826052163565	20.173527942904578
28	18.116344166277838	27.494569363003002	35.18223926105471	19.206847209664453
29	19.27694166046433	26.009795552242032	34.420961354228155	20.29230143306548
30	21.68973065807582	27.015394272871053	31.680461866052283	19.61441320300085
31	27.90352378183544	25.19689346106287	26.71658827672414	20.182994480377552
32	29.004334832692624	26.029149362186775	26.954093183546064	18.012422621574533
33	27.505466399471896	26.02174442620792	26.504453690329782	19.9683354839904
34	21.803581548750774	27.08877045666156	29.259131947965177	21.84851604662249
35	21.642145529711673	26.772041149565823	30.353463679840857	21.232349640881644
36	27.930366674758805	26.176238317766842	26.75319222161964	19.140202785854722
37	22.59511030199096	30.39982867670849	27.417280343723665	19.587780677576884
38	22.646061310345495	30.910727185749877	24.485009843095295	21.958201660809333
39	22.564985676076965	29.560798942103915	26.49153712586666	21.382678255952452
40	24.068440120784604	26.512615949306483	26.077870475047682	23.341073454861235
41	20.29192277156656	25.594698402427134	27.351182875412373	26.76219595059393
42	23.068437175639612	26.59739405156444	25.81415377780059	24.520014994995357
43	22.720152743633964	26.818027484934532	26.166224824795425	24.295594946636076
44	21.403336344393477	28.959821069819714	27.69610142742763	21.940741158359184
45	20.675843457970466	31.87732377199024	24.742247221360884	22.70458554867841
46	23.032043598243515	29.939250073523443	26.71970171571525	20.309004612517793
47	22.417181470998948	27.862291751953155	27.413577875734234	22.30694890131366
48	23.56848072171199	25.302035137262692	29.34916923770812	21.7803149033172
49	22.070075096989935	24.175601324978658	30.76666752216116	22.987656055870243
50	20.733694520305303	29.79287636744134	27.432510950680182	22.040918161573178
51	19.994799715414846	31.27701907570411	26.20510073868444	22.523080470196604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1266.0
1	2684.5
2	4103.0
3	14455.0
4	24807.0
5	17398.5
6	9990.0
7	10007.5
8	10025.0
9	10363.0
10	10701.0
11	10802.0
12	10903.0
13	10341.5
14	9780.0
15	9652.0
16	9524.0
17	9245.5
18	8967.0
19	8625.5
20	8284.0
21	9181.5
22	10079.0
23	10492.0
24	10905.0
25	13233.5
26	19203.5
27	22845.0
28	26358.5
29	29872.0
30	34969.0
31	40066.0
32	44826.0
33	49586.0
34	57687.0
35	65788.0
36	68372.0
37	70956.0
38	81885.5
39	92815.0
40	117255.5
41	141696.0
42	163390.5
43	185085.0
44	189807.5
45	194530.0
46	186250.0
47	177970.0
48	175308.5
49	172647.0
50	169931.5
51	167216.0
52	156743.0
53	146270.0
54	136912.0
55	127554.0
56	123325.5
57	119097.0
58	113217.0
59	107337.0
60	101846.5
61	96356.0
62	86050.5
63	75745.0
64	65062.0
65	54379.0
66	44050.5
67	33722.0
68	28649.5
69	23577.0
70	19924.0
71	16271.0
72	13022.0
73	9773.0
74	7541.5
75	4213.5
76	3117.0
77	2167.5
78	1218.0
79	916.5
80	615.0
81	437.0
82	259.0
83	193.0
84	127.0
85	87.5
86	48.0
87	32.5
88	17.0
89	18.0
90	19.0
91	12.0
92	5.0
93	4.0
94	3.0
95	4.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2376793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.739338501619322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21441232687079	15.406437023049364
2	5.9892471554988385	2.244690596335068
3	1.8410365109294404	1.034994191164409
4	0.9553024359863698	0.7160694287748044
5	0.62315684836644	0.5838773560570492
6	0.4330073758645385	0.4868563074014098
7	0.3675846515133414	0.48218052488957963
8	0.3011356173661321	0.4514465814974451
9	0.23284601875250283	0.39270423277417965
>10	3.8384817055851514	18.595940511320862
>50	2.0110653299712236	27.416356908414492
>100	1.1886679054179492	30.96267508149787
>500	0.00315475834899165	0.40863264614446015
>1k	6.760196462124964E-4	0.23292525329470312
>5k	0.0	0.0
>10k+	2.2533988207083214E-4	0.584213357384376
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13835	0.582086870838142	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3181	0.13383580311789878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.207349987988016E-5	0.0	0.0	0.011191550968048122	0.0
2	4.207349987988016E-5	0.0	0.0	0.03462649040114137	0.0
3	4.207349987988016E-5	0.0	0.0	0.05326505084792828	0.0
4	4.207349987988016E-5	0.0	0.0	0.08536713125627685	0.0
5	4.207349987988016E-5	0.0	0.0	0.15979515254378485	0.0
6	4.207349987988016E-5	0.0	0.0	0.26712465073735914	0.0
7	4.207349987988016E-5	0.0	0.0	0.3460545365120143	0.0
8	4.207349987988016E-5	0.0	0.0	0.5170833135237272	0.0
9	4.207349987988016E-5	0.0	0.0	0.6039230172757998	0.0
10	4.207349987988016E-5	0.0	0.0	0.7366228358969418	0.0
11	4.207349987988016E-5	0.0	0.0	0.8607396605425882	0.0
12	4.207349987988016E-5	0.0	0.0	0.9679429382365229	0.0
13	4.207349987988016E-5	0.0	0.0	1.0119938926107575	0.0
14	4.207349987988016E-5	0.0	0.0	1.0300013505593462	0.0
15	4.207349987988016E-5	0.0	0.0	1.0553716709869139	0.0
16	4.207349987988016E-5	0.0	0.0	1.1085105013352026	0.0
17	4.207349987988016E-5	0.0	0.0	1.1726725886520197	0.0
18	4.207349987988016E-5	0.0	0.0	1.2585446019068551	0.0
19	4.207349987988016E-5	0.0	0.0	1.3109681827571857	0.0
20	4.207349987988016E-5	0.0	0.0	1.3697448620893784	0.0
21	4.207349987988016E-5	0.0	0.0	1.4421533553826522	0.0
22	4.207349987988016E-5	0.0	0.0	1.5223875196535837	0.0
23	4.207349987988016E-5	0.0	0.0	1.6060717109146652	0.0
24	4.207349987988016E-5	0.0	0.0	1.6693502547340051	0.0
25	4.207349987988016E-5	0.0	0.0	1.7257708180729243	0.0
26	4.207349987988016E-5	0.0	0.0	1.7843792034055974	0.0
27	4.207349987988016E-5	0.0	0.0	1.8425247802395917	0.0
28	4.207349987988016E-5	0.0	0.0	1.9099686005470395	0.0
29	4.207349987988016E-5	0.0	0.0	1.9875521343255387	0.0
30	4.207349987988016E-5	0.0	0.0	2.0786833350653593	0.0
31	8.414699975976032E-5	0.0	0.0	2.164723642319714	0.0
32	8.414699975976032E-5	0.0	0.0	2.241213265101336	0.0
33	8.414699975976032E-5	0.0	0.0	2.3229620753679434	0.0
34	8.414699975976032E-5	0.0	0.0	2.40832920662422	0.0
35	8.414699975976032E-5	0.0	0.0	2.5107781788317283	0.0
36	8.414699975976032E-5	0.0	0.0	2.6005630275753924	0.0
37	8.414699975976032E-5	0.0	0.0	2.6964485338016395	0.0
38	8.414699975976032E-5	0.0	0.0	2.8221220779428413	0.0
39	8.414699975976032E-5	0.0	0.0	3.02634684635978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	30	2.1664855E-6	44.999996	41
GTATGCG	115	0.0	43.04348	1
CGTTTTT	7895	0.0	42.891068	1
ATACGTT	55	6.184564E-11	40.909092	45
ACGGGTC	170	0.0	39.705883	5
ATTAGCG	165	0.0	39.545452	1
CGGTCTA	330	0.0	39.545452	31
TCGATAG	80	0.0	39.375	1
GACCGAT	715	0.0	39.335663	9
CTCGAGG	150	0.0	39.0	2
CTAACGG	220	0.0	38.86364	2
ATTCGAC	35	6.2511044E-6	38.57143	18
GTACGAT	35	6.2511044E-6	38.57143	42
AACGGGA	705	0.0	38.297874	4
CACGACG	340	0.0	37.72059	26
ATAGGGA	2295	0.0	37.54902	4
TACGGGA	660	0.0	37.5	4
CTATTCG	30	1.1402889E-4	37.499996	1
CGAGGGT	345	0.0	37.173912	4
TTTAGCG	170	0.0	37.058826	1
>>END_MODULE