FastQCFastQC Report
Thu 26 May 2016
SRR1546807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546807_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7892338
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250981.5850562913043005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT821400.044.1700171
CGGTCTA6250.039.96000331
GCGATAT8650.039.537579
ACGGGTA17500.039.4714285
TATAGCG5350.039.112151
GTTTTTT961800.038.5223052
CACGACG6500.038.42307726
GGGCGAT95650.038.295877
AGGGCGA53000.037.7405666
GGCGATA22000.037.7386368
TACGGGT22800.037.54
CGACGGT7050.037.34042728
TAGGGTA67800.037.1349565
TCACGAC6700.036.94029625
GCGGGAT31800.036.6509445
TAGGGCG21050.036.555825
GCGCGAC7900.036.4556969
CGTAAGG10050.036.2686582
TGGGCGA33400.036.2425166
ATAGGGT68850.036.241834
Produced by FastQC (version 0.11.5)