##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546807_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7892338
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609152319629494	31.0	31.0	33.0	30.0	34.0
2	32.00658423397478	33.0	31.0	34.0	30.0	34.0
3	31.976998831018133	33.0	31.0	34.0	30.0	34.0
4	35.769652668195405	37.0	35.0	37.0	35.0	37.0
5	35.73612673456205	37.0	35.0	37.0	33.0	37.0
6	35.80038665348595	37.0	35.0	37.0	35.0	37.0
7	36.1364384039305	37.0	35.0	37.0	35.0	37.0
8	36.03148420658112	37.0	35.0	37.0	35.0	37.0
9	37.841027842446685	39.0	38.0	39.0	35.0	39.0
10	37.32993556535465	39.0	37.0	39.0	34.0	39.0
11	37.18053547630626	39.0	37.0	39.0	34.0	39.0
12	36.87089985248984	39.0	35.0	39.0	33.0	39.0
13	36.75775315248789	39.0	35.0	39.0	33.0	39.0
14	37.898280332139855	40.0	36.0	41.0	33.0	41.0
15	38.02675873739822	40.0	36.0	41.0	33.0	41.0
16	38.07174375958049	40.0	36.0	41.0	33.0	41.0
17	37.96903148344635	40.0	36.0	41.0	33.0	41.0
18	37.76895211533008	39.0	36.0	41.0	33.0	41.0
19	37.604281773031005	39.0	36.0	41.0	33.0	41.0
20	37.400573949063	39.0	35.0	41.0	33.0	41.0
21	37.28754065018503	39.0	35.0	41.0	33.0	41.0
22	37.25918796179282	39.0	35.0	41.0	33.0	41.0
23	37.13483900461435	39.0	35.0	41.0	32.0	41.0
24	37.07767229431887	39.0	35.0	41.0	32.0	41.0
25	36.99733070225832	39.0	35.0	41.0	32.0	41.0
26	36.98189649252224	39.0	35.0	41.0	32.0	41.0
27	36.890343520513184	39.0	35.0	41.0	32.0	41.0
28	36.865232710509865	38.0	35.0	41.0	32.0	41.0
29	36.75605441632125	38.0	35.0	41.0	32.0	41.0
30	36.67164128044187	38.0	35.0	41.0	31.0	41.0
31	36.45645054735365	38.0	35.0	41.0	31.0	41.0
32	36.09870091726938	38.0	35.0	41.0	30.0	41.0
33	35.794347251726926	39.0	35.0	41.0	28.0	41.0
34	35.410449603146745	38.0	35.0	41.0	25.0	41.0
35	35.14950436740038	38.0	35.0	41.0	23.0	41.0
36	35.01334053356559	38.0	35.0	41.0	23.0	41.0
37	34.73830859752839	38.0	34.0	41.0	21.0	41.0
38	34.81535142058031	38.0	34.0	41.0	22.0	41.0
39	34.69830308838775	38.0	34.0	41.0	21.0	41.0
40	34.59284561811722	38.0	34.0	40.0	21.0	41.0
41	34.50218604423683	38.0	34.0	40.0	20.0	41.0
42	34.50971727262568	38.0	34.0	40.0	20.0	41.0
43	34.41585598589417	38.0	34.0	40.0	19.0	41.0
44	34.26796660254541	38.0	34.0	40.0	18.0	41.0
45	34.25520650534734	38.0	34.0	40.0	20.0	41.0
46	34.084240310032335	38.0	34.0	40.0	18.0	41.0
47	33.98698193108303	37.0	33.0	40.0	18.0	41.0
48	33.83564743425839	37.0	33.0	40.0	18.0	41.0
49	33.889818961124064	37.0	33.0	40.0	18.0	41.0
50	33.77990729743202	37.0	33.0	40.0	18.0	41.0
51	33.21499003210455	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	6.0
11	7.0
12	11.0
13	17.0
14	64.0
15	155.0
16	515.0
17	1461.0
18	3463.0
19	7125.0
20	12888.0
21	20740.0
22	31923.0
23	48112.0
24	74453.0
25	120125.0
26	171939.0
27	194335.0
28	185250.0
29	172544.0
30	175832.0
31	198154.0
32	237144.0
33	300711.0
34	492811.0
35	781333.0
36	518782.0
37	679023.0
38	1126370.0
39	2336495.0
40	545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.318688581254378	23.19575010598887	27.43584980775025	20.049711505006503
2	35.75754104803925	23.929740464739346	28.798855294844188	11.513863192377215
3	25.648470706652454	23.625686076800058	38.63406508945765	12.091778127089842
4	23.57260928257254	25.278605655257035	37.85961777105846	13.289167291111962
5	21.73652471548988	29.812838730424367	35.95427615999213	12.496360394093612
6	19.459328781914813	38.11201446263452	32.54605922858347	9.882597526867198
7	82.69586274688186	3.0729677314884385	12.984124602874331	1.2470449187553803
8	84.21301773948353	2.2941237438133038	12.421693039502363	1.071165477200799
9	80.04876375036143	3.582309830116247	14.384837547504933	1.9840888720173921
10	35.59662548664287	22.9094851234197	30.68097184889953	10.812917541037903
11	31.409678602208878	22.932393417514557	30.013552384603905	15.644375595672663
12	28.93510136033201	22.482374677820438	31.7093870029388	16.873136958908756
13	22.335954187466374	26.99269848807793	32.92550319056279	17.745844133892895
14	17.950118203249783	29.44597659147391	34.633121389377905	17.970783815898407
15	17.49707121007742	25.249108692506578	40.99026169431669	16.26355840309931
16	20.743434961857943	22.94316335666313	39.81113074478057	16.502270936698352
17	20.72881318564917	23.055183394324978	33.70830037943129	22.507703040594564
18	21.97047313483026	24.078340283956415	35.86987784861723	18.081308732596096
19	23.731180798389527	25.252795812850387	33.086228694209495	17.929794694550587
20	25.244990774596832	24.58682078745234	32.85690501344469	17.311283424506147
21	22.769222504155294	25.115738834297265	34.93502178948747	17.180016872059962
22	22.578543899158905	22.12524349565363	35.228192203628375	20.06802040155908
23	19.927517042478414	25.112026372920166	35.10414784567006	19.856308738931354
24	19.3539607654918	24.823265298571854	38.03222821931853	17.790545716617814
25	19.066593447974476	26.237687742212763	35.82696280873931	18.868756001073447
26	18.73301675625144	29.594031578475228	32.67139597923961	19.00155568603372
27	18.33366741262222	28.180863515982207	35.46167181385288	18.023797257542697
28	16.982496188075068	27.152461032459584	38.495120203924365	17.369922575540986
29	17.877630177521542	25.635584791224098	37.494326776172024	18.992458255082333
30	19.164549212159944	26.040661208377035	36.22907432499723	18.565715254465786
31	23.12162758361337	25.354806142362378	33.02068411160292	18.502882162421326
32	23.367308901367377	26.72325995161383	32.402882390490625	17.50654875652817
33	22.43833196196108	26.927077882371485	31.775539770344352	18.85905038532308
34	19.73047783812604	27.579900911491627	32.46180029289166	20.227820957490668
35	19.951008180338956	27.833602666282157	32.2090108152996	20.006378338079287
36	23.36900675059786	27.38537046943504	30.217813783444146	19.027808996522957
37	20.798627732365237	29.4878526489869	30.56152688848349	19.15199273016437
38	20.51980034306691	30.254520270165823	29.109941819521666	20.1157375672456
39	20.572674409028096	29.13766490994177	29.837191463416797	20.452469217613338
40	21.791704815480532	27.50751424989655	29.929458165628485	20.771322768994434
41	19.143009333862793	27.307662190848898	30.669986510967977	22.879341964320332
42	21.023820824703655	27.83837945105747	29.241525134883982	21.896274589354892
43	21.327773848509782	28.014271563128695	29.159230129272213	21.498724459089306
44	20.60187994989571	29.495923768090016	29.66882563823293	20.23337064378135
45	19.595055356220172	30.984912202189008	28.352181571544456	21.067850870046367
46	21.23513970131538	29.480757159665487	29.426717406172926	19.85738573284621
47	21.12235943265481	28.271964530662526	30.15636177771403	20.449314258968634
48	21.192313355053976	27.27563112476937	30.843382531260065	20.688672988916593
49	20.755953432303585	26.798079352404823	31.16949628867897	21.27647092661262
50	19.435242129771936	29.18047858568652	30.96382846249109	20.420450822050448
51	19.423445878775087	29.983535930670985	29.531363203147155	21.061654987406776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4854.0
1	8089.0
2	11324.0
3	110028.0
4	208732.0
5	146155.0
6	83578.0
7	83068.5
8	82559.0
9	84195.0
10	85831.0
11	85845.0
12	85859.0
13	82676.5
14	79494.0
15	75865.5
16	72237.0
17	67894.5
18	63552.0
19	59770.0
20	55988.0
21	54151.0
22	52314.0
23	56218.0
24	60122.0
25	65560.5
26	77790.0
27	84581.0
28	98164.5
29	111748.0
30	131966.0
31	152184.0
32	171507.0
33	190830.0
34	212264.5
35	233699.0
36	258070.0
37	282441.0
38	317011.5
39	351582.0
40	415871.0
41	480160.0
42	526688.5
43	573217.0
44	589828.5
45	606440.0
46	608839.0
47	611238.0
48	590114.0
49	568990.0
50	557984.5
51	546979.0
52	518557.5
53	490136.0
54	452161.0
55	414186.0
56	370568.5
57	326951.0
58	301281.0
59	275611.0
60	241707.5
61	207804.0
62	176124.5
63	144445.0
64	118742.5
65	93040.0
66	74025.5
67	55011.0
68	43117.5
69	31224.0
70	25175.0
71	19126.0
72	15193.0
73	11260.0
74	8904.0
75	4833.0
76	3118.0
77	2216.5
78	1315.0
79	916.0
80	517.0
81	364.0
82	211.0
83	178.5
84	146.0
85	103.0
86	60.0
87	58.5
88	57.0
89	39.5
90	22.0
91	15.0
92	8.0
93	7.0
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7892338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.74916783097823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09494264118814	16.41055968126334
2	6.963402278960336	2.7504280056360795
3	2.0671534823550926	1.2247368316626543
4	1.0346210186642235	0.8173161655622964
5	0.6552314446787083	0.6470137884547067
6	0.4824130919752	0.5716354270367616
7	0.3618227787247638	0.5001989147474382
8	0.2871068504517347	0.45360970999959005
9	0.23235736184375688	0.41299780822341225
>10	2.562697295439934	11.757161071028348
>50	0.9753210367807182	14.009759565468261
>100	1.2697427542920021	46.65732761469506
>500	0.01047279604432006	1.270108792006224
>1k	0.0025858748582094394	0.8527353336579601
>5k	6.464687145523339E-5	0.06725771043568776
>10k+	6.464687145523339E-5	1.5971535801221848
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125098	1.5850562913043005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016725081971907436	0.0
2	0.0	0.0	0.0	0.05452123312508917	0.0
3	0.0	0.0	0.0	0.0915824943128386	0.0
4	0.0	0.0	0.0	0.1282763105178719	0.0
5	0.0	0.0	0.0	0.23080613121232263	0.0
6	0.0	0.0	0.0	0.4059380122848261	0.0
7	0.0	0.0	0.0	0.5397259975434402	0.0
8	0.0	0.0	0.0	0.7742445901328605	0.0
9	0.0	0.0	0.0	0.9220841783512059	0.0
10	0.0	0.0	0.0	1.1165766088578568	0.0
11	0.0	0.0	0.0	1.2898839355334248	0.0
12	0.0	0.0	0.0	1.409658329382244	0.0
13	1.2670516645384422E-5	0.0	0.0	1.4665489491200199	0.0
14	1.2670516645384422E-5	0.0	0.0	1.496945518552297	0.0
15	2.5341033290768844E-5	0.0	0.0	1.5283937408661412	0.0
16	2.5341033290768844E-5	0.0	0.0	1.5928613295578573	0.0
17	2.5341033290768844E-5	0.0	0.0	1.665526742519137	0.0
18	2.5341033290768844E-5	0.0	0.0	1.7636092118710578	0.0
19	2.5341033290768844E-5	0.0	0.0	1.821298074157493	0.0
20	2.5341033290768844E-5	0.0	0.0	1.8817110975226858	0.0
21	2.5341033290768844E-5	0.0	0.0	1.9629037681863093	0.0
22	2.5341033290768844E-5	0.0	0.0	2.0547016612821194	0.0
23	2.5341033290768844E-5	0.0	0.0	2.152213957384998	0.0
24	3.801154993615327E-5	0.0	0.0	2.2273754621254183	0.0
25	3.801154993615327E-5	0.0	0.0	2.2994326902877193	0.0
26	3.801154993615327E-5	0.0	0.0	2.3695766704365675	0.0
27	3.801154993615327E-5	0.0	0.0	2.446942845073285	0.0
28	3.801154993615327E-5	0.0	0.0	2.5246637941760732	0.0
29	3.801154993615327E-5	0.0	0.0	2.6209597206809945	0.0
30	3.801154993615327E-5	0.0	0.0	2.7275821182519047	0.0
31	3.801154993615327E-5	0.0	0.0	2.8212172362612953	0.0
32	3.801154993615327E-5	1.2670516645384422E-5	0.0	2.912698366440971	0.0
33	3.801154993615327E-5	1.2670516645384422E-5	0.0	3.0040527914541926	0.0
34	3.801154993615327E-5	1.2670516645384422E-5	0.0	3.1008555386249297	0.0
35	3.801154993615327E-5	1.2670516645384422E-5	0.0	3.213648477802142	0.0
36	3.801154993615327E-5	1.2670516645384422E-5	0.0	3.3207270139722853	0.0
37	5.068206658153769E-5	1.2670516645384422E-5	0.0	3.42733674102655	0.0
38	5.068206658153769E-5	1.2670516645384422E-5	0.0	3.552850878915728	0.0
39	6.33525832269221E-5	1.2670516645384422E-5	0.0	3.7393862249690777	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	82140	0.0	44.170017	1
CGGTCTA	625	0.0	39.960003	31
GCGATAT	865	0.0	39.53757	9
ACGGGTA	1750	0.0	39.471428	5
TATAGCG	535	0.0	39.11215	1
GTTTTTT	96180	0.0	38.522305	2
CACGACG	650	0.0	38.423077	26
GGGCGAT	9565	0.0	38.29587	7
AGGGCGA	5300	0.0	37.740566	6
GGCGATA	2200	0.0	37.738636	8
TACGGGT	2280	0.0	37.5	4
CGACGGT	705	0.0	37.340427	28
TAGGGTA	6780	0.0	37.134956	5
TCACGAC	670	0.0	36.940296	25
GCGGGAT	3180	0.0	36.650944	5
TAGGGCG	2105	0.0	36.55582	5
GCGCGAC	790	0.0	36.455696	9
CGTAAGG	1005	0.0	36.268658	2
TGGGCGA	3340	0.0	36.242516	6
ATAGGGT	6885	0.0	36.24183	4
>>END_MODULE