FastQCFastQC Report
Thu 26 May 2016
SRR1546806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546806_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3370548
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203570.6039670700432096No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44770.13282706550982215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT207.035228E-445.00000413
CGTTTTT121100.041.9343531
TCTAGCG1250.039.6000021
CGACTAA800.039.37500428
CGGTCTA4200.038.5714331
CCCGCTA356.2518848E-638.57142629
AGGGCGA21500.038.5116236
GCGTAAG1550.037.7419361
GGGCGAT38250.037.7058837
CGTACCA301.14039E-437.50000437
TAGGGAC24700.037.348185
TCACGAC4400.037.32954825
CGACCCG5450.037.15596432
TAGACGG4800.037.0312542
TATACGG3000.036.752
CTCACGA4800.036.56250424
ATAGGGC11250.036.24
ACGGGAT7850.036.1146475
GTCGAAC250.002107685236.030
TACGGGA9750.035.5384644
Produced by FastQC (version 0.11.5)