##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546806_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3370548
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56582965143947	31.0	31.0	33.0	30.0	34.0
2	31.941427328731116	31.0	31.0	34.0	30.0	34.0
3	32.0032638609508	33.0	31.0	34.0	30.0	34.0
4	35.73239188404972	37.0	35.0	37.0	33.0	37.0
5	35.6736625616962	37.0	35.0	37.0	33.0	37.0
6	35.72824656406021	37.0	35.0	37.0	33.0	37.0
7	36.04843248041565	37.0	35.0	37.0	35.0	37.0
8	35.913684658993134	37.0	35.0	37.0	35.0	37.0
9	37.63513292200556	39.0	37.0	39.0	35.0	39.0
10	37.35357336551801	39.0	37.0	39.0	34.0	39.0
11	37.08817705607515	39.0	37.0	39.0	33.0	39.0
12	36.47031402608715	39.0	35.0	39.0	33.0	39.0
13	36.2653802883092	39.0	35.0	39.0	32.0	39.0
14	37.27531873155345	39.0	35.0	41.0	32.0	41.0
15	37.44590760908908	39.0	35.0	41.0	33.0	41.0
16	37.5218041695297	39.0	35.0	41.0	33.0	41.0
17	37.422007044551805	39.0	35.0	41.0	33.0	41.0
18	37.296849948435685	39.0	35.0	41.0	32.0	41.0
19	37.25068000811738	39.0	35.0	41.0	32.0	41.0
20	37.15782893464208	39.0	35.0	41.0	32.0	41.0
21	37.02337987769348	39.0	35.0	41.0	32.0	41.0
22	36.98168191047865	39.0	35.0	41.0	32.0	41.0
23	36.885117790934885	38.0	35.0	41.0	32.0	41.0
24	36.84890943549832	38.0	35.0	41.0	32.0	41.0
25	36.762624356632806	38.0	35.0	41.0	31.0	41.0
26	36.69657604638771	38.0	35.0	41.0	31.0	41.0
27	36.5946439570064	38.0	35.0	41.0	31.0	41.0
28	36.546796247969176	38.0	35.0	40.0	31.0	41.0
29	36.45948403642375	38.0	35.0	40.0	31.0	41.0
30	36.456540301458396	38.0	35.0	40.0	31.0	41.0
31	36.33356118945643	38.0	35.0	41.0	31.0	41.0
32	36.145717550973906	38.0	35.0	41.0	30.0	41.0
33	36.04982483560537	38.0	35.0	41.0	30.0	41.0
34	35.837375999392385	38.0	35.0	41.0	29.0	41.0
35	35.70417006374038	38.0	35.0	41.0	29.0	41.0
36	35.59848457876879	38.0	35.0	40.0	28.0	41.0
37	35.338688248913826	38.0	34.0	40.0	27.0	41.0
38	35.42830572357967	38.0	34.0	40.0	28.0	41.0
39	35.30505454899322	38.0	34.0	40.0	27.0	41.0
40	35.155545329720866	37.0	34.0	40.0	26.0	41.0
41	35.08156922850527	37.0	34.0	40.0	26.0	41.0
42	35.07639885264948	37.0	34.0	40.0	26.0	41.0
43	34.97660944155075	37.0	34.0	40.0	26.0	41.0
44	34.8400951417989	37.0	34.0	40.0	26.0	41.0
45	34.82419743021016	37.0	34.0	40.0	26.0	41.0
46	34.65669974140704	36.0	34.0	40.0	25.0	41.0
47	34.53124032056508	36.0	34.0	40.0	24.0	41.0
48	34.38074609826058	36.0	34.0	40.0	24.0	41.0
49	34.41646343561937	36.0	34.0	40.0	24.0	41.0
50	34.2811094813069	35.0	34.0	40.0	24.0	41.0
51	33.68509334387168	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	9.0
12	17.0
13	32.0
14	71.0
15	113.0
16	280.0
17	685.0
18	1538.0
19	2850.0
20	5085.0
21	8184.0
22	12530.0
23	17550.0
24	25083.0
25	35660.0
26	47625.0
27	55747.0
28	60765.0
29	66918.0
30	77755.0
31	93102.0
32	116598.0
33	154256.0
34	272134.0
35	429222.0
36	238763.0
37	316602.0
38	479908.0
39	851269.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.561501571851224	23.022606412963114	28.424279968717254	13.991612046468408
2	32.90512403324326	24.485454590766842	29.401331771569488	13.208089604420408
3	30.170168174433353	24.912655152811947	31.294584738149407	13.622591934605294
4	26.99753867917027	26.892303566067	30.643503667652855	15.466654087109871
5	23.744002458947328	31.37107081697101	29.63900825622421	15.245918467857452
6	22.39890961351092	39.53867442326885	27.255627274852635	10.806788688367588
7	85.23091793975341	4.751274866876247	7.707352038896939	2.310455154473397
8	85.75801323701666	4.0994224084629565	7.683943382500412	2.45862097201998
9	80.46724152867723	6.207744259983836	9.529132948114075	3.7958812632248526
10	48.17442148873121	25.004776671330596	15.254374066175588	11.566427773762605
11	39.566266375675404	23.04138674185919	22.455398944029277	14.936947938436123
12	34.89287201962411	22.274300796191003	26.676492961975324	16.156334222209566
13	24.217634639827114	31.017537800974797	27.30677622748586	17.458051331712234
14	18.881499388230043	33.3945103288842	30.30483470343695	17.4191555794488
15	18.262846279002705	23.235212790323708	41.522832488960255	16.979108441713336
16	21.836419478375625	21.121609898449748	38.52373560619816	18.518235016976465
17	22.518919772096407	21.127959014379858	28.606535198430638	27.746586015093094
18	24.761581796194566	23.69852024062556	31.343449195798428	20.196448767381447
19	28.27329561839796	24.71598090280868	27.177390738835346	19.833332739958013
20	29.715553672577872	24.580572654654375	26.169869113271787	19.534004559495962
21	26.29471528071993	25.16623409605797	29.004897719895993	19.534152903326106
22	24.646318640173646	23.722729953704857	28.87248601711057	22.758465389010926
23	22.978963658135115	26.27095059913106	27.962900988207256	22.787184754526564
24	22.809020966323576	24.08976225824406	33.12378283887368	19.977433936558683
25	22.04674136075202	25.246636451995343	30.78128541708945	21.925336770163188
26	20.65272472013453	29.667905634336016	27.879620761965118	21.799748883564334
27	20.93502302889619	28.46637994771177	30.337885708792754	20.260711314599288
28	19.789007603511358	26.36283476752148	34.23920976648308	19.608947862484083
29	20.57131362615219	25.946878667801197	32.849613772003835	20.63219393404277
30	22.439555822970032	26.433980468457946	30.974814777893684	20.151648930678334
31	28.0921084642616	24.482339370333843	26.75897213153469	20.666580033869863
32	28.288248676476346	26.182715688962148	26.912893689690815	18.616141944870684
33	26.83394510328884	25.72928793774781	26.792201149486672	20.644565809476678
34	22.529748871696828	27.165997932680387	29.107670325418894	21.196582870203894
35	23.005012834708182	26.600540920942233	29.173534986002274	21.220911258347307
36	27.439276936569364	26.067126176514915	26.813117629536798	19.68047925737892
37	23.338460096103066	29.545996674724705	27.09191502390709	20.023628205265137
38	24.09237310965457	29.814291325920887	24.53239057862401	21.56094498580053
39	23.235153452791653	28.2372777364393	26.555830090537206	21.971738720231844
40	24.021316415016194	25.75601949593953	27.039519983100668	23.183144105943605
41	20.62433171104521	25.49270919743614	27.638176344024767	26.24478274749388
42	23.575988236927646	26.22579473723561	26.365979656720512	23.83223736911624
43	23.185784626120146	25.69448647519632	27.185401305663053	23.934327593020484
44	21.64110999160967	28.344530325632512	27.864311678694385	22.150048004063432
45	20.954960439667378	30.784341299990388	25.87576263563076	22.38493562471147
46	23.39750094049988	28.559094841550987	27.250939609820122	20.792464608129006
47	23.307574910667345	26.806560832244493	27.963049332037404	21.922814925050762
48	23.817877686358422	25.301048968891703	28.813741860374044	22.067331484375835
49	22.647385529000033	24.79463280155037	29.795451659492763	22.762530009956837
50	21.26674950186142	29.056432366487584	27.61684450125024	22.059973630400755
51	20.94840364237507	29.952577444379962	26.292875817226157	22.806143096018808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2183.0
1	3972.5
2	5762.0
3	20634.0
4	35506.0
5	24928.0
6	14350.0
7	14679.5
8	15009.0
9	15352.5
10	15696.0
11	15455.0
12	15214.0
13	14841.5
14	14469.0
15	14149.5
16	13830.0
17	13246.0
18	12662.0
19	12378.5
20	12095.0
21	12587.5
22	13080.0
23	15714.5
24	18349.0
25	20737.0
26	26059.0
27	28993.0
28	35862.0
29	42731.0
30	47600.5
31	52470.0
32	59911.5
33	67353.0
34	76570.5
35	85788.0
36	93369.0
37	100950.0
38	115791.0
39	130632.0
40	159371.5
41	188111.0
42	217889.5
43	247668.0
44	252272.5
45	256877.0
46	252155.5
47	247434.0
48	238796.0
49	230158.0
50	234367.5
51	238577.0
52	228941.5
53	219306.0
54	201482.5
55	183659.0
56	177563.5
57	171468.0
58	164621.5
59	157775.0
60	149863.5
61	141952.0
62	130661.5
63	119371.0
64	103676.0
65	87981.0
66	74031.5
67	60082.0
68	49557.5
69	39033.0
70	32070.0
71	25107.0
72	20625.0
73	16143.0
74	12959.0
75	7435.0
76	5095.0
77	3922.5
78	2750.0
79	1902.5
80	1055.0
81	775.0
82	495.0
83	351.0
84	207.0
85	160.0
86	113.0
87	90.5
88	68.0
89	44.5
90	21.0
91	16.0
92	11.0
93	8.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3370548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.26305243280962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.49010604067104	15.065211318088007
2	6.351484610833418	2.3199499294766817
3	1.896496018435738	1.0390741866991966
4	0.9603244560307298	0.7015382357199437
5	0.6028018732540211	0.5504501108917023
6	0.44854005411526743	0.49150263159134355
7	0.34029648615757424	0.4350396798577655
8	0.2772049445029377	0.40500867488729847
9	0.22893436010916812	0.37629362001109107
>10	3.2211728592726745	14.981038584158144
>50	1.7324700368877777	23.0710755667908
>100	1.444907520194221	39.11062036289761
>500	0.003945554651592314	0.4799954963864667
>1k	0.0011507867733810918	0.3620007318403471
>5k	0.0	0.0
>10k+	1.6439811048301313E-4	0.6112008707036531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20357	0.6039670700432096	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4477	0.13282706550982215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9668766028550848E-5	0.0	0.0	0.013944320033418897	0.0
2	2.9668766028550848E-5	0.0	0.0	0.047173337985395844	0.0
3	2.9668766028550848E-5	0.0	0.0	0.07265880800392102	0.0
4	2.9668766028550848E-5	0.0	0.0	0.10787563327981088	0.0
5	2.9668766028550848E-5	0.0	0.0	0.19450843008317936	0.0
6	2.9668766028550848E-5	0.0	0.0	0.3258817260576025	0.0
7	2.9668766028550848E-5	0.0	0.0	0.4199613831341372	0.0
8	2.9668766028550848E-5	0.0	0.0	0.6442869230760102	0.0
9	2.9668766028550848E-5	0.0	0.0	0.7721889734250929	0.0
10	2.9668766028550848E-5	0.0	0.0	0.9542068530102523	0.0
11	2.9668766028550848E-5	0.0	0.0	1.1128160761988852	0.0
12	2.9668766028550848E-5	0.0	0.0	1.236030461515457	0.0
13	2.9668766028550848E-5	0.0	0.0	1.2881584834276207	0.0
14	2.9668766028550848E-5	0.0	0.0	1.311596808590176	0.0
15	2.9668766028550848E-5	0.0	0.0	1.3442324512215817	0.0
16	2.9668766028550848E-5	0.0	0.0	1.4071302352021096	0.0
17	5.9337532057101696E-5	0.0	0.0	1.4759024348562904	0.0
18	5.9337532057101696E-5	0.0	0.0	1.580633178937075	0.0
19	5.9337532057101696E-5	0.0	0.0	1.636440127836779	0.0
20	5.9337532057101696E-5	0.0	0.0	1.6971127543651656	0.0
21	5.9337532057101696E-5	0.0	0.0	1.771729700926971	0.0
22	5.9337532057101696E-5	0.0	0.0	1.8573537596853686	0.0
23	5.9337532057101696E-5	0.0	0.0	1.9491192530116765	0.0
24	5.9337532057101696E-5	0.0	0.0	2.0230834867208536	0.0
25	5.9337532057101696E-5	0.0	0.0	2.0836077694190975	0.0
26	5.9337532057101696E-5	0.0	0.0	2.149976799024966	0.0
27	5.9337532057101696E-5	0.0	0.0	2.2142690150088353	0.0
28	5.9337532057101696E-5	0.0	0.0	2.2807567196788177	0.0
29	5.9337532057101696E-5	0.0	0.0	2.356975779606165	0.0
30	5.9337532057101696E-5	0.0	0.0	2.455416745288897	0.0
31	5.9337532057101696E-5	0.0	0.0	2.5412188166434655	0.0
32	5.9337532057101696E-5	0.0	0.0	2.624113348927237	0.0
33	5.9337532057101696E-5	0.0	0.0	2.7065628497205796	0.0
34	5.9337532057101696E-5	0.0	0.0	2.795450472742118	0.0
35	5.9337532057101696E-5	0.0	0.0	2.8956715643865625	0.0
36	5.9337532057101696E-5	0.0	0.0	2.9869030199243567	0.0
37	5.9337532057101696E-5	0.0	0.0	3.0862933861200017	0.0
38	5.9337532057101696E-5	0.0	0.0	3.20016211013758	0.0
39	5.9337532057101696E-5	0.0	0.0	3.3725969782955176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	20	7.035228E-4	45.000004	13
CGTTTTT	12110	0.0	41.934353	1
TCTAGCG	125	0.0	39.600002	1
CGACTAA	80	0.0	39.375004	28
CGGTCTA	420	0.0	38.57143	31
CCCGCTA	35	6.2518848E-6	38.571426	29
AGGGCGA	2150	0.0	38.511623	6
GCGTAAG	155	0.0	37.741936	1
GGGCGAT	3825	0.0	37.705883	7
CGTACCA	30	1.14039E-4	37.500004	37
TAGGGAC	2470	0.0	37.34818	5
TCACGAC	440	0.0	37.329548	25
CGACCCG	545	0.0	37.155964	32
TAGACGG	480	0.0	37.031254	2
TATACGG	300	0.0	36.75	2
CTCACGA	480	0.0	36.562504	24
ATAGGGC	1125	0.0	36.2	4
ACGGGAT	785	0.0	36.114647	5
GTCGAAC	25	0.0021076852	36.0	30
TACGGGA	975	0.0	35.538464	4
>>END_MODULE