FastQCFastQC Report
Thu 26 May 2016
SRR1546805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546805_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3713058
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421301.134644274342065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA207.0353213E-445.00000422
CGTTTTT236650.043.583351
AGTACGG5350.040.794392
TCGATAG1450.040.3448261
TACGGGA14450.039.3944634
AGGGCGA26500.039.2264146
GGGCGAT48450.038.9164057
TAGGGAC32450.037.9969185
CGTAAGG6100.037.9918022
GACCGAT13800.037.9891329
TAACGGG12150.037.592593
TTACGGG11300.037.4336283
ATAGGGA41950.037.2765164
GCGCGAC4050.037.222229
CATACGA3700.037.09459718
TATCGCG1950.036.9230771
TTAGGGA44250.036.8644034
AGGGATT55650.036.7924546
GTTTTTT294650.036.661292
TAAGGGA38500.036.6428574
Produced by FastQC (version 0.11.5)