##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546805_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3713058
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58275739296289	31.0	31.0	33.0	30.0	34.0
2	31.964217364770494	31.0	31.0	34.0	30.0	34.0
3	31.981393773003276	33.0	31.0	34.0	30.0	34.0
4	35.73487971370229	37.0	35.0	37.0	33.0	37.0
5	35.68461602269612	37.0	35.0	37.0	33.0	37.0
6	35.74707612970226	37.0	35.0	37.0	33.0	37.0
7	36.0734865978393	37.0	35.0	37.0	35.0	37.0
8	35.95884712816228	37.0	35.0	37.0	35.0	37.0
9	37.72487340623282	39.0	38.0	39.0	35.0	39.0
10	37.40466106373776	39.0	37.0	39.0	35.0	39.0
11	37.134220095673165	39.0	37.0	39.0	33.0	39.0
12	36.620035560985045	39.0	35.0	39.0	33.0	39.0
13	36.43255559164441	39.0	35.0	39.0	32.0	39.0
14	37.4430305155481	40.0	35.0	41.0	32.0	41.0
15	37.62737479457633	40.0	35.0	41.0	33.0	41.0
16	37.70271942964532	40.0	35.0	41.0	33.0	41.0
17	37.616928687890145	40.0	35.0	41.0	33.0	41.0
18	37.46398574975128	39.0	36.0	41.0	33.0	41.0
19	37.36994951331221	39.0	35.0	41.0	33.0	41.0
20	37.2180593462316	39.0	35.0	41.0	32.0	41.0
21	37.096491624962496	39.0	35.0	41.0	32.0	41.0
22	37.07851641423323	39.0	35.0	41.0	32.0	41.0
23	36.96119559672916	38.0	35.0	41.0	32.0	41.0
24	36.91432856691169	38.0	35.0	41.0	32.0	41.0
25	36.81629131567565	38.0	35.0	41.0	32.0	41.0
26	36.7763331464254	38.0	35.0	41.0	32.0	41.0
27	36.67445431770794	38.0	35.0	41.0	31.0	41.0
28	36.6410522001003	38.0	35.0	41.0	31.0	41.0
29	36.53686395418547	38.0	35.0	40.0	31.0	41.0
30	36.50416018279273	38.0	35.0	41.0	31.0	41.0
31	36.338424823959116	38.0	35.0	41.0	31.0	41.0
32	36.08187833316905	38.0	35.0	41.0	30.0	41.0
33	35.90726269290703	38.0	35.0	41.0	29.0	41.0
34	35.627985073219975	38.0	35.0	41.0	28.0	41.0
35	35.44699571081303	38.0	35.0	41.0	27.0	41.0
36	35.32055491726766	38.0	35.0	41.0	26.0	41.0
37	35.044177063757154	38.0	34.0	40.0	24.0	41.0
38	35.12218823406475	38.0	34.0	40.0	25.0	41.0
39	35.014873454710376	38.0	34.0	40.0	24.0	41.0
40	34.891644030338334	38.0	34.0	40.0	24.0	41.0
41	34.802222319177346	38.0	34.0	40.0	23.0	41.0
42	34.819186234095994	38.0	34.0	40.0	23.0	41.0
43	34.714723012675805	37.0	34.0	40.0	23.0	41.0
44	34.56781687762486	37.0	34.0	40.0	23.0	41.0
45	34.55865758089424	37.0	34.0	40.0	23.0	41.0
46	34.3886909388434	37.0	34.0	40.0	23.0	41.0
47	34.28323177284061	37.0	34.0	40.0	23.0	41.0
48	34.14630716783848	36.0	33.0	40.0	23.0	41.0
49	34.200133151704065	36.0	33.0	40.0	23.0	41.0
50	34.07017127122711	36.0	33.0	40.0	23.0	41.0
51	33.483513319748845	35.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	10.0
12	14.0
13	20.0
14	53.0
15	106.0
16	305.0
17	790.0
18	1672.0
19	3486.0
20	5968.0
21	9419.0
22	14790.0
23	21277.0
24	30871.0
25	46081.0
26	64533.0
27	74601.0
28	76399.0
29	77640.0
30	85352.0
31	99872.0
32	123432.0
33	159337.0
34	274466.0
35	429749.0
36	255405.0
37	330311.0
38	526983.0
39	999883.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.816147229588122	23.411592277847532	28.333195980240543	16.439064512323803
2	33.92737199365052	24.42908244363541	29.17797136484267	12.465574197871405
3	28.056119780515143	23.97829497950207	34.56442102439553	13.401164215587261
4	25.515006768006316	26.80992324924631	33.456520205178585	14.218549777568787
5	22.537730355949193	30.71282484679744	32.17730506768275	14.572139729570614
6	20.70342019973833	38.971300744561496	29.806860005957354	10.518419049742826
7	84.55348664093046	3.947904934423324	9.698205629968614	1.8004027946775945
8	85.77700644589984	3.0789177007199995	9.350029005741359	1.7940468476387927
9	80.94500543756655	5.413543230404696	10.913726637181536	2.727724694847212
10	43.54634374146593	29.362832468547488	16.83149037801187	10.259333411974712
11	35.04324467864494	22.568378948026126	27.236606592194356	15.15176978113458
12	31.873970188453832	22.02876981722343	29.778123584387856	16.319136409934885
13	23.25455190842696	29.277538891124244	30.614065279885207	16.853843920563587
14	17.679443736133397	31.556873067967157	33.17152600363366	17.59215719226578
15	17.124752697103034	24.16175562030003	41.737915217052894	16.975576465544034
16	21.174675967894927	22.083172414758938	38.70774439828303	18.0344072190631
17	21.693385883010716	21.75689148944078	30.58847989985613	25.961242727692373
18	23.410514998688413	23.503268734288557	33.729879791805025	19.356336475218
19	26.16886135363358	24.788732090907278	29.21066678732193	19.831739768137208
20	27.31971867932039	25.027322492673154	29.47505802494871	18.17790080305775
21	24.340206913008092	24.624231563309813	32.26992414338801	18.765637380294088
22	23.47116581534681	22.597411621364387	32.122417694525645	21.80900486876316
23	20.87279541553081	26.363202513938646	31.211228049763832	21.55277402076671
24	20.60686905510229	25.169900389382555	35.43734032702963	18.785890228485524
25	20.56275447353637	25.977778962784853	33.206268256515244	20.25319830716353
26	19.473328991898324	29.072290279333103	29.935891117240832	21.518489611527748
27	19.477045604997283	27.90820935196811	33.017959859501254	19.596785183533356
28	17.737239763020128	26.531958294214636	36.36283085262875	19.367971090136486
29	19.01914809841376	25.555593260326127	35.29144979690595	20.133808844354167
30	21.331204629714914	25.694481475915538	33.82204102386766	19.15227287050189
31	25.757583102660934	24.885444827417185	29.936268164946522	19.42070390497536
32	26.64838523933642	25.129017645293988	29.630159291882862	18.592437823486733
33	25.100335087682446	25.844438734864898	29.056562003609965	19.99866417384269
34	20.567494501836492	27.147731061567043	31.531799395538663	20.7529750410578
35	21.11281321218252	27.07525710613731	30.877244578457976	20.934685103222193
36	25.58144796014498	26.940651075205395	28.283048635383558	19.194852329266066
37	21.992815625287836	29.05456903716559	29.3565842494246	19.596031088121972
38	22.048941869477932	29.780116550832226	26.871193501421196	21.29974807826864
39	21.21329642574934	28.34124864195496	28.35759635319459	22.08785857910111
40	23.373402731656764	25.73094737545172	28.243620218159805	22.652029674731715
41	19.77065803981516	25.67226259325871	29.033535161583796	25.523544205342336
42	22.2754398126827	26.27017945854872	28.427754158432215	23.026626570336365
43	22.593560348370534	26.55288444188052	28.332253360976317	22.52130184877263
44	21.329050071396676	28.53626310173447	28.482937783358086	21.65174904351077
45	20.39362164555469	30.29826089438948	26.817787387107877	22.49033007294796
46	22.184113471968388	28.774799639542394	28.188032613549264	20.853054274939957
47	21.512295256362815	27.663990166595838	29.286291784292086	21.537422792749265
48	21.93515425829599	26.41394236233315	30.56504907814529	21.085854301225567
49	22.03385996125027	25.36865839424	30.31708634769508	22.280395296814646
50	20.178327405604758	29.053006982384872	29.51949579026237	21.249169821748005
51	19.83594115685777	29.77192922922292	27.998054433838632	22.394075180080677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1999.0
1	3898.5
2	5798.0
3	37235.5
4	68673.0
5	47045.5
6	25418.0
7	25393.0
8	25368.0
9	25923.0
10	26478.0
11	25971.5
12	25465.0
13	24578.0
14	23691.0
15	22583.5
16	21476.0
17	20506.5
18	19537.0
19	18940.5
20	18344.0
21	18611.5
22	18879.0
23	20330.5
24	21782.0
25	26588.0
26	34982.5
27	38571.0
28	43932.5
29	49294.0
30	56561.5
31	63829.0
32	70634.0
33	77439.0
34	91089.0
35	104739.0
36	113719.5
37	122700.0
38	140549.5
39	158399.0
40	183455.0
41	208511.0
42	237708.5
43	266906.0
44	276944.5
45	286983.0
46	286564.0
47	286145.0
48	282250.0
49	278355.0
50	269757.5
51	261160.0
52	251908.5
53	242657.0
54	224589.5
55	206522.0
56	191973.5
57	177425.0
58	164228.0
59	151031.0
60	136820.0
61	122609.0
62	108335.5
63	94062.0
64	80577.0
65	67092.0
66	55879.0
67	44666.0
68	36211.5
69	27757.0
70	23033.5
71	18310.0
72	14258.5
73	10207.0
74	8369.5
75	5134.0
76	3736.0
77	2758.0
78	1780.0
79	1158.5
80	537.0
81	440.5
82	344.0
83	271.0
84	198.0
85	182.0
86	166.0
87	114.0
88	62.0
89	39.0
90	16.0
91	12.5
92	9.0
93	7.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3713058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.8531630510351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17045580408275	16.31343457056328
2	6.286840866910766	2.496273536133806
3	2.0410350883239037	1.2156300720413489
4	1.0967915602020148	0.8709912671075913
5	0.701588825615588	0.696437867486525
6	0.5172234055764737	0.6161112362832833
7	0.36642750091939785	0.5092321445495285
8	0.3236132985463841	0.5139798065219731
9	0.2578679617123711	0.4607545220562402
>10	3.4222617097253556	16.94309079998936
>50	1.6427350182433755	23.652627696937383
>100	1.1683515357902294	33.64027796646793
>500	0.003159164573794208	0.43532723977367577
>1k	0.0015109047961624471	0.4869753099397583
>5k	0.0	0.0
>10k+	1.373549814693134E-4	1.1488559641483937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42130	1.134644274342065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.693197897797449E-5	0.0	0.0	0.01475872447993002	0.0
2	2.693197897797449E-5	0.0	0.0	0.04532652061993107	0.0
3	2.693197897797449E-5	0.0	0.0	0.07982638569071639	0.0
4	2.693197897797449E-5	0.0	0.0	0.11631921720587182	0.0
5	2.693197897797449E-5	0.0	0.0	0.21217013038848304	0.0
6	2.693197897797449E-5	0.0	0.0	0.37144585406422415	0.0
7	2.693197897797449E-5	0.0	0.0	0.5052977895847574	0.0
8	2.693197897797449E-5	0.0	0.0	0.7900765352978596	0.0
9	2.693197897797449E-5	0.0	0.0	0.9731062644321742	0.0
10	2.693197897797449E-5	0.0	0.0	1.193948492051565	0.0
11	2.693197897797449E-5	0.0	0.0	1.3704068183152538	0.0
12	2.693197897797449E-5	0.0	0.0	1.5009999843794521	0.0
13	2.693197897797449E-5	0.0	0.0	1.5761940696859569	0.0
14	2.693197897797449E-5	0.0	0.0	1.6048766272974997	0.0
15	2.693197897797449E-5	0.0	0.0	1.6410462750649195	0.0
16	2.693197897797449E-5	0.0	0.0	1.6995963973630361	0.0
17	2.693197897797449E-5	0.0	0.0	1.769457950831902	0.0
18	2.693197897797449E-5	0.0	0.0	1.8703720760623723	0.0
19	2.693197897797449E-5	0.0	0.0	1.9258519527569997	0.0
20	2.693197897797449E-5	0.0	0.0	1.9865297013943763	0.0
21	2.693197897797449E-5	0.0	0.0	2.065171080010062	0.0
22	2.693197897797449E-5	0.0	0.0	2.1542351344902233	0.0
23	2.693197897797449E-5	0.0	0.0	2.2498167278830548	0.0
24	2.693197897797449E-5	0.0	0.0	2.3231525066400796	0.0
25	2.693197897797449E-5	0.0	0.0	2.386119473490584	0.0
26	2.693197897797449E-5	0.0	0.0	2.4537187407252996	0.0
27	2.693197897797449E-5	0.0	0.0	2.5323870513199633	0.0
28	2.693197897797449E-5	0.0	0.0	2.606880905173041	0.0
29	2.693197897797449E-5	0.0	0.0	2.703512845746013	0.0
30	2.693197897797449E-5	0.0	0.0	2.813664639765929	0.0
31	2.693197897797449E-5	0.0	0.0	2.9059335997444693	0.0
32	2.693197897797449E-5	0.0	0.0	3.0006803017889836	0.0
33	2.693197897797449E-5	0.0	0.0	3.091117887197022	0.0
34	2.693197897797449E-5	0.0	0.0	3.1928399717968317	0.0
35	2.693197897797449E-5	0.0	0.0	3.302264602384342	0.0
36	2.693197897797449E-5	0.0	0.0	3.3997045023266534	0.0
37	2.693197897797449E-5	0.0	0.0	3.5099640242624814	0.0
38	2.693197897797449E-5	0.0	0.0	3.6626414130886187	0.0
39	2.693197897797449E-5	0.0	0.0	3.955499752495113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAA	20	7.0353213E-4	45.000004	22
CGTTTTT	23665	0.0	43.58335	1
AGTACGG	535	0.0	40.79439	2
TCGATAG	145	0.0	40.344826	1
TACGGGA	1445	0.0	39.394463	4
AGGGCGA	2650	0.0	39.226414	6
GGGCGAT	4845	0.0	38.916405	7
TAGGGAC	3245	0.0	37.996918	5
CGTAAGG	610	0.0	37.991802	2
GACCGAT	1380	0.0	37.989132	9
TAACGGG	1215	0.0	37.59259	3
TTACGGG	1130	0.0	37.433628	3
ATAGGGA	4195	0.0	37.276516	4
GCGCGAC	405	0.0	37.22222	9
CATACGA	370	0.0	37.094597	18
TATCGCG	195	0.0	36.923077	1
TTAGGGA	4425	0.0	36.864403	4
AGGGATT	5565	0.0	36.792454	6
GTTTTTT	29465	0.0	36.66129	2
TAAGGGA	3850	0.0	36.642857	4
>>END_MODULE