FastQCFastQC Report
Thu 26 May 2016
SRR1546804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546804_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2335718
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280621.2014292821308052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT253.8920472E-545.037
CGTTTTT150550.043.3410841
ATTCGCG1400.040.178571
TACGGGC3050.037.622954
TTACGAG900.037.51
ACGTTAC301.1402829E-437.49999645
TCGATAG601.5643309E-1037.4999961
ACGAACT301.1402829E-437.49999641
GACCGAT7150.037.447559
GTTTTTT184900.037.4188732
TACGTTG1100.036.818181
GTAGGGA24450.036.7177934
CGGTCTA2150.036.62790331
TTACGGG7250.036.620693
CGTTAGG3200.036.5625042
AGGGCGA13800.036.0326086
TACGGGA6000.036.0000044
ACGGGTA1500.036.0000045
CGACGTA504.8827133E-836.030
CGGGATA2700.035.8333326
Produced by FastQC (version 0.11.5)