##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546804_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2335718
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529879035054744	31.0	31.0	33.0	30.0	34.0
2	31.912224849061403	31.0	31.0	34.0	30.0	34.0
3	31.947726994440252	33.0	31.0	34.0	30.0	34.0
4	35.71718589316005	37.0	35.0	37.0	33.0	37.0
5	35.66088371969561	37.0	35.0	37.0	33.0	37.0
6	35.712806939878874	37.0	35.0	37.0	33.0	37.0
7	36.00865686696768	37.0	35.0	37.0	35.0	37.0
8	35.87555561073726	37.0	35.0	37.0	35.0	37.0
9	37.63929763781415	39.0	37.0	39.0	35.0	39.0
10	37.35790151037069	39.0	37.0	39.0	34.0	39.0
11	37.07075169177101	39.0	37.0	39.0	33.0	39.0
12	36.52340950405828	39.0	35.0	39.0	33.0	39.0
13	36.3255726932789	39.0	35.0	39.0	32.0	39.0
14	37.36841776276074	40.0	35.0	41.0	32.0	41.0
15	37.54676977272085	40.0	35.0	41.0	33.0	41.0
16	37.633295200876134	40.0	35.0	41.0	33.0	41.0
17	37.53997229117556	40.0	35.0	41.0	33.0	41.0
18	37.38663271850454	39.0	35.0	41.0	32.0	41.0
19	37.262169919485146	39.0	35.0	41.0	32.0	41.0
20	37.09735207760526	39.0	35.0	41.0	32.0	41.0
21	36.971902858136126	38.0	35.0	41.0	32.0	41.0
22	36.92284770678652	38.0	35.0	41.0	32.0	41.0
23	36.797781667136185	38.0	35.0	41.0	32.0	41.0
24	36.75236222865945	38.0	35.0	41.0	31.0	41.0
25	36.650199210692385	38.0	35.0	41.0	31.0	41.0
26	36.58782609886981	38.0	35.0	41.0	31.0	41.0
27	36.47470114114803	38.0	35.0	40.0	31.0	41.0
28	36.418295787419545	38.0	35.0	40.0	31.0	41.0
29	36.30873975368602	38.0	35.0	40.0	31.0	41.0
30	36.27601491275916	38.0	35.0	40.0	30.0	41.0
31	36.13308413087539	38.0	35.0	40.0	30.0	41.0
32	35.870251460150584	38.0	35.0	40.0	30.0	41.0
33	35.69387571616094	38.0	35.0	40.0	29.0	41.0
34	35.41380337866129	38.0	35.0	40.0	27.0	41.0
35	35.21638185774139	38.0	34.0	40.0	26.0	41.0
36	35.078857122306715	38.0	34.0	40.0	25.0	41.0
37	34.79534729791867	37.0	34.0	40.0	24.0	41.0
38	34.848405501006546	37.0	34.0	40.0	24.0	41.0
39	34.70497508688977	37.0	34.0	40.0	24.0	41.0
40	34.55732712596298	37.0	34.0	40.0	23.0	41.0
41	34.45501854247816	37.0	34.0	40.0	23.0	41.0
42	34.429407145896896	37.0	34.0	40.0	23.0	41.0
43	34.31586861085114	36.0	33.0	40.0	23.0	41.0
44	34.16706554472757	36.0	33.0	40.0	23.0	41.0
45	34.15040343055112	36.0	33.0	40.0	23.0	41.0
46	33.96961790764125	35.0	33.0	40.0	23.0	41.0
47	33.84887259506499	35.0	33.0	40.0	23.0	41.0
48	33.689177375008455	35.0	33.0	40.0	22.0	41.0
49	33.71934796923259	35.0	33.0	40.0	23.0	41.0
50	33.581569350409595	35.0	33.0	39.0	22.0	41.0
51	32.97688162697723	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	7.0
11	4.0
12	8.0
13	15.0
14	39.0
15	91.0
16	209.0
17	572.0
18	1205.0
19	2348.0
20	4220.0
21	6604.0
22	9867.0
23	14110.0
24	20194.0
25	29860.0
26	41435.0
27	48894.0
28	50395.0
29	52077.0
30	56949.0
31	66674.0
32	81315.0
33	106741.0
34	184175.0
35	277449.0
36	176265.0
37	228887.0
38	333248.0
39	541739.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.774169655754676	21.662589405056604	28.50592408843876	18.057316850749963
2	33.26506025128033	24.068787413549067	29.217439776548364	13.44871255862223
3	27.418720924358166	25.03076141897267	33.92729773029107	13.6232199263781
4	25.44823476121689	26.25128547196194	33.15006349225377	15.150416274567391
5	23.2218529805396	30.657211187309425	31.870885098286696	14.250050733864276
6	21.68194105624052	38.36631819423407	29.062755007239744	10.88898574228567
7	82.84167866155076	4.593876486801917	10.214203940715445	2.350240910931885
8	84.07042288495444	3.5792420146610167	9.892975093739913	2.4573600066446377
9	79.4659286780339	5.206193555900156	11.573571809610577	3.7543059564553594
10	47.925177611338356	24.450340323617834	16.334163627629707	11.290318437414106
11	36.6344310400485	21.63561697088433	25.086290382657495	16.643661606409676
12	31.796817937781874	20.412995061903878	29.797860871903197	17.992326128411047
13	23.792255743201878	26.890789042170333	30.55651409973293	18.760441114894864
14	18.541921584711854	29.474833862649515	32.736914302154624	19.246330250484007
15	17.295709499177555	22.80917473770378	41.11566550414048	18.77945025897818
16	21.504907698617725	20.404903331652193	38.033486919225695	20.056702050504384
17	22.257310171861498	20.50457289792689	30.117163116437855	27.120953813773752
18	24.323998016883888	22.75972527505461	32.04650561411951	20.869771093941992
19	27.068421787219176	23.382317557170857	28.27177767179086	21.277482983819105
20	28.49042564213659	23.621430326777464	27.49073304225938	20.39741098882656
21	26.08760989126256	23.541283665236985	30.37960918227286	19.991497261227597
22	25.411757755002956	21.1658256690234	29.9784905540823	23.443926021891343
23	23.066697263967654	24.399221138853235	29.3801734627211	23.153908134458014
24	22.748037220246623	22.88422660612283	33.60859487318246	20.759141300448086
25	22.2041359444933	23.4292410299531	32.258645949553845	22.107977075999756
26	21.541727211932262	27.290066694695163	29.100302348143053	22.06790374522952
27	21.34213119905742	26.24165245975756	31.23159559501618	21.184620746168843
28	19.7571795910294	24.83835805521043	34.85994456522577	20.544517788534403
29	20.251203270257797	24.164218454453835	34.01707740403593	21.567500871252438
30	22.453052979854586	24.39545356074663	32.05712333423813	21.09437012516066
31	27.3285987435127	22.846850518769816	28.07260979279177	21.751940944925714
32	27.45310007458092	24.26744153189726	27.82883036393948	20.450628029582337
33	25.65468091610374	24.538493088634844	28.173435320530988	21.63339067473043
34	22.292502776448185	25.4530298606253	29.683934447566017	22.570532915360502
35	22.83965786965721	25.100547240719983	30.095028595061564	21.964766294561244
36	27.336519220214083	23.778255765464838	28.15382678902162	20.731398225299458
37	23.380176887792107	27.276580477609023	28.30174704309339	21.041495591505484
38	23.794524852743354	27.58261057199542	25.954717136229633	22.668147439031596
39	23.074446487118735	26.957321046461946	27.045131304378355	22.923101162040965
40	24.754443815563352	24.776920844040248	26.998293458371258	23.470341882025142
41	21.982319783467013	24.003496997497127	27.84942360336308	26.164759615672782
42	23.674561740758087	25.00002140669379	27.141033292546446	24.18438356000168
43	23.567571085208062	25.10744019611957	27.62478175875684	23.700206959915537
44	22.60568270655961	26.785510922123308	28.357019126452766	22.25178724486432
45	21.52819818145855	28.562223693099938	26.714654765686614	23.194923359754902
46	23.162171118259995	27.16034213034279	27.978463153514248	21.699023597882963
47	23.451033044228797	25.937634594587188	28.45446239657356	22.156869964610454
48	24.056885291803205	24.371777757417632	29.11190477617589	22.45943217460327
49	22.580251554340038	24.092163523165038	30.320355453869002	23.007229468625923
50	21.84677259840443	27.524812498769112	28.61330006447696	22.015114838349493
51	21.723641295738613	28.14012650499761	27.09689268995658	23.0393395093072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1901.0
1	3149.0
2	4397.0
3	24764.0
4	45131.0
5	30826.0
6	16521.0
7	16511.5
8	16502.0
9	16803.0
10	17104.0
11	16820.0
12	16536.0
13	15955.0
14	15374.0
15	14456.0
16	13538.0
17	12531.0
18	11524.0
19	11193.5
20	10863.0
21	11349.5
22	11836.0
23	11728.0
24	11620.0
25	12162.0
26	14943.0
27	17182.0
28	19686.0
29	22190.0
30	24803.0
31	27416.0
32	34000.5
33	40585.0
34	43384.5
35	46184.0
36	51668.5
37	57153.0
38	65644.5
39	74136.0
40	91796.0
41	109456.0
42	126530.5
43	143605.0
44	146063.0
45	148521.0
46	153236.0
47	157951.0
48	160057.5
49	162164.0
50	159523.0
51	156882.0
52	148488.5
53	140095.0
54	137108.0
55	134121.0
56	132596.5
57	131072.0
58	129146.5
59	127221.0
60	120303.0
61	113385.0
62	105285.5
63	97186.0
64	86155.0
65	75124.0
66	63578.5
67	52033.0
68	44698.0
69	37363.0
70	31262.0
71	25161.0
72	20235.5
73	15310.0
74	12556.0
75	7254.0
76	4706.0
77	3559.0
78	2412.0
79	1712.0
80	1012.0
81	710.5
82	409.0
83	291.0
84	173.0
85	125.0
86	77.0
87	60.5
88	44.0
89	30.0
90	16.0
91	13.5
92	11.0
93	7.5
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2335718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.964803318201305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16545476264069	17.827832500937678
2	5.556575772040815	2.440981879111181
3	1.921691960636954	1.26628757860678
4	1.1125862970395552	0.9775095675599891
5	0.736995283220476	0.809397822118991
6	0.5436495542653577	0.7164693320079831
7	0.4178806628715016	0.6425066599316482
8	0.3704899183868805	0.6510190550995426
9	0.3107616096681582	0.6143235871687865
>10	5.478208486950874	31.484663940096198
>50	2.0387656268178653	30.84734694508766
>100	0.34142057625274563	9.58135272135254
>500	0.004533866135243277	0.6710762271578196
>1k	7.884984583031785E-4	0.2542027569290637
>5k	0.0	0.0
>10k+	1.9712461457579463E-4	1.2150294268341655
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28062	1.2014292821308052	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019822598447244058	0.0
2	0.0	0.0	0.0	0.06225066553410986	0.0
3	0.0	0.0	0.0	0.10133928839012243	0.0
4	0.0	0.0	0.0	0.16761441235628616	0.0
5	0.0	0.0	0.0	0.322427621827635	0.0
6	0.0	0.0	0.0	0.5619685253099903	0.0
7	0.0	0.0	0.0	0.7591669884806299	0.0
8	0.0	0.0	0.0	1.1217535678536537	0.0
9	0.0	0.0	0.0	1.321906154766971	0.0
10	4.2813387575041164E-5	0.0	0.0	1.5730922996697376	0.0
11	4.2813387575041164E-5	0.0	0.0	1.763911568091696	0.0
12	4.2813387575041164E-5	0.0	0.0	1.931525980447982	0.0
13	4.2813387575041164E-5	0.0	0.0	2.0101313600357575	0.0
14	4.2813387575041164E-5	0.0	0.0	2.052131293246873	0.0
15	4.2813387575041164E-5	0.0	0.0	2.091134289327736	0.0
16	4.2813387575041164E-5	0.0	0.0	2.1535990217997205	0.0
17	4.2813387575041164E-5	0.0	0.0	2.224840498724589	0.0
18	4.2813387575041164E-5	0.0	0.0	2.323268476759609	0.0
19	4.2813387575041164E-5	0.0	0.0	2.382736272101341	0.0
20	4.2813387575041164E-5	0.0	0.0	2.450252984307181	0.0
21	4.2813387575041164E-5	0.0	0.0	2.530356832460083	0.0
22	4.2813387575041164E-5	0.0	0.0	2.618894917965268	0.0
23	4.2813387575041164E-5	0.0	0.0	2.7138550116067095	0.0
24	4.2813387575041164E-5	0.0	0.0	2.7903197218157327	0.0
25	4.2813387575041164E-5	0.0	0.0	2.8596345962997245	0.0
26	4.2813387575041164E-5	0.0	0.0	2.923426543786536	0.0
27	4.2813387575041164E-5	0.0	0.0	2.9893163472645243	0.0
28	4.2813387575041164E-5	0.0	0.0	3.060771891127268	0.0
29	4.2813387575041164E-5	0.0	0.0	3.1425454613955965	0.0
30	4.2813387575041164E-5	0.0	0.0	3.2450407112502453	0.0
31	4.2813387575041164E-5	0.0	0.0	3.3334931699802803	0.0
32	4.2813387575041164E-5	0.0	0.0	3.414324845721958	0.0
33	4.2813387575041164E-5	0.0	0.0	3.498495965694489	0.0
34	4.2813387575041164E-5	0.0	0.0	3.5846793148830467	0.0
35	4.2813387575041164E-5	0.0	0.0	3.6834069866310917	0.0
36	4.2813387575041164E-5	0.0	0.0	3.7714313114853764	0.0
37	4.2813387575041164E-5	0.0	0.0	3.862837893958089	0.0
38	4.2813387575041164E-5	0.0	0.0	3.974794902466822	0.0
39	4.2813387575041164E-5	0.0	0.0	4.158250268225873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAT	25	3.8920472E-5	45.0	37
CGTTTTT	15055	0.0	43.341084	1
ATTCGCG	140	0.0	40.17857	1
TACGGGC	305	0.0	37.62295	4
TTACGAG	90	0.0	37.5	1
ACGTTAC	30	1.1402829E-4	37.499996	45
TCGATAG	60	1.5643309E-10	37.499996	1
ACGAACT	30	1.1402829E-4	37.499996	41
GACCGAT	715	0.0	37.44755	9
GTTTTTT	18490	0.0	37.418873	2
TACGTTG	110	0.0	36.81818	1
GTAGGGA	2445	0.0	36.717793	4
CGGTCTA	215	0.0	36.627903	31
TTACGGG	725	0.0	36.62069	3
CGTTAGG	320	0.0	36.562504	2
AGGGCGA	1380	0.0	36.032608	6
TACGGGA	600	0.0	36.000004	4
ACGGGTA	150	0.0	36.000004	5
CGACGTA	50	4.8827133E-8	36.0	30
CGGGATA	270	0.0	35.833332	6
>>END_MODULE