FastQCFastQC Report
Thu 26 May 2016
SRR1546803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546803_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2317371
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353991.5275499693402568No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG128370.5539466921783348No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC128100.552781578780437No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC62230.2685370620414254No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40550.17498277142503293No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT37700.1626843522249998No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT24220.10451498702624656No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGA207.034771E-445.00000439
CGTTTTT205700.044.1468161
CGATGAA95700.043.8009419
CGATCGA7950.043.30188441
GATGAAT101300.043.2897320
CCGATGA91650.043.20785518
GCGATCG7450.043.1879233
TGAATGA101400.043.04733722
CCGATCG15200.042.9276340
CGCCGAT15000.042.74999638
TCGCCGA8900.042.72471637
ATGAATG102550.042.65236321
CGATCGC7400.042.56756634
CCACCGA103350.042.56168415
ATACGGT6800.042.35294328
ACCACCG105250.042.220914
ACCGATG94050.041.91387617
GCCGATC15150.041.7326739
CACCGAT98500.041.41370416
CGATCGT7200.041.2541