##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546803_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2317371
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425034662123586	31.0	31.0	33.0	30.0	34.0
2	31.767401076478475	31.0	31.0	34.0	30.0	34.0
3	31.72819285302181	31.0	31.0	34.0	30.0	34.0
4	35.56070132922178	37.0	35.0	37.0	33.0	37.0
5	35.44206042105472	37.0	35.0	37.0	33.0	37.0
6	35.54950286337406	37.0	35.0	37.0	33.0	37.0
7	35.8268904720047	37.0	35.0	37.0	35.0	37.0
8	35.65524812384378	37.0	35.0	37.0	35.0	37.0
9	37.331519208620456	39.0	37.0	39.0	34.0	39.0
10	37.10646633620598	39.0	37.0	39.0	33.0	39.0
11	36.8678890863828	39.0	37.0	39.0	33.0	39.0
12	36.2203829253063	38.0	35.0	39.0	32.0	39.0
13	35.97653634226026	38.0	35.0	39.0	31.0	39.0
14	37.05223246515124	39.0	35.0	41.0	31.0	41.0
15	37.19128702309643	39.0	35.0	41.0	32.0	41.0
16	37.32462518949275	39.0	35.0	41.0	32.0	41.0
17	37.01944531108744	39.0	35.0	41.0	32.0	41.0
18	36.91207622775982	38.0	35.0	40.0	32.0	41.0
19	36.82225720439239	38.0	35.0	40.0	32.0	41.0
20	36.21536301265529	38.0	35.0	40.0	30.0	41.0
21	36.3447298684587	37.0	35.0	40.0	31.0	41.0
22	36.1791361849268	37.0	35.0	40.0	30.0	41.0
23	35.88135736573902	37.0	35.0	40.0	30.0	41.0
24	36.09364145835949	37.0	35.0	40.0	30.0	41.0
25	36.19876834568138	37.0	35.0	40.0	31.0	41.0
26	35.955572500044234	37.0	35.0	40.0	30.0	41.0
27	35.681455407873834	37.0	35.0	40.0	30.0	41.0
28	35.65796111196697	37.0	35.0	40.0	30.0	41.0
29	35.5060881490275	36.0	35.0	40.0	29.0	41.0
30	35.4981584735461	37.0	35.0	40.0	29.0	41.0
31	35.3846574415577	37.0	34.0	40.0	29.0	41.0
32	34.77348167384506	36.0	34.0	40.0	24.0	41.0
33	34.37027088023454	36.0	34.0	40.0	22.0	41.0
34	33.76731434025886	36.0	33.0	40.0	18.0	41.0
35	33.47145191684888	36.0	33.0	40.0	15.0	41.0
36	33.374135173004234	36.0	33.0	40.0	15.0	41.0
37	33.00697946077689	36.0	33.0	40.0	12.0	41.0
38	33.15501704301987	36.0	33.0	40.0	15.0	41.0
39	32.93422589650082	36.0	33.0	40.0	12.0	41.0
40	32.808211978142474	36.0	32.0	40.0	12.0	41.0
41	32.81021769928078	35.0	32.0	40.0	13.0	41.0
42	32.77663352134812	35.0	32.0	40.0	12.0	41.0
43	32.63199634413307	35.0	32.0	40.0	11.0	41.0
44	32.62622083386734	35.0	32.0	40.0	12.0	41.0
45	32.76400628125579	35.0	32.0	40.0	15.0	41.0
46	32.503984472059074	35.0	31.0	40.0	13.0	41.0
47	32.25007303534911	35.0	31.0	40.0	11.0	41.0
48	32.33130085773922	35.0	31.0	40.0	14.0	41.0
49	32.39659165494002	35.0	31.0	39.0	15.0	41.0
50	32.15049812912995	35.0	31.0	39.0	11.0	41.0
51	31.700448913876976	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	4.0
12	8.0
13	14.0
14	27.0
15	84.0
16	258.0
17	687.0
18	1969.0
19	4069.0
20	7909.0
21	15695.0
22	26206.0
23	35791.0
24	42811.0
25	51669.0
26	61969.0
27	62776.0
28	57742.0
29	54550.0
30	57815.0
31	67119.0
32	81271.0
33	105397.0
34	181745.0
35	300207.0
36	153761.0
37	185715.0
38	273588.0
39	486388.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.76052345524303	20.382580087521593	25.47071660083776	18.386179856397618
2	33.812799072742344	29.004074013181313	26.13327775310902	11.049849160967321
3	25.561768055266075	28.80371766109095	33.78945365243632	11.845060631206657
4	22.649416084002088	24.190300128896062	40.28539236919768	12.87489141790417
5	26.4989075983086	27.820966086138128	32.5825687816064	13.097557533946874
6	19.239819605924126	40.42278944545349	30.89967035921309	9.437720589409292
7	75.50603679773329	4.029307348715419	18.624251360701415	1.8404044928498717
8	75.82303394665766	9.170607554854186	12.780172013889878	2.2261864845982795
9	71.99952014588946	5.022372334857042	13.714808720744326	9.263298798509172
10	48.364590736658045	20.57150106737333	20.431212783796813	10.63269541217181
11	42.00652377198127	19.89664149590204	24.758659705329876	13.33817502678682
12	32.00005523500553	19.42675557776463	29.283528619284528	19.28966056794531
13	25.36861814530345	30.467887964421752	29.872299256355582	14.291194633919213
14	15.975085560318137	37.37010603826491	31.383019809948426	15.271788591468521
15	14.48145333656113	22.08778827386724	44.84150358315522	18.58925480641641
16	16.823676485120423	19.828503938299047	41.17933641182184	22.168483164758683
17	17.610947923314825	24.30495591771883	31.70299447088964	26.381101688076704
18	20.21514897700886	22.878468747559193	34.08133613478377	22.825046140648176
19	24.28752236909843	24.675203064161934	28.423718083983964	22.613556482755676
20	30.917190212529626	23.091209823545732	29.16952011568281	16.82207984824182
21	21.31846821247008	29.891243137158447	30.87843940396251	17.91184924640897
22	21.408311401152428	22.2503863213961	34.390997384536185	21.95030489291529
23	23.612058664754155	26.675832225396796	30.312884730153268	19.399224379695784
24	19.79078878608561	27.320614610263092	35.094769029214575	17.793827574436722
25	17.336671598980054	30.13993011908753	33.77896763185523	18.744430650077177
26	17.057432754617192	29.00019030185499	34.900324548809834	19.042052394717977
27	22.83056964120117	27.744456973009502	31.75879045694453	17.666182928844798
28	15.616705309594364	30.23818801564359	37.14985645371414	16.995250221047904
29	17.896012334667173	23.69094115702665	40.21863568673294	18.19441082157324
30	18.251587682766377	29.809814656349804	34.228658251095744	17.709939409788074
31	24.994271525793668	23.355906326608903	28.30138117720469	23.34844097039274
32	30.537449549511063	25.353169604694287	28.223145970153247	15.886234875641406
33	27.833264505338164	25.114839186302063	28.39761091340144	18.65428539495834
34	20.658237286994616	25.82176095238958	30.90260471888187	22.61739704173393
35	23.47047581073553	25.635299656377853	30.82393798834973	20.07028654453689
36	25.41336713025234	28.278122061594797	27.565763099650425	18.742747708502435
37	21.25102109243621	29.243742154363716	30.74130124179512	18.76393551140495
38	20.709976952330898	29.996189647665396	25.798286075039346	23.495547324964367
39	21.84117260464552	29.580201012267782	28.17770654763523	20.400919835451468
40	22.174179274703963	25.993981973538116	27.573487369955007	24.258351381802914
41	18.004108966583253	26.290050233648387	27.968806030626947	27.73703476914141
42	22.677206196159354	27.011945864516303	26.75747646794579	23.553371471378558
43	22.349162046129	28.05183114831419	25.96601925198857	23.632987553568245
44	20.628893690306818	29.076138434458702	28.85688135391355	21.43808652132093
45	19.48738462680339	31.29718978963662	25.459626447383695	23.755799136176297
46	23.79118406159394	31.078579994312523	26.228212918863658	18.902023025229884
47	20.70311572898772	28.437009007189612	29.83549030345163	21.02438496037104
48	22.548741655954096	25.543600916728483	29.603158061441174	22.304499365876246
49	21.46812055557785	24.34340465984946	31.63162048718138	22.55685429739131
50	19.639108282618537	29.86189954047064	28.576520548500866	21.922471628409955
51	19.211036989761244	30.130393450164	27.59976714992981	23.058802410144942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1589.0
1	2916.5
2	4244.0
3	29812.5
4	55381.0
5	37997.5
6	20614.0
7	20792.0
8	20970.0
9	22428.0
10	23886.0
11	24258.5
12	24631.0
13	24244.0
14	23857.0
15	22625.0
16	21393.0
17	19963.5
18	18534.0
19	17571.5
20	16609.0
21	16255.0
22	15901.0
23	15078.0
24	14255.0
25	15208.0
26	18660.5
27	21160.0
28	23507.0
29	25854.0
30	27370.5
31	28887.0
32	34962.5
33	41038.0
34	46630.0
35	52222.0
36	53318.0
37	54414.0
38	66679.5
39	78945.0
40	102725.0
41	126505.0
42	145744.0
43	164983.0
44	171633.0
45	178283.0
46	198432.5
47	218582.0
48	190195.5
49	161809.0
50	159535.5
51	157262.0
52	149226.5
53	141191.0
54	135994.5
55	130798.0
56	126066.0
57	121334.0
58	108599.0
59	95864.0
60	85714.5
61	75565.0
62	66106.0
63	56647.0
64	48508.0
65	40369.0
66	32513.5
67	24658.0
68	20403.5
69	16149.0
70	13480.0
71	10811.0
72	8988.0
73	7165.0
74	5675.5
75	3312.5
76	2439.0
77	1746.0
78	1053.0
79	829.0
80	605.0
81	483.0
82	361.0
83	237.0
84	113.0
85	74.0
86	35.0
87	29.5
88	24.0
89	22.5
90	21.0
91	15.0
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2317371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.257554966063637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18426894880567	19.935894213714104
2	6.299315065825173	3.0561196291561377
3	2.33716446899056	1.7008168671380834
4	1.300326391675205	1.2617095567953789
5	0.8281559462741083	1.0044519193608312
6	0.6130007370733339	0.8921939444276352
7	0.43603270785976384	0.7403961164536853
8	0.35621142558099744	0.6912654588456745
9	0.2899589873373092	0.6330326465915034
>10	3.394510236102514	19.367822372262044
>50	1.2442413352410888	21.61321740310294
>100	0.7088350559189814	24.409647774565965
>500	0.005077350291548795	0.7487937637976398
>1k	0.0021760072678066265	0.9856647276730021
>5k	1.8133393898388554E-4	0.27373222064928115
>10k+	5.440018169516566E-4	2.6852413854661763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35399	1.5275499693402568	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	12837	0.5539466921783348	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	12810	0.552781578780437	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	6223	0.2685370620414254	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4055	0.17498277142503293	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	3770	0.1626843522249998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	2422	0.10451498702624656	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.315234807029172E-5	0.0	0.0	0.026366084670948243	0.0
2	4.315234807029172E-5	0.0	0.0	0.1346353259793102	0.0
3	4.315234807029172E-5	0.0	0.0	0.19880286755983398	0.0
4	4.315234807029172E-5	0.0	0.0	0.4302720626088788	0.0
5	4.315234807029172E-5	0.0	0.0	1.1741753909926378	0.0
6	4.315234807029172E-5	0.0	0.0	1.434686116292989	0.0
7	4.315234807029172E-5	0.0	0.0	1.6926508530571929	0.0
8	4.315234807029172E-5	0.0	0.0	2.037955942315667	0.0
9	4.315234807029172E-5	0.0	0.0	2.191448844401695	0.0
10	4.315234807029172E-5	0.0	0.0	2.9710823169876552	0.0
11	4.315234807029172E-5	0.0	0.0	3.169842032199419	0.0
12	4.315234807029172E-5	0.0	0.0	3.636620981275765	0.0
13	4.315234807029172E-5	0.0	0.0	3.739798245511832	0.0
14	4.315234807029172E-5	0.0	0.0	3.79123584441162	0.0
15	4.315234807029172E-5	4.315234807029172E-5	0.0	3.890529397321361	0.0
16	4.315234807029172E-5	4.315234807029172E-5	0.0	3.984860430203019	0.0
17	4.315234807029172E-5	4.315234807029172E-5	0.0	4.07677493159274	0.0
18	4.315234807029172E-5	4.315234807029172E-5	0.0	4.198248791410611	0.0
19	4.315234807029172E-5	4.315234807029172E-5	0.0	4.331373785207461	0.0
20	4.315234807029172E-5	4.315234807029172E-5	0.0	4.413233789496805	0.0
21	4.315234807029172E-5	4.315234807029172E-5	0.0	4.505450357323018	0.0
22	4.315234807029172E-5	4.315234807029172E-5	0.0	4.631670975428621	0.0
23	4.315234807029172E-5	4.315234807029172E-5	0.0	4.747535029997355	0.0
24	4.315234807029172E-5	4.315234807029172E-5	0.0	4.83996735956392	0.0
25	4.315234807029172E-5	4.315234807029172E-5	0.0	4.917727890786585	0.0
26	4.315234807029172E-5	4.315234807029172E-5	0.0	4.998983762202944	0.0
27	4.315234807029172E-5	4.315234807029172E-5	0.0	5.114157379202553	0.0
28	4.315234807029172E-5	4.315234807029172E-5	0.0	5.195413250618913	0.0
29	4.315234807029172E-5	4.315234807029172E-5	0.0	5.286464705047228	0.0
30	4.315234807029172E-5	4.315234807029172E-5	0.0	5.409448897047559	0.0
31	4.315234807029172E-5	4.315234807029172E-5	0.0	5.503693625233077	0.0
32	4.315234807029172E-5	4.315234807029172E-5	0.0	5.605748928419317	0.0
33	4.315234807029172E-5	4.315234807029172E-5	0.0	5.700036808952904	0.0
34	4.315234807029172E-5	4.315234807029172E-5	0.0	5.802782549708269	0.0
35	4.315234807029172E-5	4.315234807029172E-5	0.0	5.913166256072075	0.0
36	4.315234807029172E-5	4.315234807029172E-5	0.0	6.016818196136915	0.0
37	4.315234807029172E-5	4.315234807029172E-5	0.0	6.123577105262817	0.0
38	4.315234807029172E-5	4.315234807029172E-5	0.0	6.242073453063838	0.0
39	4.315234807029172E-5	4.315234807029172E-5	0.0	6.3859865338782615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTCGA	20	7.034771E-4	45.000004	39
CGTTTTT	20570	0.0	44.146816	1
CGATGAA	9570	0.0	43.80094	19
CGATCGA	795	0.0	43.301884	41
GATGAAT	10130	0.0	43.28973	20
CCGATGA	9165	0.0	43.207855	18
GCGATCG	745	0.0	43.18792	33
TGAATGA	10140	0.0	43.047337	22
CCGATCG	1520	0.0	42.92763	40
CGCCGAT	1500	0.0	42.749996	38
TCGCCGA	890	0.0	42.724716	37
ATGAATG	10255	0.0	42.652363	21
CGATCGC	740	0.0	42.567566	34
CCACCGA	10335	0.0	42.561684	15
ATACGGT	680	0.0	42.352943	28
ACCACCG	10525	0.0	42.2209	14
ACCGATG	9405	0.0	41.913876	17
GCCGATC	1515	0.0	41.73267	39
CACCGAT	9850	0.0	41.413704	16
CGATCGT	720	0.0	41.25	41
>>END_MODULE