FastQCFastQC Report
Thu 26 May 2016
SRR1546802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546802_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5653096
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445440.7879576076542836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56640.10019288545604038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT321700.043.5662081
TAGTACG2600.038.942311
CGAGGGT22000.038.6590924
GGGCGAT79650.038.6158187
TAGGGCG20400.038.3823555
ATAGGGC30150.038.358214
CGGGTCA13650.038.241766
GCGCGAC5100.037.9411779
AAGGGTC46150.037.5893825
TACGGGT12700.037.5590554
ACGGGAT17950.037.353765
TAGGGTC47650.037.350475
TCGATAG2800.036.9642831
GCGGGTC18250.036.8630145
GTTTTTT395950.036.856932
GGCGATA20400.036.8382348
ACCGCTC2450.036.73469518
AGGGCGA46300.036.6900636
GCGATAT5950.036.680679
GTTACGG8250.036.5454522
Produced by FastQC (version 0.11.5)