##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546802_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5653096
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5565960670047	31.0	31.0	33.0	30.0	34.0
2	31.94391144250867	31.0	31.0	34.0	30.0	34.0
3	31.99807079165116	33.0	31.0	34.0	30.0	34.0
4	35.75686809493418	37.0	35.0	37.0	35.0	37.0
5	35.70696941994263	37.0	35.0	37.0	33.0	37.0
6	35.76649573967964	37.0	35.0	37.0	33.0	37.0
7	36.098020093768085	37.0	35.0	37.0	35.0	37.0
8	35.99272699419929	37.0	35.0	37.0	35.0	37.0
9	37.8019028864891	39.0	38.0	39.0	35.0	39.0
10	37.28970160775617	39.0	37.0	39.0	34.0	39.0
11	37.134584659450326	39.0	37.0	39.0	34.0	39.0
12	36.72139921204239	39.0	35.0	39.0	33.0	39.0
13	36.5739062276671	39.0	35.0	39.0	33.0	39.0
14	37.65566036734561	40.0	36.0	41.0	33.0	41.0
15	37.79914209841828	40.0	36.0	41.0	33.0	41.0
16	37.84258077343813	40.0	35.0	41.0	33.0	41.0
17	37.73392562234924	40.0	35.0	41.0	33.0	41.0
18	37.62581813576136	39.0	36.0	41.0	33.0	41.0
19	37.545204256216415	39.0	36.0	41.0	33.0	41.0
20	37.43135973632855	39.0	35.0	41.0	33.0	41.0
21	37.32195667648312	39.0	35.0	41.0	33.0	41.0
22	37.279403357027725	39.0	35.0	41.0	33.0	41.0
23	37.16430288818729	39.0	35.0	41.0	32.0	41.0
24	37.14688376068618	39.0	35.0	41.0	32.0	41.0
25	37.05064375344059	39.0	35.0	41.0	32.0	41.0
26	37.023476693125325	39.0	35.0	41.0	32.0	41.0
27	36.94107865849085	39.0	35.0	41.0	32.0	41.0
28	36.92394503825868	39.0	35.0	41.0	32.0	41.0
29	36.846559124416075	38.0	35.0	41.0	32.0	41.0
30	36.82088965055608	39.0	35.0	41.0	32.0	41.0
31	36.68304429997297	39.0	35.0	41.0	31.0	41.0
32	36.441780751644764	39.0	35.0	41.0	30.0	41.0
33	36.29491503416889	39.0	35.0	41.0	30.0	41.0
34	36.037258167913656	39.0	35.0	41.0	30.0	41.0
35	35.87447745447804	38.0	35.0	41.0	29.0	41.0
36	35.75833826278556	38.0	35.0	41.0	28.0	41.0
37	35.49869628960838	38.0	35.0	41.0	27.0	41.0
38	35.60214845104346	38.0	35.0	41.0	27.0	41.0
39	35.508774307034585	38.0	35.0	40.0	27.0	41.0
40	35.41581869474709	38.0	35.0	40.0	26.0	41.0
41	35.3528659339944	38.0	35.0	40.0	26.0	41.0
42	35.366812627982966	38.0	35.0	40.0	26.0	41.0
43	35.27209797958499	38.0	35.0	40.0	26.0	41.0
44	35.141760019642334	38.0	34.0	40.0	26.0	41.0
45	35.12100360581175	38.0	34.0	40.0	26.0	41.0
46	34.955622016679	38.0	34.0	40.0	24.0	41.0
47	34.85125867312354	38.0	34.0	40.0	24.0	41.0
48	34.732825694097535	37.0	34.0	40.0	24.0	41.0
49	34.785401486194466	37.0	34.0	40.0	24.0	41.0
50	34.652353683715965	37.0	34.0	40.0	24.0	41.0
51	34.065030914033656	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	8.0
12	6.0
13	15.0
14	25.0
15	97.0
16	254.0
17	691.0
18	1704.0
19	3540.0
20	6586.0
21	10837.0
22	17243.0
23	26303.0
24	39726.0
25	61084.0
26	86913.0
27	101108.0
28	103964.0
29	106926.0
30	118879.0
31	142344.0
32	178269.0
33	233813.0
34	395570.0
35	630624.0
36	385011.0
37	510110.0
38	838914.0
39	1652261.0
40	267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.585134234408894	23.30404436790035	30.451101484920827	15.659719912769923
2	31.77736588941705	24.978631178384376	30.552267288579564	12.691735643619001
3	27.484337785878747	24.454741260364234	34.9398276625764	13.121093291180621
4	25.27268243808349	26.528366049329428	33.93039849314429	14.268553019442798
5	22.654276523872934	31.563536157885874	32.532350414710805	13.249836903530385
6	20.508797303283018	40.0178415508953	29.000675028338453	10.472686117483233
7	87.7009164535681	3.051336824989351	7.983253778106722	1.2644929433358287
8	89.14895483819839	2.0970632729392884	7.592494449059418	1.1614874398028974
9	84.5922128334633	3.458122770248374	9.616217378937135	2.3334470173512
10	38.17159659061159	22.566324718349026	25.07174829509352	14.19033039594587
11	34.10145520260049	21.169992513836668	26.16716574422228	18.561386539340567
12	31.795709819893382	23.228422089417904	27.41879847786063	17.557069612828087
13	23.155771633809156	30.169627404169326	28.85247305193473	17.82212791008679
14	18.541857417599136	32.37919540018425	31.25137093019471	17.827576252021903
15	18.386791945510918	24.991526766925592	40.33800239727045	16.283678890293036
16	20.94747727616867	22.72991649177725	38.69426593852289	17.628340293531192
17	21.214888266535716	22.151967700530825	31.45016465313874	25.182979379794716
18	22.651233943311773	24.174753091049578	33.5402229150186	19.63379005062005
19	25.342060350646793	24.69844842542918	30.338437557048387	19.62105366687564
20	26.681432616746648	25.342431828506008	29.614497967131637	18.361637587615707
21	23.70578882792721	25.857335520217596	32.02289506493433	18.413980586920868
22	23.297145493372128	22.61932222626327	32.05183495910913	22.031697321255468
23	20.33059406739245	26.528100708001425	31.98042276303109	21.160882461575035
24	20.973445347469777	25.09688496356687	35.64568866334483	18.28398102561853
25	19.23517661826369	26.77462049114326	34.36304637317321	19.627156517419834
26	18.947511239858656	30.964130097914488	29.957690440777938	20.130668221448918
27	19.15042659809775	29.361114688305314	32.404968887844824	19.08348982575212
28	17.433367485710484	27.526703951250788	36.84303256127262	18.19689600176611
29	18.627120430999224	25.452283138301564	36.2736100713662	19.64698635933301
30	19.84944179260356	27.212982054435304	33.780569089928775	19.157007063032363
31	24.837133492868332	25.893068152389414	29.69737290857965	19.572425446162597
32	24.841166681054062	26.554334120630536	30.274737948904455	18.329761249410943
33	24.051139411041312	26.971521445947495	29.911644875657515	19.065694267353678
34	20.432626652722686	27.58297400221047	31.26189613620572	20.722503208861127
35	20.51440485001493	27.470946893525245	31.58460425932976	20.430043997130067
36	24.968565897341914	26.471795278198	29.648532414804208	18.91110640965588
37	20.80256553223225	29.780424744246336	30.041998225397197	19.375011498124213
38	20.833840430093527	30.32322465424256	27.69240076588121	21.150534149782704
39	20.99147086835249	29.346467846999236	29.376504485329807	20.285556799318464
40	22.146696252814387	27.209957163296007	28.531127014294466	22.112219569595137
41	19.535313039085132	26.426528047639735	29.629834695890533	24.408324217384596
42	21.912930542838826	27.359875013620854	27.92835996416831	22.79883447937201
43	21.893312973988056	27.73437776397217	28.290586255743754	22.08172300629602
44	21.079900288266817	29.19647923898692	29.158730012722234	20.564890460024028
45	20.144731311833375	31.311072728996642	27.87005562969389	20.674140329476096
46	22.11193653884526	29.358461275025228	28.914120687142052	19.615481498987457
47	21.63713476650671	27.881482996220125	29.190305630755255	21.291076606517915
48	22.313578258709917	26.371319361992086	30.543192615161672	20.771909764136325
49	21.442391921170277	26.173144768813405	31.095137956263258	21.289325353753057
50	20.133056293400998	29.83766771340872	29.17162560126345	20.85765039192683
51	19.88230873843289	30.990788056668418	28.00817463563329	21.118728569265407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2919.0
1	4953.0
2	6987.0
3	44219.0
4	81451.0
5	58990.5
6	36530.0
7	36396.5
8	36263.0
9	37087.0
10	37911.0
11	37125.0
12	36339.0
13	34699.0
14	33059.0
15	30733.0
16	28407.0
17	27322.0
18	26237.0
19	25070.5
20	23904.0
21	23812.0
22	23720.0
23	26593.0
24	29466.0
25	33397.5
26	44935.5
27	52542.0
28	65767.5
29	78993.0
30	94028.0
31	109063.0
32	122312.0
33	135561.0
34	153319.0
35	171077.0
36	194840.5
37	218604.0
38	241313.5
39	264023.0
40	322386.0
41	380749.0
42	422823.5
43	464898.0
44	472356.5
45	479815.0
46	471364.0
47	462913.0
48	457144.5
49	451376.0
50	434313.0
51	417250.0
52	395090.0
53	372930.0
54	340098.5
55	307267.0
56	275504.0
57	243741.0
58	216514.0
59	189287.0
60	167351.5
61	145416.0
62	126495.5
63	107575.0
64	85249.0
65	62923.0
66	51183.5
67	39444.0
68	30393.5
69	21343.0
70	18523.0
71	15703.0
72	12847.5
73	9992.0
74	7975.5
75	4182.5
76	2406.0
77	1745.0
78	1084.0
79	731.0
80	378.0
81	264.0
82	150.0
83	104.5
84	59.0
85	45.0
86	31.0
87	20.0
88	9.0
89	8.5
90	8.0
91	6.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5653096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.040364836771705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.49283853315197	14.057080650258087
2	7.056924154071814	2.405051244216205
3	1.9879356578277811	1.0162544664423945
4	1.0277888209150357	0.7005558593419049
5	0.6286998028593404	0.5356637006764803
6	0.4506177008463196	0.4607214014597121
7	0.3427926819280628	0.408891865440074
8	0.25202625627337805	0.34356954842752685
9	0.2127756865309712	0.3263197794194088
>10	2.583398799734131	10.743646933956882
>50	1.2428787997642088	15.523909735857307
>100	1.711874525702373	51.424884036671095
>500	0.00808378544870857	0.8550737631389418
>1k	0.0011548264926713962	0.30017119634486256
>5k	1.0498422660649057E-4	0.10132723380995898
>10k+	1.0498422660649057E-4	0.7968785845393385
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44544	0.7879576076542836	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5664	0.10019288545604038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.30682656017163E-5	0.0	0.0	0.013213998134827359	0.0
2	5.30682656017163E-5	0.0	0.0	0.04540874593320191	0.0
3	5.30682656017163E-5	0.0	0.0	0.07107609706256536	0.0
4	5.30682656017163E-5	0.0	0.0	0.10397842173562946	0.0
5	5.30682656017163E-5	0.0	0.0	0.18984287547920645	0.0
6	5.30682656017163E-5	0.0	0.0	0.31487170923685004	0.0
7	5.30682656017163E-5	0.0	0.0	0.40959856333591366	0.0
8	5.30682656017163E-5	0.0	0.0	0.60943596217011	0.0
9	5.30682656017163E-5	0.0	0.0	0.7067631612836577	0.0
10	5.30682656017163E-5	0.0	0.0	0.8646943197143654	0.0
11	5.30682656017163E-5	0.0	0.0	0.991279822596326	0.0
12	5.30682656017163E-5	0.0	0.0	1.09580661640984	0.0
13	5.30682656017163E-5	0.0	0.0	1.140047860499804	0.0
14	5.30682656017163E-5	0.0	0.0	1.1605322110220664	0.0
15	5.30682656017163E-5	0.0	0.0	1.1873670639946676	0.0
16	5.30682656017163E-5	0.0	0.0	1.2469450368435278	0.0
17	7.075768746895507E-5	0.0	0.0	1.3186402636714465	0.0
18	7.075768746895507E-5	0.0	0.0	1.4174887530655769	0.0
19	7.075768746895507E-5	0.0	0.0	1.4688588341680382	0.0
20	7.075768746895507E-5	0.0	0.0	1.524704339002911	0.0
21	7.075768746895507E-5	0.0	0.0	1.6028385153905047	0.0
22	7.075768746895507E-5	0.0	0.0	1.6900827440397261	0.0
23	7.075768746895507E-5	0.0	0.0	1.7802103484533076	0.0
24	7.075768746895507E-5	0.0	0.0	1.8522593637185711	0.0
25	7.075768746895507E-5	0.0	0.0	1.9197975764076889	0.0
26	7.075768746895507E-5	0.0	0.0	1.9882202601901684	0.0
27	7.075768746895507E-5	0.0	0.0	2.0565544968633116	0.0
28	7.075768746895507E-5	0.0	0.0	2.131593024424139	0.0
29	7.075768746895507E-5	0.0	0.0	2.215653157137257	0.0
30	7.075768746895507E-5	0.0	0.0	2.314307062890848	0.0
31	7.075768746895507E-5	0.0	0.0	2.4061505412255513	0.0
32	7.075768746895507E-5	0.0	0.0	2.489308513423441	0.0
33	7.075768746895507E-5	0.0	0.0	2.5755621344480972	0.0
34	7.075768746895507E-5	0.0	0.0	2.6644691687528392	0.0
35	7.075768746895507E-5	0.0	0.0	2.773966690111047	0.0
36	7.075768746895507E-5	0.0	0.0	2.8721606708960894	0.0
37	7.075768746895507E-5	0.0	0.0	2.975413826335162	0.0
38	7.075768746895507E-5	0.0	0.0	3.0938975740019274	0.0
39	7.075768746895507E-5	0.0	0.0	3.243903871436112	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32170	0.0	43.566208	1
TAGTACG	260	0.0	38.94231	1
CGAGGGT	2200	0.0	38.659092	4
GGGCGAT	7965	0.0	38.615818	7
TAGGGCG	2040	0.0	38.382355	5
ATAGGGC	3015	0.0	38.35821	4
CGGGTCA	1365	0.0	38.24176	6
GCGCGAC	510	0.0	37.941177	9
AAGGGTC	4615	0.0	37.589382	5
TACGGGT	1270	0.0	37.559055	4
ACGGGAT	1795	0.0	37.35376	5
TAGGGTC	4765	0.0	37.35047	5
TCGATAG	280	0.0	36.964283	1
GCGGGTC	1825	0.0	36.863014	5
GTTTTTT	39595	0.0	36.85693	2
GGCGATA	2040	0.0	36.838234	8
ACCGCTC	245	0.0	36.734695	18
AGGGCGA	4630	0.0	36.690063	6
GCGATAT	595	0.0	36.68067	9
GTTACGG	825	0.0	36.545452	2
>>END_MODULE