FastQCFastQC Report
Thu 26 May 2016
SRR1546801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546801_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences654512
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20870.3188635196910064No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.27898648153127825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG207.031057E-445.0000042
ACGCGAG207.031057E-445.0000041
CCTTCAT302.1640626E-645.00000438
GTTTGCG406.8066583E-945.0000041
CTAGAGT207.031057E-445.00000414
CTATGCG453.8380676E-1045.0000041
TCGTTGA406.8066583E-945.00000424
GCAAGTA207.031057E-445.00000432
AGATCCC207.031057E-445.00000432
CCCTCAT207.031057E-445.00000425
AGTTTCG207.031057E-445.0000041
CCTAGAC207.031057E-445.00000414
GCGAAAA207.031057E-445.00000424
TTTGTCG207.031057E-445.0000041
GTTTACG207.031057E-445.0000041
TTAGACG302.1640626E-645.0000041
CGTTGAT406.8066583E-945.00000425
TCCCTTA207.031057E-445.00000435
GTGATTG600.045.0000041
TAAGTTG406.8066583E-945.0000041