FastQCFastQC Report
Thu 26 May 2016
SRR1546800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546800_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2627100
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151160.5753873092002588No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36460.13878421072665675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101000.042.1262361
TACGCGG2200.040.9090922
TACGGGT2150.040.8139534
CACGACG2500.040.50000426
AGGGCGA16900.040.4733736
TCGATAG900.040.01
TACGGGA5100.039.7058834
CGACCGT403.4604818E-739.37522
GGGCGAT33050.039.1452377
TCACGAC2550.038.8235325
CGATAAT1000.038.25000410
ATAACGG2300.038.1521722
GGCGATA7750.038.0322578
CGACTAT301.1403213E-437.50000413
TAACGGG9500.037.1842083
TTAGACG1150.037.1739121
TCGCCTA250.002107600236.0000048
CGAACTA250.002107600236.00000412
CAATTCG250.002107600236.00000441
GTAGCGT504.883077E-836.00000434
Produced by FastQC (version 0.11.5)