FastQCFastQC Report
Thu 26 May 2016
SRR1546799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546799_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3383440
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440171.3009540585912562No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38790.11464663183032653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA207.035232E-445.00000444
CGTTTTT239800.043.5738141
TATTACG1350.041.6666641
CCAATCG451.9301297E-840.024
CGGTCTA4400.039.37531
TACGGGT8100.038.611114
TGCGTAT356.251894E-638.57142623
GCGCGAC7900.038.4493649
CACGACG4450.038.42696426
GTTTTTT282900.038.3430562
CGTTCAC1000.038.2521
CGACGGT4650.038.22580728
CGTTAGG4550.038.0769232
GGCGATA9950.037.9899528
GTAGGGT29450.037.8183364
GCGTTAG2450.037.653061
CGAGGGT10550.037.5355454
ACGGGTA7200.037.55
GGGCGAT45050.037.008887
GCGATAA2950.036.610179
Produced by FastQC (version 0.11.5)