FastQCFastQC Report
Thu 26 May 2016
SRR1546798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546798_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2171392
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142420.6558926255600095No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25620.11798882928554587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC253.8919556E-545.045
GTATGCG502.1827873E-1145.01
ACGCGTA253.8919556E-545.045
ACGTTAG900.042.5000041
CGTTTTT96850.042.3515781
ACGGGAC4050.040.05
CGTTAGG2650.039.9056632
ATAGGGC7950.038.7735824
TATACGC301.1402563E-437.49999613
CGGTCTA1950.036.92307731
AGGGCGA15100.036.655636
ACGGGAT5700.036.315795
CGATAGT751.8189894E-1236.00000410
CGTATAA250.002107520136.020
TCGACCA250.002107520136.038
GCGTAAG1000.036.01
GGGCGAT27650.035.967457
TTTACGG2200.035.7954562
TAGGGAC12600.035.7142875
AGGGTAC6250.035.646
Produced by FastQC (version 0.11.5)