##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546798_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2171392
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648204930293563	31.0	31.0	33.0	30.0	34.0
2	32.02219728174369	33.0	31.0	34.0	30.0	34.0
3	32.07161166661754	33.0	31.0	34.0	30.0	34.0
4	35.80251746345201	37.0	35.0	37.0	35.0	37.0
5	35.75661419034426	37.0	35.0	37.0	35.0	37.0
6	35.81108017345555	37.0	35.0	37.0	35.0	37.0
7	36.0856745350448	37.0	35.0	37.0	35.0	37.0
8	35.94987224784838	37.0	35.0	37.0	35.0	37.0
9	37.69379319809597	39.0	38.0	39.0	35.0	39.0
10	37.44940664790144	39.0	37.0	39.0	35.0	39.0
11	37.20475575114949	39.0	37.0	39.0	34.0	39.0
12	36.64383261981254	39.0	35.0	39.0	33.0	39.0
13	36.43944621698892	39.0	35.0	39.0	33.0	39.0
14	37.506986301874555	40.0	35.0	41.0	33.0	41.0
15	37.665587328312895	40.0	35.0	41.0	33.0	41.0
16	37.74590585209856	40.0	35.0	41.0	33.0	41.0
17	37.64444559066258	40.0	35.0	41.0	33.0	41.0
18	37.50994200954963	39.0	35.0	41.0	33.0	41.0
19	37.44449459148785	39.0	35.0	41.0	33.0	41.0
20	37.328349280093136	39.0	35.0	41.0	33.0	41.0
21	37.20098627976892	39.0	35.0	41.0	32.0	41.0
22	37.14032196858052	39.0	35.0	41.0	32.0	41.0
23	37.02989418769158	39.0	35.0	41.0	32.0	41.0
24	37.00284103469111	39.0	35.0	41.0	32.0	41.0
25	36.90099806944117	38.0	35.0	41.0	32.0	41.0
26	36.84701242336713	38.0	35.0	41.0	32.0	41.0
27	36.75416000427376	38.0	35.0	41.0	32.0	41.0
28	36.712142257132754	38.0	35.0	41.0	32.0	41.0
29	36.63085338805706	38.0	35.0	40.0	31.0	41.0
30	36.61608636303348	38.0	35.0	41.0	31.0	41.0
31	36.492791260168595	38.0	35.0	41.0	31.0	41.0
32	36.273509343315254	38.0	35.0	41.0	30.0	41.0
33	36.14754636657038	38.0	35.0	41.0	30.0	41.0
34	35.913136365980904	38.0	35.0	41.0	30.0	41.0
35	35.76518104515444	38.0	35.0	41.0	29.0	41.0
36	35.64707155594199	38.0	35.0	41.0	28.0	41.0
37	35.393078725536434	38.0	35.0	40.0	27.0	41.0
38	35.453154474180614	38.0	35.0	40.0	27.0	41.0
39	35.35452465515208	38.0	35.0	40.0	27.0	41.0
40	35.20626307916765	38.0	34.0	40.0	26.0	41.0
41	35.15330810834709	38.0	34.0	40.0	26.0	41.0
42	35.143359651320445	38.0	34.0	40.0	26.0	41.0
43	35.06000805013558	37.0	34.0	40.0	26.0	41.0
44	34.90916379907451	37.0	34.0	40.0	26.0	41.0
45	34.88790646737208	37.0	34.0	40.0	26.0	41.0
46	34.71294634962273	37.0	34.0	40.0	24.0	41.0
47	34.58303153000472	37.0	34.0	40.0	24.0	41.0
48	34.4390496971528	36.0	34.0	40.0	23.0	41.0
49	34.46552165615421	36.0	34.0	40.0	24.0	41.0
50	34.34528542059656	36.0	34.0	40.0	24.0	41.0
51	33.7584475764855	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	7.0
12	11.0
13	16.0
14	40.0
15	67.0
16	185.0
17	412.0
18	789.0
19	1715.0
20	3088.0
21	4827.0
22	7430.0
23	11087.0
24	15879.0
25	23679.0
26	32135.0
27	37337.0
28	39200.0
29	41073.0
30	46793.0
31	56229.0
32	69573.0
33	92753.0
34	166928.0
35	268958.0
36	149768.0
37	199609.0
38	314456.0
39	587185.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.00160265857109	22.730856519688754	27.517417398608817	14.750123423131337
2	33.864037446946476	24.508379877976893	28.766017375029474	12.86156530004716
3	29.980353616481963	24.45251709502476	32.00255872730488	13.564570561188399
4	26.782543179674605	26.571710681443054	31.07614838776232	15.569597751120018
5	23.398815137939167	30.451618132515918	30.706385581230844	15.443181148314078
6	22.28192790615421	39.13480384932799	28.221435834708796	10.361832409809008
7	83.6464811512615	4.954333441405328	8.857267596085828	2.5419178112473473
8	83.99598045861825	4.498450763381278	8.897241953548692	2.60832682445178
9	79.07899633046452	6.515636052817732	10.346726892242396	4.05864072447536
10	45.30476302758784	26.16386170714454	16.300511377033718	12.230863888233907
11	37.82200542324923	24.358337877269513	23.241680912520632	14.577975786960623
12	34.075376532657394	22.85644416116482	26.962335681443054	16.105843624734735
13	24.018463732020752	31.218729736500826	27.717151025701487	17.04565550577694
14	18.329117911459562	33.63552044034426	30.017841089955198	18.01752055824098
15	17.420346026880456	25.244359378684273	40.55020926668239	16.78508532775289
16	20.964478085946713	22.357271280358407	38.21553178790379	18.462718845791088
17	21.685213908865833	21.970422659750056	29.400449112827165	26.943914318556946
18	23.723307445178023	24.22607249174723	31.54764317083235	20.502976892242394
19	27.908779253124262	25.406835799339778	27.175424796628157	19.508960150907807
20	29.933931782008962	24.442293238622966	26.69702200247583	18.926752976892242
21	26.036339822565434	25.570371448361236	29.282276069912754	19.111012659160576
22	24.87892559243103	23.652661518509785	28.816952443409573	22.65146044564961
23	22.551202178141946	27.05996890473945	28.35448412815374	22.034344788964866
24	22.47995755717991	24.40899662520632	33.479077016033955	19.631968801579816
25	21.334977746993633	25.76011148608819	32.22674671362886	20.67816405328932
26	20.38821180146192	30.810189961094082	28.071670154444707	20.729928082999294
27	20.11198346498467	29.57844553171422	30.43370335710917	19.875867646191935
28	19.28785774286725	27.374882103277532	34.44979073331761	18.88746942053761
29	20.216478645956144	25.97743751473709	33.982164436453665	19.8239194028531
30	21.299654783659513	26.778812853690166	31.844641593963686	20.07689076868663
31	25.88933734673426	26.01796451308654	27.35936210504598	20.733336035133224
32	26.89233450247583	26.826754450601275	27.715861530299456	18.56504951662344
33	26.168605208087715	26.6930153560481	27.502035560598912	19.636343875265265
34	21.874769732963923	27.560247067319025	29.559103100683803	21.00588009903325
35	22.22454536076397	27.616846704786607	29.089358347087952	21.069249587361472
36	26.140788950129686	26.816484540792267	27.57724077458147	19.465485734496582
37	23.3859662373261	29.024561203136052	27.97505010610705	19.614422453430794
38	22.419259166470173	30.369689121079936	25.65598473237444	21.555066980075456
39	22.42805536724829	28.884190417943884	27.188596071091727	21.499158143716105
40	23.55751517920302	26.73170021810894	27.406014206555056	22.304770396132987
41	20.519556118839898	26.41885942289554	28.088617808299933	24.972966649964633
42	22.868371993633577	27.121726523815138	26.668376783187924	23.341524699363358
43	23.25508245402028	27.60510308594671	26.09878824274935	23.041026217283658
44	22.00993648314077	29.591386539141713	27.272873806295685	21.125803171421833
45	20.54654341546805	31.554965662579583	25.655247877858994	22.243243044093376
46	22.610150539377504	29.678795906036314	27.32413124852629	20.38692230605989
47	23.03780247877859	27.92061497877859	27.872903648903563	21.16867889353926
48	23.414933830464513	26.1791053849328	28.436965780476303	21.968995004126384
49	22.367541190167415	26.004609024994107	29.57757051697713	22.050279267861352
50	20.74254671657628	29.86890437102099	27.449258355930205	21.93929055647253
51	20.864173765031833	30.645180603041734	25.97462825689696	22.516017375029474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1337.0
1	2573.0
2	3809.0
3	14886.0
4	25963.0
5	18663.5
6	11364.0
7	11614.0
8	11864.0
9	12369.0
10	12874.0
11	12629.5
12	12385.0
13	12191.5
14	11998.0
15	11532.0
16	11066.0
17	10297.5
18	9529.0
19	9620.0
20	9711.0
21	10259.0
22	10807.0
23	12351.5
24	13896.0
25	14956.5
26	18035.0
27	20053.0
28	23240.0
29	26427.0
30	30944.5
31	35462.0
32	41768.5
33	48075.0
34	53812.0
35	59549.0
36	65462.5
37	71376.0
38	81419.5
39	91463.0
40	108077.0
41	124691.0
42	142585.5
43	160480.0
44	164364.5
45	168249.0
46	166204.5
47	164160.0
48	156992.5
49	149825.0
50	148762.5
51	147700.0
52	139704.5
53	131709.0
54	125915.5
55	120122.0
56	111856.0
57	103590.0
58	100449.0
59	97308.0
60	88429.0
61	79550.0
62	72761.5
63	65973.0
64	57386.5
65	48800.0
66	42000.0
67	35200.0
68	28497.0
69	21794.0
70	18250.0
71	14706.0
72	12361.0
73	10016.0
74	7931.0
75	4523.5
76	3201.0
77	2625.5
78	2050.0
79	1409.0
80	768.0
81	552.0
82	336.0
83	250.0
84	164.0
85	121.5
86	79.0
87	47.5
88	16.0
89	16.5
90	17.0
91	12.0
92	7.0
93	5.5
94	4.0
95	5.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2171392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.892988451212954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.05979389166939	16.324145321958945
2	5.453068226391389	2.1695564650256043
3	1.7190882500911233	1.025934081170358
4	0.9610170203900656	0.7647000195215464
5	0.634455770880067	0.6310610661461292
6	0.4577522335936797	0.5463635937837602
7	0.3751180502869792	0.5223553329540278
8	0.3216575509498578	0.5118983957032771
9	0.28216239794517206	0.5051747991320876
>10	4.635653935586377	24.195474701733954
>50	2.4003284268352973	34.05940753642431
>100	0.6980452700154592	17.734451427502442
>500	0.001161859603277235	0.15286845385688327
>1k	4.6474384131089394E-4	0.19826211024861576
>5k	0.0	0.0
>10k+	2.3237192065544697E-4	0.6583466948381408
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14242	0.6558926255600095	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2562	0.11798882928554587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3816022164583826E-4	0.0	0.0	0.008888307592548926	0.0
2	1.3816022164583826E-4	0.0	0.0	0.03348082704550814	0.0
3	1.3816022164583826E-4	0.0	0.0	0.052823258075925486	0.0
4	1.3816022164583826E-4	0.0	0.0	0.07870527293091252	0.0
5	1.3816022164583826E-4	0.0	0.0	0.13834443527469936	0.0
6	1.3816022164583826E-4	0.0	0.0	0.22377350565904267	0.0
7	1.3816022164583826E-4	0.0	0.0	0.280695516977128	0.0
8	1.3816022164583826E-4	0.0	0.0	0.4016317643244518	0.0
9	1.3816022164583826E-4	0.0	0.0	0.4665210150907805	0.0
10	1.3816022164583826E-4	0.0	0.0	0.5780623673661872	0.0
11	1.3816022164583826E-4	0.0	0.0	0.6920445502240038	0.0
12	1.3816022164583826E-4	0.0	0.0	0.7829539760669654	0.0
13	1.3816022164583826E-4	0.0	0.0	0.8246323095967932	0.0
14	1.3816022164583826E-4	0.0	0.0	0.8438365804055648	0.0
15	1.3816022164583826E-4	0.0	0.0	0.8696725418533364	0.0
16	1.3816022164583826E-4	0.0	0.0	0.9176601921716576	0.0
17	1.3816022164583826E-4	0.0	0.0	0.9755953784484791	0.0
18	1.3816022164583826E-4	0.0	0.0	1.0511690196887526	0.0
19	1.8421362886111766E-4	0.0	0.0	1.0925710327752889	0.0
20	2.302670360763971E-4	0.0	0.0	1.1394534013204434	0.0
21	2.302670360763971E-4	0.0	0.0	1.2019939283187928	0.0
22	2.302670360763971E-4	0.0	0.0	1.2659160575336006	0.0
23	2.302670360763971E-4	0.0	0.0	1.336700144423485	0.0
24	2.302670360763971E-4	0.0	0.0	1.3913655387880217	0.0
25	2.302670360763971E-4	0.0	0.0	1.4389847618486207	0.0
26	2.302670360763971E-4	0.0	0.0	1.4902882574864418	0.0
27	2.302670360763971E-4	0.0	0.0	1.5439404768922425	0.0
28	2.302670360763971E-4	0.0	0.0	1.5982374439990568	0.0
29	2.302670360763971E-4	0.0	0.0	1.6594424221881632	0.0
30	2.302670360763971E-4	0.0	0.0	1.7372726803819853	0.0
31	2.302670360763971E-4	0.0	0.0	1.8050632958028767	0.0
32	2.302670360763971E-4	0.0	0.0	1.8750644747701013	0.0
33	2.302670360763971E-4	0.0	0.0	1.941427434567319	0.0
34	2.302670360763971E-4	0.0	0.0	2.0102312249469465	0.0
35	2.302670360763971E-4	0.0	0.0	2.0911470614241923	0.0
36	2.302670360763971E-4	0.0	0.0	2.164556192525348	0.0
37	2.302670360763971E-4	0.0	0.0	2.245656242631455	0.0
38	2.302670360763971E-4	0.0	0.0	2.3331116629332707	0.0
39	2.302670360763971E-4	0.0	0.0	2.463903339424664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	25	3.8919556E-5	45.0	45
GTATGCG	50	2.1827873E-11	45.0	1
ACGCGTA	25	3.8919556E-5	45.0	45
ACGTTAG	90	0.0	42.500004	1
CGTTTTT	9685	0.0	42.351578	1
ACGGGAC	405	0.0	40.0	5
CGTTAGG	265	0.0	39.905663	2
ATAGGGC	795	0.0	38.773582	4
TATACGC	30	1.1402563E-4	37.499996	13
CGGTCTA	195	0.0	36.923077	31
AGGGCGA	1510	0.0	36.65563	6
ACGGGAT	570	0.0	36.31579	5
CGATAGT	75	1.8189894E-12	36.000004	10
CGTATAA	25	0.0021075201	36.0	20
TCGACCA	25	0.0021075201	36.0	38
GCGTAAG	100	0.0	36.0	1
GGGCGAT	2765	0.0	35.96745	7
TTTACGG	220	0.0	35.795456	2
TAGGGAC	1260	0.0	35.714287	5
AGGGTAC	625	0.0	35.64	6
>>END_MODULE