FastQCFastQC Report
Thu 26 May 2016
SRR1546797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546797_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1900729
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347451.8279828423725843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT207.034451E-445.00000417
ATAACGC351.2124838E-745.00000411
GTACGAT207.034451E-445.00000411
CGTTTTT202700.044.0342861
CGGTCTA1750.042.4285731
GTTTTTT235000.039.0542562
GCTACGA2150.038.72093210
CAACGTA356.250444E-638.5714333
TATACGC356.250444E-638.5714312
TAGGGAC12000.038.06255
TACGGGA3800.037.8947374
GTTTACG900.037.51
CATACGA4000.037.12518
GACCGAT4250.037.0588239
AGGGCGA10400.036.7788436
CGTAAGG3050.036.147542
CGGAATA250.002107454536.023
ACATACG4250.036.017
TCGACGG250.002107454536.02
ATAGGGA16850.035.786354
Produced by FastQC (version 0.11.5)