##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546797_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1900729
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605996436104252	31.0	31.0	33.0	30.0	34.0
2	31.988504410676114	31.0	31.0	34.0	30.0	34.0
3	31.93287049337386	33.0	31.0	34.0	30.0	34.0
4	35.73837827486191	37.0	35.0	37.0	35.0	37.0
5	35.72276952684996	37.0	35.0	37.0	33.0	37.0
6	35.78782719682816	37.0	35.0	37.0	35.0	37.0
7	36.07428570827298	37.0	35.0	37.0	35.0	37.0
8	35.94207222597225	37.0	35.0	37.0	35.0	37.0
9	37.71291751743673	39.0	38.0	39.0	35.0	39.0
10	37.436005869326976	39.0	37.0	39.0	35.0	39.0
11	37.192771299853895	39.0	37.0	39.0	34.0	39.0
12	36.69277840239192	39.0	35.0	39.0	33.0	39.0
13	36.53143872693056	39.0	35.0	39.0	33.0	39.0
14	37.621570986710886	40.0	35.0	41.0	33.0	41.0
15	37.77783208442656	40.0	35.0	41.0	33.0	41.0
16	37.835156405779045	40.0	35.0	41.0	33.0	41.0
17	37.71783931323192	40.0	35.0	41.0	33.0	41.0
18	37.50571912145288	39.0	36.0	41.0	33.0	41.0
19	37.32054753728701	38.0	36.0	41.0	33.0	41.0
20	37.08297658424741	38.0	35.0	41.0	33.0	41.0
21	36.96710998779942	38.0	35.0	41.0	32.0	41.0
22	36.910436995489626	38.0	35.0	41.0	32.0	41.0
23	36.79902868846637	38.0	35.0	41.0	32.0	41.0
24	36.736818347065785	38.0	35.0	41.0	32.0	41.0
25	36.650322060640946	38.0	35.0	40.0	32.0	41.0
26	36.59220435948523	38.0	35.0	40.0	32.0	41.0
27	36.486256062805374	38.0	35.0	40.0	31.0	41.0
28	36.426313272433894	38.0	35.0	40.0	31.0	41.0
29	36.30066148304151	38.0	35.0	40.0	31.0	41.0
30	36.20278535235691	38.0	35.0	40.0	31.0	41.0
31	35.97800685947339	38.0	35.0	40.0	30.0	41.0
32	35.599500507436886	38.0	35.0	40.0	29.0	41.0
33	35.269719144601886	38.0	35.0	40.0	26.0	41.0
34	34.8314457242458	38.0	34.0	40.0	23.0	41.0
35	34.530470677303285	38.0	34.0	40.0	22.0	41.0
36	34.35202493359127	37.0	34.0	40.0	21.0	41.0
37	34.061166005253774	37.0	33.0	40.0	18.0	41.0
38	34.111447239453916	37.0	33.0	40.0	20.0	41.0
39	33.98259720349403	37.0	33.0	40.0	18.0	41.0
40	33.85828121736449	37.0	33.0	40.0	18.0	41.0
41	33.74958187095583	37.0	33.0	40.0	18.0	41.0
42	33.72461197782535	36.0	33.0	40.0	18.0	41.0
43	33.60797988561231	36.0	33.0	40.0	18.0	41.0
44	33.45589613248391	36.0	33.0	40.0	18.0	41.0
45	33.437790447770304	36.0	33.0	40.0	18.0	41.0
46	33.261338149730975	35.0	33.0	40.0	18.0	41.0
47	33.13922868541491	35.0	33.0	40.0	17.0	41.0
48	33.00580829776365	35.0	33.0	40.0	17.0	41.0
49	33.03542377687719	35.0	33.0	40.0	17.0	41.0
50	32.91456067645625	35.0	32.0	40.0	17.0	41.0
51	32.3434182358453	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	4.0
12	8.0
13	14.0
14	22.0
15	61.0
16	145.0
17	448.0
18	1011.0
19	1980.0
20	3562.0
21	5972.0
22	9192.0
23	13566.0
24	20847.0
25	32596.0
26	45495.0
27	50748.0
28	47341.0
29	43670.0
30	45579.0
31	51855.0
32	63403.0
33	81176.0
34	140386.0
35	222388.0
36	137431.0
37	175019.0
38	260425.0
39	446299.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.116639983921957	21.83825258624454	26.754682019372567	20.290425410460934
2	37.61293693104067	23.06194097106952	26.932245470027556	12.392876627862256
3	26.858589520126223	23.132282403225286	37.027529963503476	12.98159811314501
4	24.114642329337848	25.222533038639384	36.520934862360704	14.141889769662061
5	22.049276882711844	28.852140415598438	35.134203771289855	13.964378930399862
6	20.49276882711844	36.3678883207443	32.937362454089985	10.201980398047276
7	78.81817976155465	4.501220321255687	14.692152326817762	1.9884475903719045
8	79.64665136376622	3.892980009249083	14.35549202437591	2.1048766026087886
9	75.49840087671626	5.378410073187709	15.631107853881327	3.492081196214716
10	43.08036548082341	23.72700158728572	22.094996183043452	11.097636748847416
11	35.14067497260262	22.251778133547706	28.212911993240485	14.394634900609187
12	31.116376926958022	20.44420851157635	32.40225197805684	16.037162583408787
13	22.600223387973774	28.059128892125074	32.98271347467209	16.357934245229067
14	17.827107388796616	30.46052330447949	34.8674113984687	16.8449579082552
15	17.07949949729814	23.285855058769556	42.762855725355905	16.871789718576398
16	19.755683214177296	20.942227955694896	41.49571033008914	17.80637850003867
17	20.611512740637934	21.51921710038622	33.06925921580615	24.8000109431697
18	22.774367098097624	23.162376119899257	35.15656361322419	18.906693168778926
19	25.712397716876	24.32224688527402	30.976009731003213	18.989345666846773
20	26.987382209667977	23.555435835408414	31.171040164063367	18.286141790860245
21	23.929976340656665	24.67484843973023	33.311219011232005	18.0839562083811
22	23.3002705803931	22.33022172019262	33.10656069329189	21.26294700612239
23	21.442667523881624	25.530257075048574	32.23810443256245	20.788970968507346
24	21.152042190128103	23.708640211203175	36.87422036492315	18.26509723374558
25	20.138168039736332	25.01645421309403	35.10311043815294	19.742267309016697
26	19.596691585176004	28.599553118829668	32.10331404424303	19.700441251751304
27	19.7154354986955	27.229762896236124	34.34035046553191	18.71445113953646
28	18.465388806084402	26.134919812345686	37.30926397187606	18.09042740969386
29	19.283495963917	24.488604109265445	37.27433000706571	18.953569919751843
30	20.347508771634462	25.618275935180662	34.91355158994259	19.120663703242283
31	24.84715075110655	24.567258141481503	31.25969036090889	19.325900746503052
32	25.885699644715267	25.531309302904308	30.599101713079563	17.98388933930087
33	24.618501638055715	25.9107952790745	30.146696346507053	19.32400673636273
34	20.787708295080467	26.92046051804334	31.253219159596135	21.03861202728006
35	21.656743281130556	26.810870986868725	31.23091192905459	20.30147380294613
36	25.62380013142326	26.03964058000904	28.761227928863082	19.575331359704617
37	22.229260457435014	28.746286293311673	29.40271864111086	19.621734608142454
38	22.18127886721358	29.479320829008238	27.322727227290162	21.016673076488022
39	21.63585655819425	28.45392478359619	28.23222037439319	21.677998283816365
40	23.44931865615772	26.258767030965487	28.055393483237225	22.236520829639574
41	20.39043966814838	25.499058519126084	29.534825848398167	24.57567596432737
42	21.737028266523005	27.531278788296493	27.906661075829327	22.825031869351182
43	22.06842742968619	27.310573995556442	28.133942292667708	22.48705628208966
44	21.593820055357707	28.88549603862518	28.86545109797346	20.65523280804365
45	20.645710145949263	30.496404274359996	27.146742118418775	21.711143461271966
46	22.19832495847646	29.36220786866513	28.078700330241713	20.3607668426167
47	22.073530734786495	27.488242668997	29.27250544396387	21.165721152252637
48	22.92825542199861	25.56992606520972	30.31163306289324	21.190185449898433
49	21.449296559372748	25.612330847795768	30.828382162843837	22.109990429987654
50	20.142061282802544	29.197376375064515	29.487265149319025	21.173297192813916
51	19.873480122626635	30.13380655527432	28.01351481457904	21.97919850752001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1352.0
1	2345.0
2	3338.0
3	28720.5
4	54103.0
5	37375.5
6	20648.0
7	20663.5
8	20679.0
9	21466.0
10	22253.0
11	22521.0
12	22789.0
13	22014.0
14	21239.0
15	20218.5
16	19198.0
17	17935.5
18	16673.0
19	15603.0
20	14533.0
21	14063.0
22	13593.0
23	12970.0
24	12347.0
25	12905.0
26	15771.0
27	18079.0
28	20111.5
29	22144.0
30	24733.5
31	27323.0
32	32282.5
33	37242.0
34	41435.5
35	45629.0
36	49423.5
37	53218.0
38	60909.0
39	68600.0
40	83374.5
41	98149.0
42	109185.5
43	120222.0
44	123529.0
45	126836.0
46	124493.0
47	122150.0
48	124327.0
49	126504.0
50	125820.0
51	125136.0
52	120011.0
53	114886.0
54	108297.5
55	101709.0
56	96464.0
57	91219.0
58	87303.0
59	83387.0
60	78445.0
61	73503.0
62	65171.0
63	56839.0
64	50270.0
65	43701.0
66	37476.5
67	31252.0
68	26143.5
69	21035.0
70	18025.0
71	15015.0
72	12242.5
73	9470.0
74	7508.0
75	4293.0
76	3040.0
77	2169.0
78	1298.0
79	1022.0
80	746.0
81	544.0
82	342.0
83	241.0
84	140.0
85	106.5
86	73.0
87	50.0
88	27.0
89	22.0
90	17.0
91	17.5
92	18.0
93	21.0
94	24.0
95	13.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1900729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.672117221075325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54132154059484	20.419941305093943
2	6.1289591094476705	3.14686713401836
3	2.503086751816155	1.92778609521435
4	1.4160383975735487	1.454108149282072
5	0.9846319906138736	1.263879394133005
6	0.7521803068007172	1.1586036604543448
7	0.5618279839391527	1.0096319703236445
8	0.47854407927170234	0.9828191758811764
9	0.41578761677189224	0.9606733593155605
>10	5.713488174847306	35.631326470470206
>50	1.2938981616779919	22.583669686248058
>100	0.20733322344211666	7.0528731710180095
>500	0.0022806639452574123	0.38775718597535397
>1k	4.14666171864984E-4	0.1706983246977296
>5k	0.0	0.0
>10k+	2.07333085932492E-4	1.8493649178742175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34745	1.8279828423725843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013310682375025583	0.0
2	0.0	0.0	0.0	0.04424618133358306	0.0
3	0.0	0.0	0.0	0.07781224993147366	0.0
4	0.0	0.0	0.0	0.12326849329914996	0.0
5	0.0	0.0	0.0	0.2513246233418862	0.0
6	0.0	0.0	0.0	0.44609199943811034	0.0
7	0.0	0.0	0.0	0.6002959916958177	0.0
8	0.0	0.0	0.0	0.862195504987823	0.0
9	0.0	0.0	0.0	1.0455988202421282	0.0
10	0.0	0.0	0.0	1.272511757331003	0.0
11	0.0	0.0	0.0	1.4344496243283498	0.0
12	0.0	0.0	0.0	1.562821422727806	0.0
13	0.0	0.0	0.0	1.6296378915668672	0.0
14	0.0	0.0	0.0	1.665518861447371	0.0
15	0.0	0.0	0.0	1.6977170338328083	0.0
16	0.0	0.0	0.0	1.7444359506273646	0.0
17	0.0	0.0	0.0	1.7957320585943604	0.0
18	0.0	0.0	0.0	1.8674413869625812	0.0
19	0.0	0.0	0.0	1.9127924075446843	0.0
20	0.0	0.0	0.0	1.9651933547601999	0.0
21	0.0	0.0	0.0	2.0246442286091284	0.0
22	0.0	0.0	0.0	2.0923024797327763	0.0
23	0.0	0.0	0.0	2.16900989041573	0.0
24	0.0	0.0	0.0	2.227776816158432	0.0
25	0.0	0.0	0.0	2.281703493764761	0.0
26	0.0	0.0	0.0	2.338260741010423	0.0
27	0.0	0.0	0.0	2.393870983185925	0.0
28	0.0	0.0	0.0	2.4559524266741866	0.0
29	0.0	0.0	0.0	2.5262412474371674	0.0
30	0.0	0.0	0.0	2.62046825191808	0.0
31	0.0	0.0	0.0	2.695281652460714	0.0
32	0.0	0.0	0.0	2.775145746710867	0.0
33	0.0	0.0	0.0	2.852010991572181	0.0
34	0.0	0.0	0.0	2.936925779529854	0.0
35	0.0	0.0	0.0	3.0289957169065134	0.0
36	0.0	0.0	0.0	3.1125952200445197	0.0
37	0.0	0.0	0.0	3.2039812093149522	0.0
38	0.0	0.0	0.0	3.309414440459424	0.0
39	0.0	0.0	0.0	3.479191404981983	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	20	7.034451E-4	45.000004	17
ATAACGC	35	1.2124838E-7	45.000004	11
GTACGAT	20	7.034451E-4	45.000004	11
CGTTTTT	20270	0.0	44.034286	1
CGGTCTA	175	0.0	42.42857	31
GTTTTTT	23500	0.0	39.054256	2
GCTACGA	215	0.0	38.720932	10
CAACGTA	35	6.250444E-6	38.57143	33
TATACGC	35	6.250444E-6	38.57143	12
TAGGGAC	1200	0.0	38.0625	5
TACGGGA	380	0.0	37.894737	4
GTTTACG	90	0.0	37.5	1
CATACGA	400	0.0	37.125	18
GACCGAT	425	0.0	37.058823	9
AGGGCGA	1040	0.0	36.778843	6
CGTAAGG	305	0.0	36.14754	2
CGGAATA	25	0.0021074545	36.0	23
ACATACG	425	0.0	36.0	17
TCGACGG	25	0.0021074545	36.0	2
ATAGGGA	1685	0.0	35.78635	4
>>END_MODULE