FastQCFastQC Report
Thu 26 May 2016
SRR1546796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546796_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3149322
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131550.4177089544987778No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC126960.4031343889256164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC351.2128294E-745.017
TCGTTAG950.045.01
ACCGGTC253.892358E-545.08
CATATTG850.045.01
GTCGACG750.045.01
ACGTTCC502.1827873E-1145.039
ACGTTAG406.8212103E-945.01
CTATCCG207.0351595E-445.031
TTAGCCG453.8562575E-1045.01
GACACGT207.0351595E-445.09
CGTTGTA351.2128294E-745.041
ACTCTCG207.0351595E-445.016
CCGGACT207.0351595E-445.024
CCGTGAT406.8212103E-945.017
TACGGCA253.892358E-545.015
TAGCCGT406.8212103E-945.044
ATCGTAG800.045.01
GTTACTA253.892358E-545.044
CGCCTAT453.8562575E-1045.033
CTAACCG453.8562575E-1045.01