##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546796_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3149322
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65553760460188	31.0	31.0	33.0	30.0	34.0
2	32.008127781154165	33.0	31.0	34.0	30.0	34.0
3	32.04775948601	33.0	31.0	34.0	30.0	34.0
4	35.748385843048126	37.0	35.0	37.0	35.0	37.0
5	35.6592714241351	37.0	35.0	37.0	33.0	37.0
6	35.711274363180394	37.0	35.0	37.0	33.0	37.0
7	36.08839362885091	37.0	35.0	37.0	35.0	37.0
8	35.991512141343435	37.0	35.0	37.0	35.0	37.0
9	37.81879877637155	39.0	38.0	39.0	35.0	39.0
10	37.49913663956877	39.0	37.0	39.0	35.0	39.0
11	37.07727822051857	39.0	37.0	39.0	33.0	39.0
12	35.59643281950845	35.0	35.0	39.0	32.0	39.0
13	35.042191620926666	35.0	35.0	39.0	31.0	39.0
14	35.77713457055201	35.0	35.0	40.0	31.0	41.0
15	36.19247253853369	35.0	35.0	40.0	32.0	41.0
16	36.448498756240234	35.0	35.0	40.0	32.0	41.0
17	36.394296296155176	35.0	35.0	40.0	32.0	41.0
18	36.341530335735754	36.0	35.0	40.0	32.0	41.0
19	36.25871568547135	36.0	35.0	40.0	32.0	41.0
20	36.09756322154419	35.0	35.0	40.0	32.0	41.0
21	35.88789809362142	35.0	35.0	40.0	31.0	41.0
22	35.791996499564036	35.0	35.0	40.0	31.0	41.0
23	35.775938122554635	35.0	35.0	40.0	31.0	41.0
24	35.738692328062996	35.0	35.0	40.0	31.0	41.0
25	35.622044363834505	35.0	35.0	40.0	31.0	41.0
26	35.54397867223485	35.0	35.0	40.0	31.0	41.0
27	35.435021887250656	35.0	34.0	40.0	31.0	41.0
28	35.52172658114985	35.0	35.0	40.0	31.0	41.0
29	35.53117591659411	35.0	35.0	40.0	31.0	41.0
30	35.57860072739466	36.0	35.0	40.0	31.0	41.0
31	35.34258389583536	35.0	34.0	40.0	31.0	41.0
32	35.063400947886564	35.0	34.0	40.0	30.0	41.0
33	34.97082737173271	35.0	34.0	40.0	30.0	41.0
34	34.85071548733347	35.0	34.0	40.0	29.0	41.0
35	34.74278844779924	35.0	34.0	40.0	29.0	41.0
36	34.56567762839113	35.0	34.0	40.0	27.0	41.0
37	34.24816801838618	35.0	34.0	40.0	26.0	41.0
38	34.391133075627074	35.0	34.0	40.0	27.0	41.0
39	34.38150560660358	35.0	34.0	40.0	27.0	41.0
40	34.21701559891304	35.0	34.0	39.0	26.0	41.0
41	34.25564772354176	35.0	34.0	39.0	26.0	41.0
42	34.263286510556874	35.0	34.0	39.0	27.0	41.0
43	34.12218947443291	35.0	34.0	39.0	27.0	41.0
44	33.997309579649205	35.0	34.0	39.0	26.0	41.0
45	33.98825906020407	35.0	34.0	39.0	26.0	41.0
46	33.83610916889413	35.0	34.0	39.0	26.0	41.0
47	33.741611051521566	35.0	33.0	39.0	25.0	41.0
48	33.65416016526732	35.0	33.0	38.0	24.0	41.0
49	33.79383943591668	35.0	34.0	38.0	26.0	41.0
50	33.58753217359165	35.0	34.0	38.0	24.0	40.0
51	32.95487631941097	35.0	33.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	5.0
12	9.0
13	9.0
14	21.0
15	77.0
16	210.0
17	534.0
18	1370.0
19	2650.0
20	5030.0
21	8218.0
22	12489.0
23	18484.0
24	26480.0
25	37561.0
26	49191.0
27	56440.0
28	61512.0
29	68529.0
30	80615.0
31	98782.0
32	127312.0
33	174383.0
34	366046.0
35	765642.0
36	169794.0
37	188266.0
38	286405.0
39	543093.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.379039679016625	23.806552648474817	30.862960345115553	13.951447327393007
2	28.353309061442435	27.276347099470932	31.72816244258288	12.642181396503755
3	28.189623036323376	25.095306227816653	33.02872808814087	13.686342647719096
4	25.890683772570732	27.89927482804235	31.599976121844637	14.610065277542278
5	24.19200069094237	32.595428476351415	30.00595048712072	13.206620345585494
6	21.11857726837713	40.09869425863726	27.57717375358887	11.205554719396746
7	86.94649197509813	5.266339866168019	6.2253716831749815	1.5617964755588662
8	89.34710391633502	3.9175733697602215	5.397225180530921	1.33809753337385
9	86.77394690031696	4.809003334686005	6.505019175555882	1.912030589441156
10	65.3503833523533	16.955713007434618	9.718758513737242	7.975145126474842
11	61.47783554682563	15.154785696730915	14.392113604134476	8.975265152308973
12	52.84321514281487	21.014808901725516	16.976447629045236	9.165528326414384
13	21.404607086858697	51.91787311681689	17.15191396751428	9.525605828810138
14	11.828037907841752	52.91621498214536	25.27315403124863	9.982593078764253
15	10.412145852345361	22.49906487809122	57.14487118179723	9.943918087766193
16	11.480629799048812	15.423573708880834	55.657979717539206	17.437816774531154
17	13.697837185273528	15.577416345486425	27.579428207087116	43.145318262152934
18	22.051317712193292	21.50215189174051	35.35218056457866	21.094349831487538
19	31.167406825977146	23.947090834154146	23.79210509436634	21.093397245502366
20	34.51168219699351	22.481918330358088	22.004101200194835	21.00229827245356
21	21.65443863790365	30.142106777268253	26.367072023756222	21.836382561071876
22	22.909915213496745	25.55686589049961	23.795375639582105	27.73784325642154
23	18.98468940298896	31.757533843792412	23.044388601737136	26.213388151481492
24	20.63209160574879	23.647820070478662	40.1447676674535	15.575320656319041
25	14.596379792221944	24.226833585133562	39.68285872324265	21.49392789940184
26	13.793794346846719	39.77421171922084	26.647227561995884	19.784766371936563
27	15.654512304553169	38.4935233678868	29.03621795421364	16.81574637334639
28	11.67140101901298	30.234126583436055	43.34637741075698	14.748094986793983
29	11.762150710533886	24.834869219470097	42.92171457856643	20.481265491429586
30	16.208885595058238	32.71701020092579	33.47409378907587	17.600010414940105
31	32.84583157898748	26.73400814524523	21.93615006658576	18.48401020918153
32	33.74456470313293	28.591868344996165	24.029044981745276	13.634521970125633
33	29.29090769378298	29.849154833961087	23.341944710639307	17.517992761616625
34	18.532592094425404	30.623607239907507	27.9537627463943	22.890037919272782
35	19.37921876518184	27.899020805112972	30.69908380279946	22.022676626905728
36	33.578243190121555	23.50223317907791	26.641004000226083	16.278519630574454
37	20.24203939768623	32.96442853414163	29.790380278675855	17.00315178949628
38	19.95553328621208	35.427307845942714	21.864261577571302	22.752897290273907
39	19.906316343644757	33.11090450579521	27.147239945613688	19.835539204946333
40	23.874535534950063	27.377575236828754	22.924616790534596	25.82327243768659
41	16.6230382285457	24.22289622972818	26.476270130523332	32.677795411202794
42	23.988814100304765	25.247974008373863	23.015112459126122	27.748099432195243
43	24.548966412453222	27.159782327751813	23.47457643264169	24.816674827153275
44	20.12566514316415	34.14931848823334	26.165473076427244	19.55954329217527
45	15.637175239623005	43.400420788982515	19.451996334449127	21.51040763694535
46	23.482578154917153	35.55231888006371	24.11808001849287	16.84702294652627
47	23.097828675505394	29.07155889426359	24.8319797086484	22.998632721582613
48	25.04697836550216	23.461335487447773	29.880558418605656	21.61112772844441
49	22.060113256123064	21.522759501886437	32.41180165127605	24.005325590714445
50	18.785598932087606	34.95688913359765	25.85769254461754	20.39981938969721
51	17.72000449620585	38.644000200678114	20.839914114847577	22.79608118826846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3299.0
1	3799.0
2	4299.0
3	12099.0
4	19899.0
5	14697.0
6	9495.0
7	10938.0
8	12381.0
9	13970.0
10	15559.0
11	16139.5
12	16720.0
13	16438.5
14	16157.0
15	14891.5
16	13626.0
17	12946.5
18	12267.0
19	11475.5
20	10684.0
21	9982.5
22	9281.0
23	8439.0
24	7597.0
25	8828.0
26	12240.0
27	14421.0
28	15825.0
29	17229.0
30	24004.0
31	30779.0
32	34891.5
33	39004.0
34	46554.0
35	54104.0
36	57543.0
37	60982.0
38	85867.5
39	110753.0
40	185959.5
41	261166.0
42	334640.5
43	408115.0
44	421571.5
45	435028.0
46	392043.0
47	349058.0
48	312593.0
49	276128.0
50	252479.5
51	228831.0
52	210467.5
53	192104.0
54	169357.0
55	146610.0
56	129240.0
57	111870.0
58	98429.0
59	84988.0
60	73979.5
61	62971.0
62	52821.0
63	42671.0
64	33487.5
65	24304.0
66	18937.5
67	13571.0
68	11142.0
69	8713.0
70	7020.5
71	5328.0
72	4728.5
73	4129.0
74	3240.0
75	1817.5
76	1284.0
77	940.0
78	596.0
79	474.0
80	352.0
81	235.5
82	119.0
83	239.5
84	360.0
85	198.0
86	36.0
87	31.5
88	27.0
89	18.0
90	9.0
91	8.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3149322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.520006781140546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91835263859745	12.558533816697548
2	9.283560951119275	2.881618578290055
3	3.0203154431013886	1.406259484743512
4	1.340934612360225	0.8324525710758707
5	0.7532552900916449	0.584526360507616
6	0.4516778031619806	0.4206025540778762
7	0.32743451874953694	0.35572501659606137
8	0.2384565025697756	0.2960677229511981
9	0.19628538974234364	0.27417155218559897
>10	1.433837855330372	4.799400568323372
>50	0.4261893412402052	4.812377817405427
>100	1.5042401248265231	59.32628246559677
>500	0.09845635725433248	9.281456676235276
>1k	0.006591220569327696	1.3441346859922114
>5k	0.0	0.0
>10k+	4.11951285582981E-4	0.8263901293215843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13155	0.4177089544987778	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12696	0.4031343889256164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.350573234493011E-5	0.0	0.0	0.03959582411706393	0.0
2	6.350573234493011E-5	0.0	0.0	0.16479737543509365	0.0
3	6.350573234493011E-5	0.0	0.0	0.23024003261654413	0.0
4	6.350573234493011E-5	0.0	0.0	0.3557591125962985	0.0
5	6.350573234493011E-5	0.0	0.0	0.6013675324403157	0.0
6	6.350573234493011E-5	0.0	0.0	0.8515801178793404	0.0
7	6.350573234493011E-5	0.0	0.0	1.0435896996242366	0.0
8	6.350573234493011E-5	0.0	0.0	1.433356131891245	0.0
9	6.350573234493011E-5	0.0	0.0	1.5824993443033135	0.0
10	6.350573234493011E-5	0.0	0.0	1.878912350023275	0.0
11	6.350573234493011E-5	0.0	0.0	2.142334127790045	0.0
12	6.350573234493011E-5	0.0	0.0	2.4425257245845295	0.0
13	6.350573234493011E-5	0.0	0.0	2.5381018517636496	0.0
14	6.350573234493011E-5	0.0	0.0	2.5851913522974153	0.0
15	6.350573234493011E-5	0.0	0.0	2.639075966192088	0.0
16	6.350573234493011E-5	0.0	0.0	2.7673892983950195	0.0
17	6.350573234493011E-5	0.0	0.0	2.912976189795772	0.0
18	6.350573234493011E-5	0.0	0.0	3.1471535778177016	0.0
19	6.350573234493011E-5	0.0	0.0	3.243333009454098	0.0
20	6.350573234493011E-5	0.0	0.0	3.342846492038604	0.0
21	6.350573234493011E-5	0.0	0.0	3.4824638445989327	0.0
22	6.350573234493011E-5	0.0	0.0	3.617604043028944	0.0
23	6.350573234493011E-5	0.0	0.0	3.774431449054749	0.0
24	6.350573234493011E-5	0.0	0.0	3.8902659048519013	0.0
25	6.350573234493011E-5	0.0	0.0	3.9753318333279353	0.0
26	6.350573234493011E-5	0.0	0.0	4.07586140762996	0.0
27	6.350573234493011E-5	0.0	0.0	4.163404059667446	0.0
28	6.350573234493011E-5	0.0	0.0	4.251454757563692	0.0
29	6.350573234493011E-5	0.0	0.0	4.350396688557092	0.0
30	6.350573234493011E-5	0.0	0.0	4.4978252461958474	0.0
31	6.350573234493011E-5	0.0	0.0	4.6421102700835295	0.0
32	6.350573234493011E-5	0.0	0.0	4.750070015069911	0.0
33	6.350573234493011E-5	0.0	0.0	4.860538236483916	0.0
34	6.350573234493011E-5	0.0	0.0	4.993169958486303	0.0
35	6.350573234493011E-5	0.0	0.0	5.172097359368143	0.0
36	6.350573234493011E-5	0.0	0.0	5.309841292824297	0.0
37	6.350573234493011E-5	0.0	0.0	5.442155486164959	0.0
38	6.350573234493011E-5	0.0	0.0	5.569325715185681	0.0
39	6.350573234493011E-5	0.0	0.0	5.707069648641835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	35	1.2128294E-7	45.0	17
TCGTTAG	95	0.0	45.0	1
ACCGGTC	25	3.892358E-5	45.0	8
CATATTG	85	0.0	45.0	1
GTCGACG	75	0.0	45.0	1
ACGTTCC	50	2.1827873E-11	45.0	39
ACGTTAG	40	6.8212103E-9	45.0	1
CTATCCG	20	7.0351595E-4	45.0	31
TTAGCCG	45	3.8562575E-10	45.0	1
GACACGT	20	7.0351595E-4	45.0	9
CGTTGTA	35	1.2128294E-7	45.0	41
ACTCTCG	20	7.0351595E-4	45.0	16
CCGGACT	20	7.0351595E-4	45.0	24
CCGTGAT	40	6.8212103E-9	45.0	17
TACGGCA	25	3.892358E-5	45.0	15
TAGCCGT	40	6.8212103E-9	45.0	44
ATCGTAG	80	0.0	45.0	1
GTTACTA	25	3.892358E-5	45.0	44
CGCCTAT	45	3.8562575E-10	45.0	33
CTAACCG	45	3.8562575E-10	45.0	1
>>END_MODULE