FastQCFastQC Report
Thu 26 May 2016
SRR1546795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546795_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences167798
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.8152659745646551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG207.0160505E-445.0000041
GTCTCGC207.0160505E-445.00000431
AAGACGG207.0160505E-445.0000042
TACGGGA406.7557266E-945.0000044
ACGGGCG207.0160505E-445.0000045
ACGGGAC351.2036435E-745.0000045
CGGGATG207.0160505E-445.0000046
GAACGGG406.7557266E-945.0000043
CGGTCTA207.0160505E-445.00000431
GTGACGG207.0160505E-445.0000042
CGTTTAC207.0160505E-445.00000433
ACGACGG207.0160505E-445.00000427
TTTAGCG253.8765273E-545.01
TAGACGG253.8765273E-545.02
ACACAAC253.8765273E-545.011
TACGAGG253.8765273E-545.02
ATACGGG253.8765273E-545.03
GCGTAGG253.8765273E-545.02
GTACAAG302.154391E-644.9999961
CGTTTTT8550.043.4210551