##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546795_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	167798
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.575429981287023	31.0	31.0	33.0	30.0	34.0
2	31.957234293614942	31.0	31.0	34.0	30.0	34.0
3	32.00654358216427	33.0	31.0	34.0	30.0	34.0
4	35.7484534976579	37.0	35.0	37.0	35.0	37.0
5	35.70000238381864	37.0	35.0	37.0	33.0	37.0
6	35.75701736611879	37.0	35.0	37.0	33.0	37.0
7	36.03821261278442	37.0	35.0	37.0	35.0	37.0
8	35.90145889700712	37.0	35.0	37.0	35.0	37.0
9	37.667183160705136	39.0	38.0	39.0	35.0	39.0
10	37.389098797363495	39.0	37.0	39.0	35.0	39.0
11	37.09723596228799	39.0	37.0	39.0	33.0	39.0
12	36.55761093695992	39.0	35.0	39.0	33.0	39.0
13	36.3601413604453	39.0	35.0	39.0	32.0	39.0
14	37.42695383735205	40.0	35.0	41.0	33.0	41.0
15	37.58371971060442	40.0	35.0	41.0	33.0	41.0
16	37.64526990786541	40.0	35.0	41.0	33.0	41.0
17	37.51119798805707	39.0	35.0	41.0	33.0	41.0
18	37.38835981358538	39.0	35.0	41.0	32.0	41.0
19	37.313376798293184	39.0	35.0	41.0	32.0	41.0
20	37.18266010321935	39.0	35.0	41.0	32.0	41.0
21	37.06391613726028	39.0	35.0	41.0	32.0	41.0
22	37.02039356845731	39.0	35.0	41.0	32.0	41.0
23	36.867692105984574	38.0	35.0	41.0	32.0	41.0
24	36.862698005935705	38.0	35.0	41.0	32.0	41.0
25	36.775086711403	38.0	35.0	41.0	32.0	41.0
26	36.71189763882764	38.0	35.0	41.0	32.0	41.0
27	36.59218822631974	38.0	35.0	41.0	31.0	41.0
28	36.530423485381235	38.0	35.0	40.0	31.0	41.0
29	36.458318931095725	38.0	35.0	40.0	31.0	41.0
30	36.42992169155771	38.0	35.0	40.0	31.0	41.0
31	36.28500339694156	38.0	35.0	41.0	30.0	41.0
32	36.06597814038308	38.0	35.0	41.0	30.0	41.0
33	35.93110764133065	38.0	35.0	41.0	30.0	41.0
34	35.69659352316476	38.0	35.0	41.0	29.0	41.0
35	35.56838579720855	38.0	35.0	41.0	28.0	41.0
36	35.456584703035794	38.0	35.0	40.0	27.0	41.0
37	35.18101526835839	38.0	34.0	40.0	26.0	41.0
38	35.276701748530975	38.0	34.0	40.0	27.0	41.0
39	35.15868484725682	38.0	34.0	40.0	26.0	41.0
40	35.03665717112242	38.0	34.0	40.0	25.0	41.0
41	34.95427239895589	37.0	34.0	40.0	25.0	41.0
42	34.95673965124733	37.0	34.0	40.0	25.0	41.0
43	34.86201861762357	37.0	34.0	40.0	25.0	41.0
44	34.72447824169537	37.0	34.0	40.0	24.0	41.0
45	34.71078320361387	37.0	34.0	40.0	24.0	41.0
46	34.53808150275927	37.0	34.0	40.0	24.0	41.0
47	34.395588743608386	36.0	34.0	40.0	23.0	41.0
48	34.259436942037446	36.0	33.0	40.0	23.0	41.0
49	34.34529017032384	36.0	34.0	40.0	24.0	41.0
50	34.20932907424403	36.0	34.0	40.0	24.0	41.0
51	33.61355916041907	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	6.0
16	11.0
17	29.0
18	61.0
19	148.0
20	276.0
21	448.0
22	729.0
23	1052.0
24	1325.0
25	1957.0
26	2632.0
27	3054.0
28	3182.0
29	3331.0
30	3774.0
31	4397.0
32	5660.0
33	7268.0
34	12920.0
35	20745.0
36	11806.0
37	15272.0
38	23922.0
39	43776.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.29300706802226	23.231504547134055	28.308442293710296	15.167046091133388
2	32.904444629852556	24.702916602104914	29.46936197094125	12.923276797101277
3	28.16660508468516	25.15703405284926	33.3138654811142	13.362495381351385
4	25.989582712547225	27.186855624024126	31.98488658982825	14.838675073600399
5	24.039023111121704	30.80906804610305	30.64935219728483	14.502556645490412
6	21.483569530030156	39.704883252482155	28.033707195556563	10.777840021931132
7	85.5361804073946	4.309944099452913	8.352900511329098	1.800974981823383
8	86.47421304187178	3.587647051812298	8.034064768352424	1.9040751379635037
9	82.07845147141205	5.185997449314056	9.485810319550888	3.249740759723
10	46.88196522008606	28.428825134983732	14.715908413687886	9.973301231242328
11	36.87886625585525	22.79645764550233	25.110549589387237	15.214126509255177
12	33.14759413103851	22.01039344926638	28.424653452365344	16.417358967329765
13	24.033659519183782	30.450303340921824	28.533117200443392	16.98291993945101
14	18.003194316976366	33.34664298740152	30.668422746397457	17.98173994922466
15	17.59198560173542	24.07180061740903	41.512413735562994	16.823800045292554
16	21.230288799628124	22.4025316153947	37.93072623034839	18.436453354628778
17	21.749961262947114	22.009797494606612	29.507503069166496	26.732738173279774
18	24.02233638064816	24.43712082384772	32.086198881989056	19.45434391351506
19	28.170180812643775	24.71900737791869	27.71367954326035	19.397132266177188
20	28.387704263459636	25.716039523713036	27.212481674394212	18.683774538433116
21	25.885886601747337	25.219609292125057	30.00214543677517	18.892358669352436
22	24.64808877340612	23.471674275021158	29.738137522497286	22.142099429075436
23	21.94185866339289	26.35967055626408	29.654703870129563	22.04376691021347
24	21.47641807411292	24.912096687684002	34.29123112313615	19.320254115066927
25	21.597992824705898	26.18445988629185	31.974755360612168	20.242791928390087
26	20.103934492663797	29.860308227750032	28.745873013981093	21.28988426560507
27	19.93885505190765	28.604631759615728	31.317417370886425	20.139095817590196
28	18.55147260396429	27.016412591330052	35.08265891131003	19.349455893395632
29	19.64147367668268	26.134995649530985	34.44677528933598	19.77675538445035
30	21.797637635728673	26.297691271648056	32.00276522962133	19.901905863001943
31	26.63798138237643	25.22497288406298	28.00152564392901	20.13552008963158
32	27.76910332661891	25.67313078820963	28.320361386905684	18.23740449826577
33	25.96693643547599	26.48780080811452	27.65527598660294	19.88998676980655
34	21.581902048892122	27.642760938747777	29.790581532557002	20.9847554798031
35	22.08965541901572	27.077795921286306	29.94195401613845	20.890594643559517
36	26.19816684346655	27.111765336893168	27.283996233566548	19.406071586073733
37	22.12839247190074	29.858520363770722	27.89544571449004	20.117641449838498
38	22.844729972943657	30.18689138130371	25.633201826005074	21.335176819747552
39	22.2541389051121	29.064112802298002	27.185067760044817	21.496680532545085
40	23.60457216414975	26.78339431936018	26.599244329491412	23.012789186998653
41	20.631950321219563	25.750008939319898	27.600448157904147	26.017592581556393
42	22.780962824348325	26.875767291624452	26.573618279121327	23.7696516049059
43	22.69216558004267	26.96218071729103	26.863252243769292	23.482401458897005
44	21.192147701402877	29.17019273173697	27.786386011752224	21.851273555107927
45	20.673071192743656	30.5581711343401	26.29649936232851	22.47225831058773
46	22.90194162028153	29.21608124053922	27.680306082313255	20.201671056865994
47	22.748781272720773	27.40139930154114	28.379956852882632	21.469862572855458
48	23.182040310373186	25.691605382662488	29.565906625823906	21.560447681140417
49	22.24519958521556	24.87633940809783	30.260789759115127	22.617671247571487
50	20.819080084387178	29.175556323674893	28.381744716861938	21.623618875075984
51	20.49190097617373	29.873419230264965	27.371005613892898	22.26367417966841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	219.0
2	323.0
3	1366.5
4	2410.0
5	1691.5
6	973.0
7	952.0
8	931.0
9	975.5
10	1020.0
11	970.0
12	920.0
13	901.0
14	882.0
15	829.5
16	777.0
17	732.0
18	687.0
19	735.5
20	784.0
21	743.5
22	703.0
23	819.0
24	935.0
25	1053.0
26	1305.5
27	1440.0
28	1852.0
29	2264.0
30	2569.5
31	2875.0
32	3265.5
33	3656.0
34	4104.0
35	4552.0
36	5013.5
37	5475.0
38	6165.5
39	6856.0
40	8397.0
41	9938.0
42	11190.5
43	12443.0
44	12754.5
45	13066.0
46	12658.0
47	12250.0
48	12050.5
49	11851.0
50	11744.5
51	11638.0
52	11165.5
53	10693.0
54	9938.5
55	9184.0
56	8728.5
57	8273.0
58	7794.0
59	7315.0
60	6731.0
61	6147.0
62	5441.0
63	4735.0
64	4220.5
65	3706.0
66	3011.0
67	2316.0
68	1992.0
69	1668.0
70	1407.5
71	1147.0
72	966.0
73	785.0
74	601.5
75	333.5
76	249.0
77	196.0
78	143.0
79	93.5
80	44.0
81	34.0
82	24.0
83	15.0
84	6.0
85	5.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.036257881500376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.93240331178406	26.454427347167425
2	10.038845412271748	8.439909891655443
3	7.051718271520925	8.89283543308025
4	5.241295225133266	8.81297750867114
5	3.972439605307928	8.34932478337048
6	3.046671203357151	7.684239383067736
7	2.272598389474878	6.6872072372733875
8	1.6714869003062267	5.62104435094578
9	1.2050584098899852	4.559053147236558
>10	2.557559260519451	13.393485023659398
>50	0.007088578881705796	0.1907054911262351
>100	0.001417715776341159	0.09952442818150396
>500	0.0	0.0
>1k	0.001417715776341159	0.8152659745646551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1368	0.8152659745646551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00893931989654227	0.0
2	0.0	0.0	0.0	0.027413914349396298	0.0
3	0.0	0.0	0.0	0.04767637278155878	0.0
4	0.0	0.0	0.0	0.0816457883884194	0.0
5	0.0	0.0	0.0	0.1632915767768388	0.0
6	0.0	0.0	0.0	0.2806946447514273	0.0
7	0.0	0.0	0.0	0.35697684120192136	0.0
8	0.0	0.0	0.0	0.5071574154638315	0.0
9	0.0	0.0	0.0	0.6144292542223387	0.0
10	0.0	0.0	0.0	0.7640138738244794	0.0
11	0.0	0.0	0.0	0.8814169417990679	0.0
12	0.0	0.0	0.0	0.9904766445368837	0.0
13	0.0	0.0	0.0	1.038748971978212	0.0
14	0.0	0.0	0.0	1.0661628863276083	0.0
15	0.0	0.0	0.0	1.0852334354402318	0.0
16	0.0	0.0	0.0	1.1346976722010989	0.0
17	0.0	0.0	0.0	1.1847578636217357	0.0
18	0.0	0.0	0.0	1.262827924051538	0.0
19	0.0	0.0	0.0	1.3051407048951715	0.0
20	0.0	0.0	0.0	1.3552008963158082	0.0
21	0.0	0.0	0.0	1.4106246796743704	0.0
22	0.0	0.0	0.0	1.4684322816720103	0.0
23	0.0	0.0	0.0	1.5280277476489588	0.0
24	0.0	0.0	0.0	1.5697445738328228	0.0
25	0.0	0.0	0.0	1.620400719913229	0.0
26	0.0	0.0	0.0	1.6633094554166319	0.0
27	0.0	0.0	0.0	1.7240968307131193	0.0
28	0.0	0.0	0.0	1.7735610674739866	0.0
29	0.0	0.0	0.0	1.830772714811857	0.0
30	0.0	0.0	0.0	1.909438729901429	0.0
31	0.0	0.0	0.0	1.972013969177225	0.0
32	0.0	0.0	0.0	2.0304175258346344	0.0
33	0.0	0.0	0.0	2.097164447728817	0.0
34	0.0	0.0	0.0	2.169870916220694	0.0
35	0.0	0.0	0.0	2.2556883872274995	0.0
36	0.0	0.0	0.0	2.3117081252458314	0.0
37	0.0	0.0	0.0	2.392757958974481	0.0
38	0.0	0.0	0.0	2.4791713846410564	0.0
39	0.0	0.0	0.0	2.6233924123052716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	20	7.0160505E-4	45.000004	1
GTCTCGC	20	7.0160505E-4	45.000004	31
AAGACGG	20	7.0160505E-4	45.000004	2
TACGGGA	40	6.7557266E-9	45.000004	4
ACGGGCG	20	7.0160505E-4	45.000004	5
ACGGGAC	35	1.2036435E-7	45.000004	5
CGGGATG	20	7.0160505E-4	45.000004	6
GAACGGG	40	6.7557266E-9	45.000004	3
CGGTCTA	20	7.0160505E-4	45.000004	31
GTGACGG	20	7.0160505E-4	45.000004	2
CGTTTAC	20	7.0160505E-4	45.000004	33
ACGACGG	20	7.0160505E-4	45.000004	27
TTTAGCG	25	3.8765273E-5	45.0	1
TAGACGG	25	3.8765273E-5	45.0	2
ACACAAC	25	3.8765273E-5	45.0	11
TACGAGG	25	3.8765273E-5	45.0	2
ATACGGG	25	3.8765273E-5	45.0	3
GCGTAGG	25	3.8765273E-5	45.0	2
GTACAAG	30	2.154391E-6	44.999996	1
CGTTTTT	855	0.0	43.421055	1
>>END_MODULE