##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546794_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	853977
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.587507626083607	31.0	31.0	33.0	30.0	34.0
2	31.95533134967335	31.0	31.0	34.0	30.0	34.0
3	32.02045488344534	33.0	31.0	34.0	30.0	34.0
4	35.72932994682527	37.0	35.0	37.0	33.0	37.0
5	35.65976835441704	37.0	35.0	37.0	33.0	37.0
6	35.71416092002478	37.0	35.0	37.0	33.0	37.0
7	36.043775183640776	37.0	35.0	37.0	35.0	37.0
8	35.92506004260068	37.0	35.0	37.0	35.0	37.0
9	37.70746167636833	39.0	38.0	39.0	35.0	39.0
10	37.40086793906627	39.0	37.0	39.0	35.0	39.0
11	37.041344204820504	39.0	37.0	39.0	33.0	39.0
12	36.01180008360881	37.0	35.0	39.0	32.0	39.0
13	35.64288382474001	37.0	35.0	39.0	31.0	39.0
14	36.5118685866247	38.0	35.0	41.0	31.0	41.0
15	36.811743173410996	38.0	35.0	41.0	32.0	41.0
16	36.97844087135836	38.0	35.0	41.0	33.0	41.0
17	36.89291163579347	38.0	35.0	41.0	32.0	41.0
18	36.80112696243576	38.0	35.0	41.0	32.0	41.0
19	36.76212357007273	37.0	35.0	40.0	32.0	41.0
20	36.63336717499418	37.0	35.0	40.0	32.0	41.0
21	36.470886218247095	37.0	35.0	40.0	32.0	41.0
22	36.39408906797256	37.0	35.0	40.0	31.0	41.0
23	36.32773833487319	37.0	35.0	40.0	31.0	41.0
24	36.319943042962514	37.0	35.0	40.0	31.0	41.0
25	36.19970444168871	37.0	35.0	40.0	31.0	41.0
26	36.12453262792792	37.0	35.0	40.0	31.0	41.0
27	36.01761522851318	36.0	35.0	40.0	31.0	41.0
28	36.03879378484432	36.0	35.0	40.0	31.0	41.0
29	35.987463362596415	36.0	35.0	40.0	31.0	41.0
30	36.044741251813576	36.0	35.0	40.0	31.0	41.0
31	35.88151905730482	36.0	35.0	40.0	31.0	41.0
32	35.67583201889512	36.0	35.0	40.0	30.0	41.0
33	35.604001044524615	36.0	35.0	40.0	30.0	41.0
34	35.44079290191656	36.0	35.0	40.0	29.0	41.0
35	35.33185671276861	36.0	34.0	40.0	29.0	41.0
36	35.18083508103848	36.0	34.0	40.0	29.0	41.0
37	34.87932227682947	36.0	34.0	40.0	27.0	41.0
38	34.98353351436865	35.0	34.0	40.0	27.0	41.0
39	34.907053702851485	36.0	34.0	40.0	27.0	41.0
40	34.78379979788683	36.0	34.0	40.0	27.0	41.0
41	34.762611873621886	36.0	34.0	40.0	27.0	41.0
42	34.78410425573522	36.0	34.0	40.0	27.0	41.0
43	34.66748753186561	35.0	34.0	40.0	27.0	41.0
44	34.56491568274087	35.0	34.0	40.0	27.0	41.0
45	34.53962694545638	35.0	34.0	40.0	27.0	41.0
46	34.39911028048765	35.0	34.0	40.0	26.0	41.0
47	34.28046305696758	35.0	34.0	40.0	26.0	41.0
48	34.17664058868096	35.0	34.0	40.0	25.0	41.0
49	34.26218973110517	35.0	34.0	39.0	26.0	41.0
50	34.10315383201187	35.0	34.0	39.0	26.0	41.0
51	33.48797098750903	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	11.0
15	30.0
16	58.0
17	156.0
18	386.0
19	772.0
20	1400.0
21	2265.0
22	3186.0
23	4725.0
24	6852.0
25	9258.0
26	12201.0
27	14345.0
28	15665.0
29	17483.0
30	20492.0
31	25161.0
32	31732.0
33	42804.0
34	84456.0
35	153943.0
36	53122.0
37	67155.0
38	101864.0
39	184403.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.98089175703795	23.235871692094754	28.927359870347797	13.855876680519499
2	31.632350754177217	25.097631435038647	29.895770026593222	13.37424778419091
3	29.267767164689445	24.96308448588194	31.827437975495826	13.941710373932786
4	26.54954407437203	27.546058032007885	30.907155579131523	14.997242314488563
5	23.991161354462708	31.638322811972685	29.30289691642749	15.067618917137112
6	21.854218556237463	39.58350166339375	27.394297504499537	11.167982275869257
7	86.37410609419224	4.758090674573203	6.760837821159117	2.106965410075447
8	87.93117379039482	3.751154890588388	6.250753825922713	2.0669174930940764
9	84.19020652781046	4.796499203140132	7.7934183239127055	3.2198759451366956
10	57.370397563400424	19.705565840766205	12.693316096335147	10.230720499498231
11	50.3765323890456	19.843157368406878	17.88561050239058	11.894699740156936
12	43.62986356775417	21.545545137632512	21.856209242169285	12.968382052444035
13	22.772041869980107	40.78880344552605	22.688198862498638	13.750955821995205
14	15.699837349249455	42.225961589129454	27.77428431913272	14.29991674248838
15	14.37076174182677	23.23481779954261	48.406924308265914	13.987496150364706
16	17.363113994873398	18.646169627519242	45.888706604510425	18.102009773096935
17	18.669706561183734	18.42918486095059	27.906137987322843	34.994970590542835
18	23.634008878459255	22.737848911621743	32.684721017076576	20.94342119284243
19	30.145191263933334	24.148074245559307	25.25758890461921	20.449145585888147
20	32.10472881588146	23.936124743406438	23.846192578957044	20.11295386175506
21	24.59410499346001	26.74123541968929	27.653906369843682	21.010753217007018
22	23.88061973566033	24.178988427088786	26.97086689688364	24.969524940367247
23	20.582521543320254	29.384397940459756	26.018616426437713	24.01446408978228
24	21.918857299435466	24.251121517324236	35.99113325066131	17.83888793257898
25	18.704016618714554	25.061681989093383	34.97248754943049	21.261813842761573
26	17.544032216324325	34.14494769765462	27.158342671992337	21.152677414028716
27	18.768186965222718	32.4704295314745	29.542247624936035	19.219135878366746
28	15.913777537334145	28.159072199836764	38.218945006715636	17.708205256113455
29	16.428779697813876	25.38054303570237	37.799612870135846	20.39106439634791
30	19.3882270833992	28.595852113113118	32.324758160934074	19.691162642553607
31	29.107575496763964	25.885123369833146	25.063672675025206	19.943628458377685
32	30.653635870755302	26.66359866834821	25.598581694823164	17.084183766073323
33	27.80156842631593	26.790299972950095	25.422347440270638	19.98578416046334
34	20.293989182378446	28.03705486213329	29.186851636519485	22.48210431896878
35	20.72210375689275	26.65551882544846	30.86804445552983	21.754332962128956
36	29.942141298887442	24.116223270650146	27.18995944855658	18.751675981905834
37	22.25411223018887	29.9889809678715	28.47769904810083	19.279207753838804
38	21.81136025911705	32.33787326824961	23.38458764111914	22.466178831514195
39	21.30467213988199	30.268145395016493	27.157874275302497	21.269308189799023
40	23.688108696135846	26.205389606511652	24.888492313024823	25.218009384327683
41	18.633054520203707	24.589303927389146	26.614065718397566	30.16357583400958
42	23.23165612188619	26.85283093104381	24.135661733278532	25.77985121379147
43	23.27357762562692	27.487976842467653	25.174214293827585	24.06423123807784
44	20.82433133445046	31.749567025809828	26.325299159110845	21.100802480628868
45	18.58797133880655	36.043008184061165	22.858578158428156	22.510442318704133
46	23.556606325463097	31.179762452618746	25.99098102173712	19.272650200181037
47	22.810450398547033	27.662220411088356	27.092767135414654	22.434562054949957
48	25.0134371300398	23.804856571078613	29.14270524850201	22.03900105037958
49	21.733840606948434	23.125915569154675	31.296041930871677	23.84420189302522
50	19.976767524183906	32.14887520389894	26.398954538588278	21.47540273332888
51	19.510244421102676	34.1435425075851	23.727454018082454	22.61875905322977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	517.0
1	841.0
2	1165.0
3	3910.5
4	6656.0
5	4782.5
6	2909.0
7	2982.0
8	3055.0
9	3277.5
10	3500.0
11	3528.5
12	3557.0
13	3476.5
14	3396.0
15	3255.0
16	3114.0
17	3077.5
18	3041.0
19	2842.0
20	2643.0
21	2878.5
22	3114.0
23	3308.0
24	3502.0
25	4241.0
26	5602.0
27	6224.0
28	7372.0
29	8520.0
30	9737.5
31	10955.0
32	12501.0
33	14047.0
34	15388.5
35	16730.0
36	19878.5
37	23027.0
38	27406.5
39	31786.0
40	45584.5
41	59383.0
42	72890.5
43	86398.0
44	86217.0
45	86036.0
46	81026.5
47	76017.0
48	70889.5
49	65762.0
50	64289.0
51	62816.0
52	58900.5
53	54985.0
54	49613.5
55	44242.0
56	39919.0
57	35596.0
58	33732.0
59	31868.0
60	29079.5
61	26291.0
62	23816.0
63	21341.0
64	18859.5
65	16378.0
66	13504.0
67	10630.0
68	8858.5
69	7087.0
70	6240.0
71	5393.0
72	4326.5
73	3260.0
74	2642.0
75	1542.5
76	1061.0
77	805.0
78	549.0
79	374.0
80	199.0
81	156.0
82	113.0
83	88.0
84	63.0
85	38.0
86	13.0
87	13.5
88	14.0
89	13.5
90	13.0
91	8.0
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.477977187501544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85372147136871	17.275161980080235
2	4.834271172809787	2.1732927428122943
3	1.9529154056168607	1.3169276380972836
4	1.2158144389866934	1.0931619689511152
5	0.9222513144791351	1.0365172004002654
6	0.7340458335354949	0.9899919300474844
7	0.6642244610866102	1.045129559857973
8	0.6010650919711287	1.0808581940424438
9	0.5319143117434593	1.0760721988567554
>10	10.753956948492831	58.490545481889164
>50	0.8917809828547849	12.46576075650699
>100	0.042990029743721556	1.3307056131294033
>500	0.0	0.0
>1k	0.0010485373108224771	0.6258747353285742
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3994	0.467694094805832	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1317	0.1542196101300152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3419834492029645E-4	0.0	0.0	0.014637396557518528	0.0
2	2.3419834492029645E-4	0.0	0.0	0.04754226401882018	0.0
3	2.3419834492029645E-4	0.0	0.0	0.06932271009640775	0.0
4	2.3419834492029645E-4	0.0	0.0	0.1083167345256371	0.0
5	2.3419834492029645E-4	0.0	0.0	0.20269866752851656	0.0
6	2.3419834492029645E-4	0.0	0.0	0.32740928619857446	0.0
7	2.3419834492029645E-4	0.0	0.0	0.42261091340867496	0.0
8	2.3419834492029645E-4	0.0	0.0	0.6445138452206558	0.0
9	2.3419834492029645E-4	0.0	0.0	0.7509569929869305	0.0
10	2.3419834492029645E-4	0.0	0.0	0.9034201155300435	0.0
11	2.3419834492029645E-4	0.0	0.0	1.0290675275797827	0.0
12	2.3419834492029645E-4	0.0	0.0	1.1433563199008872	0.0
13	2.3419834492029645E-4	0.0	0.0	1.1832871377097978	0.0
14	2.3419834492029645E-4	0.0	0.0	1.200032319371599	0.0
15	2.3419834492029645E-4	0.0	0.0	1.2277848232446542	0.0
16	2.3419834492029645E-4	0.0	0.0	1.2834069301632245	0.0
17	2.3419834492029645E-4	0.0	0.0	1.3428933097729798	0.0
18	2.3419834492029645E-4	0.0	0.0	1.4325912758774533	0.0
19	2.3419834492029645E-4	0.0	0.0	1.4784941514818315	0.0
20	2.3419834492029645E-4	0.0	0.0	1.5280271014324742	0.0
21	2.3419834492029645E-4	0.0	0.0	1.5968814148390413	0.0
22	2.3419834492029645E-4	0.0	0.0	1.6687803067295723	0.0
23	2.3419834492029645E-4	0.0	0.0	1.7527404133834985	0.0
24	2.3419834492029645E-4	0.0	0.0	1.813046487200475	0.0
25	2.3419834492029645E-4	0.0	0.0	1.8696053874987266	0.0
26	2.3419834492029645E-4	0.0	0.0	1.9242907010376158	0.0
27	2.3419834492029645E-4	0.0	0.0	1.9815521963706282	0.0
28	2.3419834492029645E-4	0.0	0.0	2.0363546090819775	0.0
29	2.3419834492029645E-4	0.0	0.0	2.105911517523306	0.0
30	2.3419834492029645E-4	0.0	0.0	2.194555591075638	0.0
31	2.3419834492029645E-4	0.0	0.0	2.269616160622593	0.0
32	2.3419834492029645E-4	0.0	0.0	2.3409295566508233	0.0
33	2.3419834492029645E-4	0.0	0.0	2.4106035642646115	0.0
34	2.3419834492029645E-4	0.0	0.0	2.486015431328947	0.0
35	2.3419834492029645E-4	0.0	0.0	2.5916388848880003	0.0
36	2.3419834492029645E-4	0.0	0.0	2.6761844874042273	0.0
37	2.3419834492029645E-4	0.0	0.0	2.7579197097814108	0.0
38	2.3419834492029645E-4	0.0	0.0	2.854994923750874	0.0
39	2.3419834492029645E-4	0.0	0.0	3.0048818644998634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.032266E-4	45.000004	1
AACCGTT	20	7.032266E-4	45.000004	33
CGCATCG	20	7.032266E-4	45.000004	21
TACGACA	20	7.032266E-4	45.000004	22
TCGTCGG	20	7.032266E-4	45.000004	1
CATACGA	20	7.032266E-4	45.000004	18
GCGATCG	20	7.032266E-4	45.000004	9
CGTACCC	20	7.032266E-4	45.000004	35
GATTCGC	20	7.032266E-4	45.000004	10
CGACCGT	25	3.8899605E-5	45.0	31
ATCGTAG	25	3.8899605E-5	45.0	1
CGTTTTT	2420	0.0	42.210747	1
ATACGCG	50	1.0804797E-9	40.5	1
ATAGGGC	225	0.0	40.0	4
CGCTGAA	130	0.0	39.807693	37
TCGATCA	85	0.0	39.705883	17
CCGTGCA	40	3.4568257E-7	39.375004	41
AATACGG	40	3.4568257E-7	39.375004	2
TCACGAC	120	0.0	39.374996	25
GTGGCGA	120	0.0	39.374996	28
>>END_MODULE