FastQCFastQC Report
Thu 26 May 2016
SRR1546793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546793_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences800057
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36620.45771738763613096No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11980.14973933107266107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG302.1646701E-645.0000041
ACGTTCA302.1646701E-645.00000418
GACCGTA207.031999E-445.00000416
CGTTGCA207.031999E-445.00000433
ACTCTCG207.031999E-445.00000436
ATATGCG302.1646701E-645.0000041
ATCGTAC207.031999E-445.00000421
ATATACG302.1646701E-645.0000041
GTCGTAC207.031999E-445.00000431
TCGTACT207.031999E-445.00000413
CGGTCTA800.045.00000431
GACGATA207.031999E-445.00000410
CAATCGT207.031999E-445.00000438
TCGACCC302.1646701E-645.00000419
CGTTAGG1150.043.043482
TAGGGTA2950.041.9491545
ACGGGTG700.041.7857175
CGTTTTT23150.041.3066941
TTTAGCG603.6379788E-1241.2500041
CGTATGG1100.040.9090922