##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546793_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	800057
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61602735805074	31.0	31.0	33.0	30.0	34.0
2	31.99972001994858	31.0	31.0	34.0	30.0	34.0
3	32.08333281253711	33.0	31.0	34.0	30.0	34.0
4	35.78796635739703	37.0	35.0	37.0	35.0	37.0
5	35.720612406366044	37.0	35.0	37.0	33.0	37.0
6	35.76947892462662	37.0	35.0	37.0	33.0	37.0
7	36.072382342758075	37.0	35.0	37.0	35.0	37.0
8	35.95388578563777	37.0	35.0	37.0	35.0	37.0
9	37.75091024764485	39.0	38.0	39.0	35.0	39.0
10	37.43990365686445	39.0	37.0	39.0	35.0	39.0
11	37.089283638540756	39.0	37.0	39.0	33.0	39.0
12	36.17014662705282	37.0	35.0	39.0	33.0	39.0
13	35.846008471896376	37.0	35.0	39.0	32.0	39.0
14	36.770260118966526	38.0	35.0	41.0	32.0	41.0
15	37.03469752780114	38.0	35.0	41.0	32.0	41.0
16	37.148375678232924	38.0	35.0	41.0	33.0	41.0
17	37.078875630111355	38.0	35.0	41.0	33.0	41.0
18	36.97735411351941	38.0	35.0	41.0	32.0	41.0
19	36.935930814929435	38.0	35.0	41.0	32.0	41.0
20	36.835621711953024	38.0	35.0	41.0	32.0	41.0
21	36.65593701448772	38.0	35.0	41.0	32.0	41.0
22	36.57650017436258	38.0	35.0	41.0	32.0	41.0
23	36.51269597041211	38.0	35.0	40.0	32.0	41.0
24	36.477384736337534	38.0	35.0	40.0	32.0	41.0
25	36.35394478143432	37.0	35.0	40.0	31.0	41.0
26	36.29206169060454	37.0	35.0	40.0	31.0	41.0
27	36.19478112184507	37.0	35.0	40.0	31.0	41.0
28	36.206125313571405	37.0	35.0	40.0	31.0	41.0
29	36.15303534623158	37.0	35.0	40.0	31.0	41.0
30	36.18011091709716	37.0	35.0	40.0	31.0	41.0
31	36.021878441161064	37.0	35.0	40.0	31.0	41.0
32	35.83600418470184	37.0	35.0	40.0	30.0	41.0
33	35.75856345235402	37.0	35.0	40.0	30.0	41.0
34	35.59404767410322	37.0	35.0	40.0	30.0	41.0
35	35.46211957398035	37.0	35.0	40.0	29.0	41.0
36	35.33470240245383	36.0	35.0	40.0	29.0	41.0
37	35.04446433191635	36.0	34.0	40.0	27.0	41.0
38	35.14971933249756	36.0	34.0	40.0	28.0	41.0
39	35.07172488960162	36.0	34.0	40.0	28.0	41.0
40	34.92849384481356	36.0	34.0	40.0	27.0	41.0
41	34.88984409860797	36.0	34.0	40.0	27.0	41.0
42	34.86909932667297	36.0	34.0	40.0	27.0	41.0
43	34.75299009945542	35.0	34.0	40.0	27.0	41.0
44	34.63256742957064	35.0	34.0	40.0	27.0	41.0
45	34.59961977709088	35.0	34.0	40.0	27.0	41.0
46	34.43575395253088	35.0	34.0	40.0	26.0	41.0
47	34.30331588874293	35.0	34.0	40.0	26.0	41.0
48	34.20636154673979	35.0	34.0	40.0	25.0	41.0
49	34.25700293854063	35.0	34.0	39.0	26.0	41.0
50	34.1227412546856	35.0	34.0	39.0	26.0	41.0
51	33.51760812042142	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	3.0
13	8.0
14	13.0
15	35.0
16	58.0
17	152.0
18	376.0
19	674.0
20	1159.0
21	1812.0
22	2755.0
23	4018.0
24	5907.0
25	8095.0
26	11011.0
27	13066.0
28	14183.0
29	15818.0
30	18544.0
31	22763.0
32	29044.0
33	39462.0
34	77502.0
35	135042.0
36	53658.0
37	68228.0
38	101800.0
39	174822.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.82598239875409	23.982041279558832	30.043484401736375	14.148491919950704
2	30.61181890790281	25.36169297937522	30.490327564161053	13.536160548560916
3	29.791252373268406	24.875102648936263	31.45563378609274	13.87801119170259
4	26.653600930933667	27.408547141016204	30.206722771002564	15.73112915704756
5	24.40951082235391	31.363765331720117	29.731506630152605	14.495217215773376
6	21.636208420150062	39.67742298361242	27.146565807186242	11.539802789051281
7	86.8134395424326	4.617545999847511	6.506661400375223	2.062353057344664
8	88.72667822417652	3.1367765046740423	6.0523187722874745	2.084226498861956
9	85.24605121885067	4.398436611391438	7.290605544354965	3.0649066254029402
10	56.04813157062559	19.550482028155493	13.0678189179021	11.333567483316815
11	47.468117896599864	19.944703939844285	18.58580076169573	14.001377401860118
12	41.1540677726712	20.983879898557227	22.54814344477956	15.313908883992017
13	22.98348742652086	36.44377838079037	23.79692946877535	16.77580472391342
14	16.933293502837923	38.76973765619199	28.33160637304592	15.96536246792416
15	16.444203350511277	22.106049943941493	45.83023459578505	15.61951210976218
16	18.732415315408776	18.21432722918492	43.52964851254348	19.523608942862822
17	20.51666318774787	18.467684177502353	27.054447370624846	33.961205264124935
18	24.714614083746532	22.49152247902337	31.164904500554336	21.62895893667576
19	30.34521290358062	24.132655548292185	25.009718057588397	20.5124134905388
20	32.35269487049048	23.364335291110507	23.898422237415584	20.38454760098343
21	25.817160527312428	25.446686923556694	27.91813583282191	20.81801671630896
22	25.263449979188984	22.298786211482433	27.289805601350903	25.147958207977684
23	23.018484932948528	27.245308771750015	25.339194582386003	24.397011712915454
24	23.66981352578629	23.165099486661575	34.23868549365858	18.92640149389356
25	20.35367480067045	24.52037792307298	32.8282859846236	22.297661291632974
26	20.047946583805903	31.590624168028025	26.335498595725053	22.025930652441016
27	20.13956505598976	30.318589800476715	29.881120970130876	19.660724173402645
28	17.971719514984557	26.484237998042637	36.16042356982065	19.383618917152155
29	18.595550067057722	24.84485480409521	36.491274996656486	20.068320132190582
30	21.55683907521589	26.45861482369381	31.80560885036941	20.178937250720885
31	30.265718567552064	24.353014847692105	24.982345007918187	20.39892157683765
32	30.57444657068184	25.62242440226134	25.500808067425197	18.302320959631626
33	28.323731934099694	26.04989394505641	24.921349353858538	20.705024766985353
34	21.384601347154014	27.39392318297321	28.046876660037974	23.174598809834798
35	21.902564442283488	26.111889527871142	29.7452556505349	22.240290379310473
36	30.095105723717186	23.7795557066559	26.51873554009277	19.606603029534146
37	22.844122356282114	29.197419683847524	28.13262055078576	19.825837409084603
38	23.77655592039067	30.029485399165313	23.710310640366874	22.483648040077146
39	22.67213461040901	29.21104371313544	25.911153830289592	22.205667846165962
40	24.274645431513004	25.103336387282404	25.583552171907755	25.03846600929684
41	20.293804066460265	23.01248536041807	27.35180118416563	29.34190938895604
42	24.648868768100275	25.11558551453209	24.55687532263326	25.678670394734375
43	24.283394808119922	25.52793113490664	25.240951582199767	24.947722474773673
44	21.48471921375602	29.35040878337418	26.97757784757836	22.187294155291433
45	20.306053193709946	33.97695414201738	22.88811922150547	22.828873442767204
46	23.690937020737273	29.69638413263055	26.1695104223824	20.443168424249773
47	23.7165601950861	26.610728985559778	26.924706614653704	22.748004204700415
48	26.209882545868606	22.96911345066664	28.51721814820694	22.303785855257814
49	22.91361740475991	22.472898805960074	30.583445929477527	24.03003785980249
50	21.36922744254472	29.95436575144021	26.34087321278359	22.33553359323148
51	20.96013159062417	31.85748015453899	24.25352193656202	22.928866318274824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	557.0
1	994.0
2	1431.0
3	3887.5
4	6344.0
5	4552.0
6	2760.0
7	2851.0
8	2942.0
9	3129.0
10	3316.0
11	3315.0
12	3314.0
13	3255.0
14	3196.0
15	2944.0
16	2692.0
17	2500.5
18	2309.0
19	2362.0
20	2415.0
21	2559.0
22	2703.0
23	2704.5
24	2706.0
25	2990.5
26	4233.5
27	5192.0
28	5634.5
29	6077.0
30	7837.0
31	9597.0
32	10111.5
33	10626.0
34	12569.0
35	14512.0
36	16113.5
37	17715.0
38	22339.0
39	26963.0
40	37887.5
41	48812.0
42	60509.5
43	72207.0
44	71612.0
45	71017.0
46	67661.0
47	64305.0
48	61494.5
49	58684.0
50	58210.5
51	57737.0
52	54606.0
53	51475.0
54	47940.5
55	44406.0
56	42329.0
57	40252.0
58	38895.0
59	37538.0
60	35204.5
61	32871.0
62	29534.0
63	26197.0
64	23310.0
65	20423.0
66	17641.0
67	14859.0
68	12789.5
69	10720.0
70	8731.5
71	6743.0
72	5696.0
73	4649.0
74	3881.5
75	2410.5
76	1707.0
77	1237.0
78	767.0
79	626.0
80	485.0
81	345.0
82	205.0
83	151.5
84	98.0
85	75.0
86	52.0
87	46.0
88	40.0
89	34.0
90	28.0
91	16.5
92	5.0
93	8.5
94	12.0
95	9.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	800057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.992097781291527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98215781614543	16.58001179853924
2	5.213366544393688	2.1887900053925247
3	1.7459019494525447	1.0995043331836596
4	1.0647555122108254	0.8940580730199515
5	0.7360818166208638	0.7725950734767937
6	0.6247263838946302	0.7868590402361249
7	0.4812057803968298	0.7071063156509064
8	0.44834435053988425	0.7529350756978316
9	0.404451586758116	0.7641258531322397
>10	8.306909250049817	46.06112334678501
>50	1.8802303864792247	25.682361327540825
>100	0.11067216719651816	3.1002080793168045
>500	0.0	0.0
>1k	0.00119645586158398	0.6103216780280986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3662	0.45771738763613096	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1198	0.14973933107266107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009374332078839382	0.0
2	0.0	0.0	0.0	0.04412185631773736	0.0
3	0.0	0.0	0.0	0.06649526221256735	0.0
4	0.0	0.0	0.0	0.10749234117069159	0.0
5	0.0	0.0	0.0	0.20398546603554496	0.0
6	0.0	0.0	0.0	0.3282266138537629	0.0
7	0.0	0.0	0.0	0.400846439691172	0.0
8	0.0	0.0	0.0	0.5843333662476549	0.0
9	0.0	0.0	0.0	0.6730770432606676	0.0
10	0.0	0.0	0.0	0.8271910626367871	0.0
11	0.0	0.0	0.0	0.993304227073821	0.0
12	0.0	0.0	0.0	1.134919137011488	0.0
13	0.0	0.0	0.0	1.1942899068441373	0.0
14	0.0	0.0	0.0	1.2164133305501983	0.0
15	0.0	0.0	0.0	1.2442863445979473	0.0
16	0.0	0.0	0.0	1.3092817136779005	0.0
17	0.0	0.0	0.0	1.3804016463826951	0.0
18	0.0	0.0	0.0	1.4847692101937737	0.0
19	0.0	0.0	0.0	1.541515167044348	0.0
20	0.0	0.0	0.0	1.6051356340860714	0.0
21	0.0	0.0	0.0	1.6817551749437853	0.0
22	0.0	0.0	0.0	1.757624769235192	0.0
23	0.0	0.0	0.0	1.8441186065492834	0.0
24	0.0	0.0	0.0	1.9067391448359305	0.0
25	0.0	0.0	0.0	1.9607352976100454	0.0
26	0.0	0.0	0.0	2.016606316799928	0.0
27	0.0	0.0	0.0	2.076352059915731	0.0
28	0.0	0.0	0.0	2.133972954426997	0.0
29	0.0	0.0	0.0	2.2017181275834097	0.0
30	0.0	0.0	0.0	2.2804625170456605	0.0
31	0.0	0.0	0.0	2.353457316166223	0.0
32	0.0	0.0	0.0	2.42407728449348	0.0
33	0.0	0.0	0.0	2.498446985652272	0.0
34	0.0	0.0	0.0	2.5746915532268324	0.0
35	0.0	0.0	0.0	2.682683858775062	0.0
36	0.0	0.0	0.0	2.7648030077856953	0.0
37	0.0	0.0	0.0	2.8475471122682507	0.0
38	0.0	0.0	0.0	2.944290219321873	0.0
39	0.0	0.0	0.0	3.0905297997517676	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAG	30	2.1646701E-6	45.000004	1
ACGTTCA	30	2.1646701E-6	45.000004	18
GACCGTA	20	7.031999E-4	45.000004	16
CGTTGCA	20	7.031999E-4	45.000004	33
ACTCTCG	20	7.031999E-4	45.000004	36
ATATGCG	30	2.1646701E-6	45.000004	1
ATCGTAC	20	7.031999E-4	45.000004	21
ATATACG	30	2.1646701E-6	45.000004	1
GTCGTAC	20	7.031999E-4	45.000004	31
TCGTACT	20	7.031999E-4	45.000004	13
CGGTCTA	80	0.0	45.000004	31
GACGATA	20	7.031999E-4	45.000004	10
CAATCGT	20	7.031999E-4	45.000004	38
TCGACCC	30	2.1646701E-6	45.000004	19
CGTTAGG	115	0.0	43.04348	2
TAGGGTA	295	0.0	41.949154	5
ACGGGTG	70	0.0	41.785717	5
CGTTTTT	2315	0.0	41.306694	1
TTTAGCG	60	3.6379788E-12	41.250004	1
CGTATGG	110	0.0	40.909092	2
>>END_MODULE