##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546792_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1445329
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67912080917217	31.0	31.0	33.0	30.0	34.0
2	32.04655133883012	33.0	31.0	34.0	30.0	34.0
3	32.092306319184075	33.0	31.0	34.0	30.0	34.0
4	35.789683179400676	37.0	35.0	37.0	35.0	37.0
5	35.72343943835625	37.0	35.0	37.0	33.0	37.0
6	35.78549451370588	37.0	35.0	37.0	35.0	37.0
7	36.10677707290174	37.0	35.0	37.0	35.0	37.0
8	35.99918980384397	37.0	35.0	37.0	35.0	37.0
9	37.79969404889821	39.0	38.0	39.0	35.0	39.0
10	37.5168871585639	39.0	37.0	39.0	35.0	39.0
11	37.1251023123455	39.0	37.0	39.0	34.0	39.0
12	35.90123079243549	37.0	35.0	39.0	33.0	39.0
13	35.47467946744305	37.0	35.0	39.0	31.0	39.0
14	36.322660100226315	38.0	35.0	41.0	31.0	41.0
15	36.645370707984135	38.0	35.0	41.0	32.0	41.0
16	36.826722497092355	38.0	35.0	41.0	33.0	41.0
17	36.71798185741793	37.0	35.0	41.0	32.0	41.0
18	36.67103060963974	37.0	35.0	40.0	32.0	41.0
19	36.59937702765253	37.0	35.0	40.0	32.0	41.0
20	36.448585062639715	36.0	35.0	40.0	32.0	41.0
21	36.27605894574869	36.0	35.0	40.0	32.0	41.0
22	36.192274561708786	36.0	35.0	40.0	31.0	41.0
23	36.13653707910102	35.0	35.0	40.0	31.0	41.0
24	36.10713616069421	35.0	35.0	40.0	31.0	41.0
25	36.018373671323275	35.0	35.0	40.0	31.0	41.0
26	35.938469372717215	35.0	35.0	40.0	31.0	41.0
27	35.84163674844966	35.0	35.0	40.0	31.0	41.0
28	35.88515832727358	36.0	35.0	40.0	31.0	41.0
29	35.85931991954773	36.0	35.0	40.0	31.0	41.0
30	35.88692401522422	36.0	35.0	40.0	31.0	41.0
31	35.720122546492874	36.0	35.0	40.0	31.0	41.0
32	35.47278647283767	35.0	34.0	40.0	30.0	41.0
33	35.37173266432764	35.0	34.0	40.0	30.0	41.0
34	35.1945508600464	35.0	34.0	40.0	29.0	41.0
35	35.1044218997889	35.0	34.0	40.0	29.0	41.0
36	34.95052129999467	35.0	34.0	40.0	29.0	41.0
37	34.660246213837816	35.0	34.0	40.0	27.0	41.0
38	34.7642944962704	35.0	34.0	40.0	28.0	41.0
39	34.725675607422254	35.0	34.0	40.0	27.0	41.0
40	34.5806117499891	35.0	34.0	40.0	27.0	41.0
41	34.586592395226276	35.0	34.0	40.0	27.0	41.0
42	34.59704607047946	35.0	34.0	40.0	27.0	41.0
43	34.45274397732281	35.0	34.0	40.0	27.0	41.0
44	34.33573324827773	35.0	34.0	40.0	27.0	41.0
45	34.28922065495122	35.0	34.0	40.0	27.0	41.0
46	34.151258986708214	35.0	34.0	39.0	26.0	41.0
47	34.04194269955145	35.0	33.0	39.0	26.0	41.0
48	33.97214129101402	35.0	34.0	39.0	25.0	41.0
49	34.05165605893191	35.0	34.0	39.0	26.0	41.0
50	33.89248191934155	35.0	34.0	39.0	26.0	41.0
51	33.294544702278856	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	3.0
13	10.0
14	27.0
15	55.0
16	143.0
17	267.0
18	614.0
19	1205.0
20	2092.0
21	3567.0
22	5343.0
23	7659.0
24	11060.0
25	15530.0
26	20752.0
27	24408.0
28	26437.0
29	29118.0
30	34464.0
31	42740.0
32	54892.0
33	75576.0
34	156246.0
35	304524.0
36	84572.0
37	102868.0
38	157922.0
39	283143.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.61561900439277	23.716814649121414	30.147737989066847	13.519828357418968
2	30.774792452099142	26.063546777238955	29.84905166920473	13.312609101457177
3	29.124026432736077	25.080933130103944	32.03900288446437	13.756037552695616
4	25.95713501908562	27.526604669248318	31.071749061978277	15.444511249687787
5	23.413700271702844	32.14797461339252	29.57036079674593	14.867964318158702
6	22.079401990827	38.41928031610796	27.737006591578805	11.764311101486236
7	86.40274982374255	5.043834310388846	6.679032939905031	1.8743829259635696
8	88.52593423365892	4.007945595777848	5.731290246027029	1.7348299245362127
9	85.42982255251226	4.491157376624976	6.941187784926477	3.1378322859362817
10	61.7746547671845	17.734508890363372	11.090900410909905	9.399935931542231
11	56.139190454214926	17.198022042040257	15.656435316803302	11.00635218694152
12	48.68331016675096	20.181840951091413	19.20573101349243	11.929117868665196
13	23.02257825034992	44.55546107495248	19.628195379737072	12.793765294960524
14	14.127094938245893	46.177167966601374	25.827614335559584	13.868122759593144
15	13.26867446788932	21.5436762148964	52.23606528340606	12.951584033808219
16	15.305442567055666	15.738700323594143	50.97510670580885	17.98075040354134
17	16.426017882433687	15.890499671701045	27.776720732788174	39.906761713077096
18	23.166282555736444	22.178479778652473	32.601366194132964	22.05387147147812
19	31.76605464914909	23.80973466940745	23.632543178750307	20.79166750269316
20	35.823262385242394	22.142294245808394	22.12202204480779	19.912421324141423
21	24.285404914728755	28.20513530137429	26.309165594823046	21.200294189073908
22	23.746634849228098	24.17304295423395	24.762182174439175	27.318140022098774
23	21.761273730756113	31.028298747205653	23.00452007812754	24.205907443910693
24	22.407493380399895	22.443886478441932	37.75707814622138	17.391541994936794
25	17.94691727627412	23.574701676919236	36.15571264397241	22.322668402834232
26	16.608121749442514	35.63257915671795	27.254071564328953	20.50522752951058
27	18.905038230050046	33.47251732996432	28.78029846491699	18.842145975068654
28	14.454217690228315	28.470403624365108	40.661814714850394	16.413563970556186
29	15.911740510292121	24.389533455704548	39.398918862072236	20.299807171931096
30	18.90510741844936	29.10430773893003	32.73040255886376	19.260182283756848
31	31.507151658895655	25.17246938240359	23.172232758077918	20.148146200622836
32	32.8336316506484	26.67987703837673	24.508329937336068	15.978161373638805
33	29.851542451580226	27.84763884209062	23.84419049226854	18.456628214060604
34	20.13555391194669	28.820842866918188	28.178221014039018	22.8653822070961
35	20.712170031875097	26.109211120789798	31.02892144279953	22.149697404535576
36	31.94712069016812	24.04283038671472	25.80914103294129	18.20090789017587
37	22.396354048109462	31.054659527346367	28.49870167968677	18.0502847448574
38	22.288627710369056	32.01935337905764	22.581294639490384	23.110724271082915
39	21.31058049758913	31.50839705008341	26.08333465944432	21.097687792883143
40	25.546916999520526	25.335546439599565	24.22846286208884	24.88907369879107
41	17.604019569246866	23.6719805663624	26.963341910388568	31.76065795400217
42	24.422121191784015	25.871618157526765	22.532170875973566	27.174089774715654
43	24.461143449000193	26.967354837549095	23.033579205841715	25.537922507608997
44	21.360534521897783	32.00842161196517	26.202200329475154	20.428843536661894
45	18.90303176646978	37.36713232765689	20.716113770636305	23.013722135237032
46	23.083602418549688	33.18891408115384	24.477471911239586	19.250011589056886
47	23.556989446693454	27.288596575589363	25.99062220435624	23.163791773360945
48	26.108034918001366	21.527416941056327	29.773359560349235	22.59118858059307
49	22.36618790600618	21.57840879135477	31.542022612152664	24.51338069048639
50	19.773006699512706	33.001136765400815	25.053188581976837	22.172667953109638
51	18.483819254993154	35.614797738092854	21.792339322050548	24.109043684863448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1077.0
1	1666.5
2	2256.0
3	6690.0
4	11124.0
5	8072.0
6	5020.0
7	5288.5
8	5557.0
9	5951.5
10	6346.0
11	6345.5
12	6345.0
13	6061.0
14	5777.0
15	5580.0
16	5383.0
17	5042.5
18	4702.0
19	4436.5
20	4171.0
21	4124.0
22	4077.0
23	4185.0
24	4293.0
25	4607.5
26	7052.5
27	9183.0
28	9780.0
29	10377.0
30	12505.5
31	14634.0
32	17303.0
33	19972.0
34	22262.5
35	24553.0
36	25699.5
37	26846.0
38	33887.5
39	40929.0
40	69228.0
41	97527.0
42	124421.5
43	151316.0
44	151739.0
45	152162.0
46	142696.5
47	133231.0
48	124413.5
49	115596.0
50	109410.5
51	103225.0
52	99532.0
53	95839.0
54	88744.5
55	81650.0
56	76334.5
57	71019.0
58	66503.5
59	61988.0
60	58298.0
61	54608.0
62	47565.5
63	40523.0
64	32015.0
65	23507.0
66	20027.5
67	16548.0
68	13377.0
69	10206.0
70	8930.5
71	7655.0
72	6205.5
73	4756.0
74	3833.5
75	2373.0
76	1835.0
77	1385.5
78	936.0
79	672.5
80	409.0
81	289.5
82	170.0
83	115.0
84	60.0
85	49.0
86	38.0
87	29.0
88	20.0
89	16.0
90	12.0
91	12.5
92	13.0
93	17.5
94	22.0
95	12.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1445329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.597504544647002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17901436587779	14.637430833227928
2	6.504481522165917	2.2892528629377438
3	1.7332203900592653	0.9150106107282832
4	0.8750485577576314	0.6159468388770689
5	0.49867597121073515	0.43877263348435847
6	0.37638317511431135	0.39740427807616574
7	0.27290742692427983	0.3361742779897554
8	0.20875433024757478	0.2938844220197151
9	0.1815268428427385	0.2874977497710462
>10	2.7337460822401756	12.538518614706884
>50	1.7440060972203373	22.861488161012794
>100	1.6894688565858063	43.360119497855834
>500	0.001975986966767025	0.2444502505837572
>1k	0.0	0.0
>5k	7.9039478670681E-4	0.7840489687286709
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6040	0.41789793188955593	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5234	0.36213208203806885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0756519795838872E-4	0.0	0.0	0.022071099382908667	0.0
2	2.0756519795838872E-4	0.0	0.0	0.07271700768475552	0.0
3	2.0756519795838872E-4	0.0	0.0	0.10011561381526282	0.0
4	2.0756519795838872E-4	0.0	0.0	0.1561582172640278	0.0
5	2.0756519795838872E-4	0.0	0.0	0.28215029242476974	0.0
6	2.0756519795838872E-4	0.0	0.0	0.40219216524403784	0.0
7	2.0756519795838872E-4	0.0	0.0	0.49359004074504836	0.0
8	2.0756519795838872E-4	0.0	0.0	0.7187982805299001	0.0
9	2.0756519795838872E-4	0.0	0.0	0.8288770238471656	0.0
10	2.0756519795838872E-4	0.0	0.0	1.0796157829808992	0.0
11	2.0756519795838872E-4	0.0	0.0	1.2882188069290799	0.0
12	2.0756519795838872E-4	0.0	0.0	1.5054703807921934	0.0
13	2.0756519795838872E-4	0.0	0.0	1.5827538228320335	0.0
14	2.0756519795838872E-4	0.0	0.0	1.6083535305802348	0.0
15	2.0756519795838872E-4	0.0	0.0	1.6518038453528574	0.0
16	2.0756519795838872E-4	0.0	0.0	1.7355909969287269	0.0
17	2.0756519795838872E-4	0.0	0.0	1.8265045536345013	0.0
18	2.0756519795838872E-4	0.0	0.0	1.966195931860497	0.0
19	2.0756519795838872E-4	0.0	0.0	2.0350383891833626	0.0
20	2.0756519795838872E-4	0.0	0.0	2.0998679193456993	0.0
21	2.0756519795838872E-4	0.0	0.0	2.1870453024882224	0.0
22	2.0756519795838872E-4	0.0	0.0	2.2751221348218986	0.0
23	2.0756519795838872E-4	0.0	0.0	2.3783512266065374	0.0
24	2.0756519795838872E-4	0.0	0.0	2.4504455386974175	0.0
25	2.0756519795838872E-4	0.0	0.0	2.5046892437638766	0.0
26	2.0756519795838872E-4	0.0	0.0	2.562323180396989	0.0
27	2.0756519795838872E-4	0.0	0.0	2.6230014066001583	0.0
28	2.0756519795838872E-4	0.0	0.0	2.6831261256087715	0.0
29	2.0756519795838872E-4	0.0	0.0	2.74643351098608	0.0
30	2.0756519795838872E-4	0.0	0.0	2.8394919080707575	0.0
31	2.0756519795838872E-4	0.0	0.0	2.9287449431928647	0.0
32	2.0756519795838872E-4	0.0	0.0	3.001116008881023	0.0
33	2.0756519795838872E-4	0.0	0.0	3.0753551613508066	0.0
34	2.0756519795838872E-4	0.0	0.0	3.155060197366828	0.0
35	2.0756519795838872E-4	0.0	0.0	3.2657616362779684	0.0
36	2.0756519795838872E-4	0.0	0.0	3.3512093094375053	0.0
37	2.0756519795838872E-4	0.0	0.0	3.4390093881739037	0.0
38	2.0756519795838872E-4	0.0	0.0	3.522450597753176	0.0
39	2.0756519795838872E-4	0.0	0.0	3.6157857484351315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	30	2.1658907E-6	45.000004	13
CGTTATG	30	2.1658907E-6	45.000004	17
CCATACT	35	1.2122109E-7	45.000004	27
TATAGTC	35	1.2122109E-7	45.000004	43
ACGCGAG	25	3.891306E-5	45.0	1
AACGTTG	25	3.891306E-5	45.0	44
AACGTCG	40	6.8175723E-9	45.0	23
ATCTCGA	25	3.891306E-5	45.0	11
TCGTTAA	20	7.03389E-4	45.0	17
CTCGTTA	20	7.03389E-4	45.0	16
GTCGATC	20	7.03389E-4	45.0	14
ACGTTGT	20	7.03389E-4	45.0	15
CGCATTA	20	7.03389E-4	45.0	15
TAAACGG	100	0.0	45.0	2
CACGATT	25	3.891306E-5	45.0	11
CGCGAGT	20	7.03389E-4	45.0	17
TATGCGA	20	7.03389E-4	45.0	37
CCGCTAT	20	7.03389E-4	45.0	40
ATCGTTC	20	7.03389E-4	45.0	2
GTAATCG	25	3.891306E-5	45.0	32
>>END_MODULE