FastQCFastQC Report
Thu 26 May 2016
SRR1546791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546791_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1670557
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77190.46206145614905686No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51310.3071430666538167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG1450.045.0000041
CGGCATA351.2123746E-745.0000042
GTAGGCC700.045.0000041
ATATCTC351.2123746E-745.00000413
TATTCCG351.2123746E-745.00000440
GGAACGT700.045.0000048
GTGCGTC351.2123746E-745.00000441
TGTCGAT351.2123746E-745.00000411
CGATAAT700.045.00000410
CCTTCGT207.034206E-445.023
CAGGTCG253.8915678E-545.045
CGGCGTA207.034206E-445.019
AAGTACG502.1827873E-1145.01
AGCCGAA406.8175723E-945.037
GCCCATA406.8175723E-945.012
GCCTTCG207.034206E-445.022
CGTATTC406.8175723E-945.022
CGTATAT207.034206E-445.023
GAATGTA800.045.028
AGTCAAC253.8915678E-545.023