##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546791_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1670557
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652521883419723	31.0	31.0	33.0	30.0	34.0
2	32.01272389987292	33.0	31.0	34.0	30.0	34.0
3	32.06853941529681	33.0	31.0	34.0	30.0	34.0
4	35.747223830135695	37.0	35.0	37.0	35.0	37.0
5	35.663185991259205	37.0	35.0	37.0	33.0	37.0
6	35.73077482540255	37.0	35.0	37.0	33.0	37.0
7	36.09044767703227	37.0	35.0	37.0	35.0	37.0
8	35.99953548427261	37.0	35.0	37.0	35.0	37.0
9	37.85831432270793	39.0	38.0	39.0	35.0	39.0
10	37.502358794102804	39.0	37.0	39.0	35.0	39.0
11	37.06465568071009	39.0	37.0	39.0	33.0	39.0
12	35.443178532669045	35.0	35.0	39.0	32.0	39.0
13	34.839662459886135	35.0	35.0	39.0	30.0	39.0
14	35.5582305781844	35.0	35.0	40.0	31.0	41.0
15	36.00959919356239	35.0	35.0	40.0	31.0	41.0
16	36.27930983498318	35.0	35.0	40.0	32.0	41.0
17	36.23801881647858	35.0	35.0	40.0	32.0	41.0
18	36.192666278373025	36.0	35.0	40.0	32.0	41.0
19	36.14463918321853	36.0	35.0	40.0	32.0	41.0
20	35.977438064070846	35.0	35.0	40.0	32.0	41.0
21	35.7398209100318	35.0	35.0	40.0	31.0	41.0
22	35.65443681358972	35.0	35.0	40.0	31.0	41.0
23	35.672463136546675	35.0	35.0	40.0	31.0	41.0
24	35.6061481290372	35.0	35.0	40.0	31.0	41.0
25	35.48501368106566	35.0	35.0	40.0	31.0	41.0
26	35.40787354157924	35.0	34.0	40.0	31.0	41.0
27	35.286769023744775	35.0	34.0	40.0	31.0	41.0
28	35.406825986781655	35.0	35.0	40.0	31.0	41.0
29	35.43999276887888	35.0	35.0	40.0	31.0	41.0
30	35.51423567109653	36.0	35.0	40.0	31.0	41.0
31	35.264543502556336	35.0	34.0	40.0	31.0	41.0
32	34.99770196407545	35.0	34.0	40.0	30.0	41.0
33	34.92428752805202	35.0	34.0	40.0	30.0	41.0
34	34.81374954581017	35.0	34.0	40.0	29.0	41.0
35	34.75524211385783	35.0	34.0	40.0	29.0	41.0
36	34.55307122115558	35.0	34.0	39.0	28.0	41.0
37	34.25253014413755	35.0	34.0	39.0	27.0	41.0
38	34.40339300005926	35.0	34.0	39.0	28.0	41.0
39	34.40236280474117	35.0	34.0	39.0	27.0	41.0
40	34.24836985508426	35.0	34.0	39.0	27.0	41.0
41	34.26785557152495	35.0	34.0	39.0	27.0	41.0
42	34.274403686914006	35.0	34.0	39.0	27.0	41.0
43	34.14324384022814	35.0	34.0	39.0	27.0	41.0
44	34.04675566293159	35.0	34.0	39.0	27.0	41.0
45	34.00870847268306	35.0	34.0	39.0	27.0	41.0
46	33.887839804328735	35.0	34.0	38.0	27.0	41.0
47	33.80082271960789	35.0	33.0	38.0	26.0	41.0
48	33.73961918090792	35.0	34.0	38.0	26.0	41.0
49	33.88250804971037	35.0	34.0	38.0	27.0	41.0
50	33.696084599328245	35.0	34.0	38.0	26.0	40.0
51	33.05962622047616	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	6.0
13	5.0
14	15.0
15	46.0
16	115.0
17	305.0
18	695.0
19	1472.0
20	2604.0
21	4195.0
22	6327.0
23	9427.0
24	13502.0
25	19311.0
26	25140.0
27	29844.0
28	32446.0
29	35994.0
30	42677.0
31	52773.0
32	68419.0
33	94877.0
34	204482.0
35	426401.0
36	83367.0
37	93603.0
38	145073.0
39	277364.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.55621568135658	23.71346802294085	31.695296838120456	14.035019457582113
2	28.29822628021672	26.568743239530292	32.17633400117445	12.956696479078536
3	28.245788680062994	25.12281831748333	33.57461014499954	13.056782857454131
4	26.103868350496274	27.329447603404134	31.19336843938878	15.373315606710817
5	22.474899090542856	32.4451664923735	31.32260677127449	13.757327645809154
6	21.02693891917486	39.48910453220093	27.654069870109193	11.82988667851501
7	87.53493595249967	5.287218574403627	5.714261770176055	1.4635837029206427
8	90.5356117749948	2.888976550934808	5.133916412310385	1.4414952617599999
9	89.1193775489253	3.386834451024419	5.8411655513699925	1.6526224486802905
10	69.24684401669623	13.676097253790203	9.029204031948627	8.047854697564944
11	64.97102463429863	14.755258276131853	12.446746803611012	7.826970285958515
12	54.946763265186405	19.420468741862745	16.59254967055898	9.040218322391873
13	19.908808858362807	53.89334216072843	17.405811355134844	8.792037625773919
14	11.336338718164061	54.34001952642142	25.05469732550281	9.2689444299117
15	9.123543824006006	20.293710421134985	61.05622256528811	9.526523189570904
16	10.891696601792097	12.965974821571487	58.179337789731214	17.962990786905205
17	12.482782688648157	13.541591217779459	27.322264370506367	46.65336172306602
18	22.004217754916475	20.491907788839292	36.63275183067684	20.871122625567402
19	32.7919969207875	23.01172602910287	22.865427519084953	21.330849531024683
20	36.110830100379694	21.88497608881349	21.788601047435076	20.21559276337174
21	21.17018455521123	28.385801861295363	27.687831064728712	22.7561825187647
22	22.703505477514383	23.794279393040764	24.296806394513926	29.205408734930927
23	18.1240747846377	32.879213340221256	21.947290634201646	27.0494212409394
24	19.993271705185755	21.657507047050775	43.03139611518793	15.31782513257554
25	13.918531364089942	23.660910702238837	40.97112519955919	21.449432734112037
26	13.058997687597609	39.826297456477086	26.907432670660146	20.207272185265154
27	15.848785764269044	37.37950875067418	30.603984180126748	16.167721304930033
28	10.992680884279913	28.87294477231247	46.17424008878476	13.960134254622858
29	10.785265034356804	23.34718300542873	45.24778262579487	20.619769334419598
30	16.494079519585384	31.487402106004165	33.61298058072846	18.405537793681987
31	33.983814979075845	25.652222582048985	21.99619647818063	18.367765960694545
32	34.54123385194279	27.36889552406772	24.333800043937444	13.756070580052043
33	29.730323478935468	29.02241587686023	23.260804629833043	17.986456014371257
34	17.952994121122476	29.340633094231443	28.301339014472422	24.405033770173663
35	18.474077807581544	26.3512708635503	32.42906407862767	22.74558725024049
36	34.823175743180265	21.337374300906824	26.906355185725477	16.93309477018743
37	19.418852514460745	31.957784140259804	30.616016095230513	18.007347250048937
38	18.867299948460303	35.52030849590885	21.34964565710718	24.262745898523665
39	18.961998902162573	32.85963903057483	27.693577651046926	20.48478441621567
40	24.291538690388894	25.820250371582652	22.779109003763416	27.10910193426504
41	15.505367371481487	22.228514202149345	27.52267656835415	34.743441858015025
42	24.127581399497295	24.403417542771663	22.218876698011503	29.250124359719543
43	24.384322115318426	26.489488236558227	23.13479875275133	25.991390895372025
44	19.364020503341102	33.810878647062026	27.06438630947642	19.76071454012045
45	15.640651591056157	43.844897240860384	18.85371166622869	21.66073950185477
46	23.23500485167522	35.78237677612916	24.099447070647695	16.88317130154793
47	23.166524698049813	27.659756596153258	24.844587763243037	24.329130942553892
48	25.73195646721423	20.772233452674765	31.190016264036487	22.30579381607452
49	20.982163434112096	20.469160884663022	33.78262459766413	24.766051083560754
50	18.781999057799283	36.30711193931126	24.385160159156495	20.525728843732956
51	17.134883754340617	39.052124530919926	20.36332791996921	23.44966379477025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1137.0
1	1424.0
2	1711.0
3	5159.0
4	8607.0
5	6536.5
6	4466.0
7	5121.0
8	5776.0
9	6442.0
10	7108.0
11	7431.5
12	7755.0
13	7427.5
14	7100.0
15	6545.0
16	5990.0
17	5515.5
18	5041.0
19	5157.5
20	5274.0
21	4794.0
22	4314.0
23	4637.0
24	4960.0
25	4648.5
26	5638.0
27	6939.0
28	8046.5
29	9154.0
30	12304.5
31	15455.0
32	18118.5
33	20782.0
34	20746.0
35	20710.0
36	25518.0
37	30326.0
38	43178.0
39	56030.0
40	98944.5
41	141859.0
42	188350.0
43	234841.0
44	231164.5
45	227488.0
46	201229.0
47	174970.0
48	157600.0
49	140230.0
50	137175.5
51	134121.0
52	118806.5
53	103492.0
54	91336.0
55	79180.0
56	68843.5
57	58507.0
58	52429.0
59	46351.0
60	39866.0
61	33381.0
62	28716.5
63	24052.0
64	19232.5
65	14413.0
66	12160.0
67	9907.0
68	7893.5
69	5880.0
70	4792.5
71	3705.0
72	3407.5
73	3110.0
74	2111.0
75	827.5
76	543.0
77	397.0
78	251.0
79	184.0
80	117.0
81	68.0
82	19.0
83	12.0
84	5.0
85	23.5
86	42.0
87	21.0
88	0.0
89	1.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1670557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.735406026101279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.15835633345725	13.085305013701607
2	7.7010453625818185	2.423581512112985
3	2.138173104866517	1.0093506587749281
4	0.9078168151139172	0.5713946473255841
5	0.540867381487232	0.42553839269879046
6	0.3479449961362348	0.3285033473372337
7	0.2363050740874222	0.26028494007554726
8	0.19288146945273424	0.24280545893998595
9	0.14939253440917952	0.21156769675770715
>10	1.5325142095583286	5.741448439070469
>50	0.8804258819725994	10.401843515619317
>100	2.198958422232171	63.10507974430005
>500	0.01455249391236895	1.4189508977107996
>1k	0.0	0.0
>5k	7.659207322299447E-4	0.7743457355749681
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7719	0.46206145614905686	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5131	0.3071430666538167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023884249385085335	0.0
2	0.0	0.0	0.0	0.08607907422494414	0.0
3	0.0	0.0	0.0	0.12235440035868277	0.0
4	0.0	0.0	0.0	0.2153174061106565	0.0
5	0.0	0.0	0.0	0.3614961955802765	0.0
6	0.0	0.0	0.0	0.5493377358569627	0.0
7	0.0	0.0	0.0	0.6760020759543075	0.0
8	0.0	0.0	0.0	1.0222339016268227	0.0
9	0.0	0.0	0.0	1.15506384996142	0.0
10	0.0	0.0	0.0	1.3712192999101498	0.0
11	0.0	0.0	0.0	1.5566065689467645	0.0
12	0.0	0.0	0.0	1.7242751968355465	0.0
13	0.0	0.0	0.0	1.7932342326541386	0.0
14	0.0	0.0	0.0	1.8101746902380464	0.0
15	0.0	0.0	0.0	1.8434570026643808	0.0
16	0.0	0.0	0.0	1.9204373152188163	0.0
17	0.0	0.0	0.0	2.011245351101459	0.0
18	0.0	0.0	0.0	2.175082921444764	0.0
19	0.0	0.0	0.0	2.2336861298357373	0.0
20	0.0	0.0	0.0	2.292768220419896	0.0
21	0.0	0.0	0.0	2.3929743193437876	0.0
22	0.0	0.0	0.0	2.4811485031639147	0.0
23	0.0	0.0	0.0	2.595840788431643	0.0
24	0.0	0.0	0.0	2.6706062708425993	0.0
25	0.0	0.0	0.0	2.7287904573145365	0.0
26	0.0	0.0	0.0	2.7964325671018706	0.0
27	0.0	0.0	0.0	2.8517434604147	0.0
28	0.0	0.0	0.0	2.9094487646934524	0.0
29	0.0	0.0	0.0	2.976312690916862	0.0
30	0.0	0.0	0.0	3.080948450127712	0.0
31	5.986027414808354E-5	0.0	0.0	3.1869011353698196	0.0
32	5.986027414808354E-5	0.0	0.0	3.265377954777957	0.0
33	5.986027414808354E-5	0.0	0.0	3.3437350536377988	0.0
34	5.986027414808354E-5	0.0	0.0	3.430592311426668	0.0
35	5.986027414808354E-5	0.0	0.0	3.5613870104402303	0.0
36	5.986027414808354E-5	0.0	0.0	3.6538112737248714	0.0
37	5.986027414808354E-5	0.0	0.0	3.7434819643987005	0.0
38	5.986027414808354E-5	0.0	0.0	3.8408147701634845	0.0
39	5.986027414808354E-5	0.0	0.0	3.9370102307194546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	145	0.0	45.000004	1
CGGCATA	35	1.2123746E-7	45.000004	2
GTAGGCC	70	0.0	45.000004	1
ATATCTC	35	1.2123746E-7	45.000004	13
TATTCCG	35	1.2123746E-7	45.000004	40
GGAACGT	70	0.0	45.000004	8
GTGCGTC	35	1.2123746E-7	45.000004	41
TGTCGAT	35	1.2123746E-7	45.000004	11
CGATAAT	70	0.0	45.000004	10
CCTTCGT	20	7.034206E-4	45.0	23
CAGGTCG	25	3.8915678E-5	45.0	45
CGGCGTA	20	7.034206E-4	45.0	19
AAGTACG	50	2.1827873E-11	45.0	1
AGCCGAA	40	6.8175723E-9	45.0	37
GCCCATA	40	6.8175723E-9	45.0	12
GCCTTCG	20	7.034206E-4	45.0	22
CGTATTC	40	6.8175723E-9	45.0	22
CGTATAT	20	7.034206E-4	45.0	23
GAATGTA	80	0.0	45.0	28
AGTCAAC	25	3.8915678E-5	45.0	23
>>END_MODULE