FastQCFastQC Report
Thu 26 May 2016
SRR1546789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546789_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3769376
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC213600.5666720433302489No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203800.5406730450875689No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG69860.18533571604424712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42450.11261810973487388No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC41550.1102304466309543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC207.035335E-445.00000429
ACTATCG406.8230293E-945.00000418
TCGTTAA302.1668238E-645.00000434
CACATCG302.1668238E-645.00000417
CGCGACT207.035335E-445.00000425
ATCGTCC302.1668238E-645.00000441
ACTAGTC207.035335E-445.00000417
CTAACCG800.045.0000041
CTAGTCC207.035335E-445.00000418
GTCCGCG207.035335E-445.00000415
ACTAAGA406.8230293E-945.0000049
CTACCGT302.1668238E-645.00000443
GTATCGA600.045.00000438
ACCACGT207.035335E-445.00000432
ATGCGCA600.045.00000420
ACCGTAG207.035335E-445.0000041
ACGTAAT302.1668238E-645.00000445
ATACGTG207.035335E-445.00000424
ATCGGCC302.1668238E-645.00000433
CACAACG406.8230293E-945.00000412