FastQCFastQC Report
Thu 26 May 2016
SRR1546788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546788_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3261624
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353601.0841225107492465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51100.15667041939843465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG406.8212103E-945.01
CTACGAT207.0351956E-445.028
TAGTCGA253.8923885E-545.01
CGTTTTT220600.043.0723041
CGGTCTA3000.042.031
CGTAAGG5150.041.067962
GCGATAA3350.040.2985089
TAATGCG1900.040.2631571
CTTAACG1600.039.3751
TACGATT403.4608274E-739.3753
ATTACGT356.251821E-638.57142625
GCGTAAG2600.038.0769271
GTTTTTT262300.038.017542
TAGGGTA13700.037.9379585
CGCATCG1850.037.702721
CCATACG301.14038194E-437.5000041
CGTACCA301.14038194E-437.50000424
TACGTTA301.14038194E-437.50000433
AGGGCGA20450.037.2982866
TCACGAC3450.037.17391225