##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546788_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3261624
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.630118922352793	31.0	31.0	33.0	30.0	34.0
2	32.008612274130925	33.0	31.0	34.0	30.0	34.0
3	32.009786842382816	33.0	31.0	34.0	30.0	34.0
4	35.751104971020574	37.0	35.0	37.0	35.0	37.0
5	35.71174114490205	37.0	35.0	37.0	33.0	37.0
6	35.767838352918666	37.0	35.0	37.0	33.0	37.0
7	36.106580954763636	37.0	35.0	37.0	35.0	37.0
8	35.9983946647437	37.0	35.0	37.0	35.0	37.0
9	37.775301506243515	39.0	38.0	39.0	35.0	39.0
10	37.43510901317871	39.0	37.0	39.0	35.0	39.0
11	37.14271142228534	39.0	37.0	39.0	34.0	39.0
12	36.416423536250655	38.0	35.0	39.0	33.0	39.0
13	36.17310303088278	38.0	35.0	39.0	32.0	39.0
14	37.1769762547737	39.0	35.0	41.0	32.0	41.0
15	37.39165765275212	39.0	35.0	41.0	33.0	41.0
16	37.49237373774537	39.0	35.0	41.0	33.0	41.0
17	37.40127004216305	39.0	35.0	41.0	33.0	41.0
18	37.250244050203214	39.0	35.0	41.0	33.0	41.0
19	37.147644241028395	38.0	35.0	41.0	33.0	41.0
20	36.97660551921374	38.0	35.0	41.0	32.0	41.0
21	36.82569664682379	38.0	35.0	41.0	32.0	41.0
22	36.76629985553209	38.0	35.0	41.0	32.0	41.0
23	36.66903573189307	38.0	35.0	41.0	32.0	41.0
24	36.62650323887732	38.0	35.0	40.0	32.0	41.0
25	36.524144107352654	38.0	35.0	40.0	32.0	41.0
26	36.47263694404996	38.0	35.0	40.0	32.0	41.0
27	36.370052771257505	38.0	35.0	40.0	31.0	41.0
28	36.369351893412606	38.0	35.0	40.0	31.0	41.0
29	36.28842012445334	37.0	35.0	40.0	31.0	41.0
30	36.25267811372494	37.0	35.0	40.0	31.0	41.0
31	36.05527645124024	37.0	35.0	40.0	31.0	41.0
32	35.779010701417455	37.0	35.0	40.0	30.0	41.0
33	35.58231114316059	37.0	35.0	40.0	29.0	41.0
34	35.30009712952811	37.0	35.0	40.0	27.0	41.0
35	35.105744868200624	37.0	34.0	40.0	27.0	41.0
36	34.96097894791061	37.0	34.0	40.0	25.0	41.0
37	34.65307374485839	37.0	34.0	40.0	23.0	41.0
38	34.73497037058839	37.0	34.0	40.0	24.0	41.0
39	34.6300514712916	37.0	34.0	40.0	24.0	41.0
40	34.49374452726617	36.0	34.0	40.0	23.0	41.0
41	34.43681031289934	36.0	34.0	40.0	23.0	41.0
42	34.427329759653475	36.0	34.0	40.0	23.0	41.0
43	34.30440510616797	36.0	34.0	40.0	23.0	41.0
44	34.17690144541492	36.0	34.0	40.0	23.0	41.0
45	34.15267639678884	35.0	34.0	40.0	23.0	41.0
46	33.9888022040554	35.0	34.0	40.0	23.0	41.0
47	33.87701862630395	35.0	33.0	40.0	23.0	41.0
48	33.74205426499192	35.0	33.0	40.0	22.0	41.0
49	33.800833879073735	35.0	33.0	40.0	23.0	41.0
50	33.668470062766275	35.0	33.0	40.0	22.0	41.0
51	33.07956097943846	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	6.0
11	9.0
12	9.0
13	36.0
14	41.0
15	106.0
16	223.0
17	570.0
18	1394.0
19	2848.0
20	5115.0
21	8365.0
22	13185.0
23	19447.0
24	29418.0
25	44339.0
26	60555.0
27	67315.0
28	66535.0
29	68180.0
30	75795.0
31	89732.0
32	112205.0
33	148599.0
34	273830.0
35	488592.0
36	219265.0
37	274396.0
38	416792.0
39	774529.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.91659247049936	23.05685756543366	29.52587422707216	16.500675736994822
2	33.08557332175628	24.5861570800313	29.38848867925917	12.939780918953259
3	27.711808595963237	23.779411728635797	35.64052754088147	12.868252134519492
4	24.95493042729634	26.558886002801056	34.18024272570965	14.305940844192955
5	22.588317966755213	30.420551234599696	32.67197567837371	14.319155120271374
6	20.83032869515309	38.38532583768086	30.222521050863005	10.561824416303043
7	83.75011957233575	4.090968180268479	10.350150722462184	1.8087615249335913
8	85.09303954103845	3.373442187082263	9.61398370872915	1.9195345631501364
9	81.00829525414333	5.0512566745891005	11.286003536888373	2.6544445343791927
10	48.04532956588497	24.27376668800573	17.956177658736873	9.724726087372426
11	41.70370343117417	21.559965219780086	23.83064387556628	12.90568747347947
12	36.20871075268026	21.74637542524828	27.4319480111748	14.612965810896657
13	21.924967439533187	35.10361709381584	27.531775581734742	15.439639884916225
14	16.06245845627822	37.35464296313738	31.29293873236155	15.289959848222848
15	15.595390517116629	24.046548590518096	45.60142432113573	14.756636571229548
16	18.438176810079888	20.80221386646652	43.40748657723882	17.352122746214768
17	19.543239809371038	20.496875176292544	30.461205828752792	29.498679185583626
18	22.324737615371973	23.518805355859534	34.41074139753693	19.74571563123156
19	27.31725054757998	25.109485336139297	28.0719972627133	19.501266853567426
20	29.152992496989228	24.566749570152783	27.572614133327445	18.70764379953054
21	23.64751424443774	25.636952634638448	31.089880378608935	19.625652742314873
22	23.571110587854392	22.936365442491223	30.142622202927132	23.34990176672725
23	20.57720325825417	27.828590910540267	28.552463435392923	23.04174239581264
24	20.759842336210426	24.06138782397971	37.108630547236594	18.07013929257327
25	19.165299249698922	25.28816932914401	35.55780801220496	19.988723408952104
26	18.07584197320108	32.49740006818689	29.70409832647785	19.722659632134175
27	18.775922669197918	30.73422932870251	31.92581364375538	18.564034358344188
28	16.809816214254003	27.433021096239173	37.841271710043834	17.915890979462993
29	17.143698967140296	24.907714684463937	37.91319906893008	20.035387279465688
30	19.654656698626205	28.09658624047407	33.84335533464311	18.405401726256613
31	27.1249230444711	25.758395204352187	28.052589752834784	19.06409199834193
32	27.56945619728086	26.845828949014354	28.51229939441211	17.07241545929267
33	26.083570638430427	27.575373494921546	27.745074232958792	18.595981633689227
34	20.217750421262537	28.420075398022583	29.923222296622786	21.4389518840921
35	20.287316993007167	27.575373494921546	31.413277557437645	20.72403195463364
36	27.738390445986415	25.605955806064706	28.074266071135117	18.58138767681376
37	21.761153339563357	29.33455848988111	29.208792920336617	19.695495250218908
38	21.479606478245195	31.507003872917295	25.496255852912537	21.517133795924977
39	21.408568246983712	29.613744564057658	28.23440102231281	20.74328616664582
40	23.157420965752028	27.1395783204931	26.314866459162673	23.38813425459219
41	19.86764875411758	25.685793334854047	28.07460332644106	26.37195458458731
42	22.98830275960687	26.817622141607984	25.845866966885207	24.348208131899938
43	22.912236358329473	27.274327145005067	26.69958278452697	23.113853712138493
44	20.98341194447919	30.391056725116076	27.668547938082376	20.956983392322353
45	19.121026825900227	34.137564599720875	24.86773460092273	21.873673973456167
46	22.619130837889344	31.263076307998716	26.86652416097012	19.25126869314182
47	22.46009963134929	27.619216684694496	27.820098208745094	22.10058547521112
48	23.772329367210933	25.71194595085148	29.292462895784432	21.223261786153156
49	21.979020267204312	24.745341584437693	31.065138102981827	22.21050004537617
50	20.060497469972013	30.9755814894666	27.78474772076732	21.179173319794064
51	19.80314101196214	32.90305688209309	25.451738152527696	21.84206395341707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2742.0
1	4038.0
2	5334.0
3	29512.5
4	53691.0
5	37533.5
6	21376.0
7	22163.5
8	22951.0
9	24416.5
10	25882.0
11	26322.0
12	26762.0
13	26552.5
14	26343.0
15	25192.5
16	24042.0
17	22324.5
18	20607.0
19	19865.5
20	19124.0
21	18870.5
22	18617.0
23	19396.5
24	20176.0
25	21326.5
26	24958.5
27	27440.0
28	30355.5
29	33271.0
30	38680.0
31	44089.0
32	50215.0
33	56341.0
34	68535.0
35	80729.0
36	86725.5
37	92722.0
38	112667.5
39	132613.0
40	170995.0
41	209377.0
42	248774.0
43	288171.0
44	293601.5
45	299032.0
46	287346.5
47	275661.0
48	264839.5
49	254018.0
50	239747.0
51	225476.0
52	204198.0
53	182920.0
54	169155.5
55	155391.0
56	147819.5
57	140248.0
58	127824.0
59	115400.0
60	104601.5
61	93803.0
62	85070.0
63	76337.0
64	66287.0
65	56237.0
66	47521.5
67	38806.0
68	32828.5
69	26851.0
70	22280.0
71	17709.0
72	15612.5
73	13516.0
74	10600.5
75	5709.0
76	3733.0
77	2927.0
78	2121.0
79	1639.0
80	1157.0
81	723.0
82	289.0
83	237.0
84	185.0
85	127.0
86	69.0
87	50.0
88	31.0
89	26.5
90	22.0
91	32.5
92	43.0
93	23.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3261624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.897278699210975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.89453756187204	16.2947843781157
2	7.167331536052386	2.8522078620495646
3	2.3846358455596817	1.4234329204568885
4	1.1832808784215358	0.9417627766960193
5	0.709374644576687	0.7057312502648037
6	0.5278282693725955	0.6301407708617243
7	0.39343886150251545	0.5479853874891073
8	0.30489064668388366	0.4853195335881519
9	0.2599701695645002	0.4655429025575396
>10	2.75079725020838	12.747862656471174
>50	1.0357451206703299	15.14963532547579
>100	1.3822322334186277	45.68993946908792
>500	0.005155800242086594	0.6771634629645779
>1k	4.687091129169631E-4	0.1304090679491532
>5k	1.5623637097232103E-4	0.15885347728728794
>10k+	1.5623637097232103E-4	1.0992287586846383
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35360	1.0841225107492465	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5110	0.15667041939843465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022565445925097436	0.0
2	0.0	0.0	0.0	0.06932129515848547	0.0
3	0.0	0.0	0.0	0.10792169790263992	0.0
4	0.0	0.0	0.0	0.17230680176501031	0.0
5	0.0	0.0	0.0	0.31502711532659805	0.0
6	0.0	0.0	0.0	0.5275899367922238	0.0
7	0.0	0.0	0.0	0.6869890582114921	0.0
8	0.0	0.0	0.0	1.0232632578126724	0.0
9	0.0	0.0	0.0	1.194159719207364	0.0
10	0.0	0.0	0.0	1.4266206037237892	0.0
11	0.0	0.0	0.0	1.6321317233378219	0.0
12	0.0	0.0	0.0	1.8084549292009133	0.0
13	0.0	0.0	0.0	1.8897027983605712	0.0
14	0.0	0.0	0.0	1.9226618396234514	0.0
15	0.0	0.0	0.0	1.9638069869488328	0.0
16	0.0	0.0	0.0	2.0406092179846604	0.0
17	0.0	0.0	0.0	2.135837852554433	0.0
18	0.0	0.0	0.0	2.271169208958482	0.0
19	0.0	0.0	0.0	2.3480020995675774	0.0
20	0.0	0.0	0.0	2.4305989899510183	0.0
21	0.0	0.0	0.0	2.539593772918031	0.0
22	0.0	0.0	0.0	2.654996406698013	0.0
23	0.0	0.0	0.0	2.7778186572088015	0.0
24	0.0	0.0	0.0	2.8775235894756723	0.0
25	0.0	0.0	0.0	2.961040267057147	0.0
26	0.0	0.0	0.0	3.047684221111937	0.0
27	0.0	0.0	0.0	3.132580579490462	0.0
28	0.0	0.0	0.0	3.221799937699747	0.0
29	0.0	0.0	0.0	3.325950508090448	0.0
30	0.0	0.0	0.0	3.4660647579242734	0.0
31	0.0	0.0	0.0	3.583031029940913	0.0
32	0.0	0.0	0.0	3.689297110887092	0.0
33	0.0	0.0	0.0	3.8067539360760163	0.0
34	0.0	0.0	0.0	3.9240574633986016	0.0
35	0.0	0.0	0.0	4.068739989649328	0.0
36	0.0	0.0	0.0	4.1986139420117095	0.0
37	3.065957326779543E-5	0.0	0.0	4.3272308518701115	0.0
38	3.065957326779543E-5	0.0	0.0	4.474795378008011	0.0
39	3.065957326779543E-5	0.0	0.0	4.670863349055563	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	40	6.8212103E-9	45.0	1
CTACGAT	20	7.0351956E-4	45.0	28
TAGTCGA	25	3.8923885E-5	45.0	1
CGTTTTT	22060	0.0	43.072304	1
CGGTCTA	300	0.0	42.0	31
CGTAAGG	515	0.0	41.06796	2
GCGATAA	335	0.0	40.298508	9
TAATGCG	190	0.0	40.263157	1
CTTAACG	160	0.0	39.375	1
TACGATT	40	3.4608274E-7	39.375	3
ATTACGT	35	6.251821E-6	38.571426	25
GCGTAAG	260	0.0	38.076927	1
GTTTTTT	26230	0.0	38.01754	2
TAGGGTA	1370	0.0	37.937958	5
CGCATCG	185	0.0	37.7027	21
CCATACG	30	1.14038194E-4	37.500004	1
CGTACCA	30	1.14038194E-4	37.500004	24
TACGTTA	30	1.14038194E-4	37.500004	33
AGGGCGA	2045	0.0	37.298286	6
TCACGAC	345	0.0	37.173912	25
>>END_MODULE