FastQCFastQC Report
Thu 26 May 2016
SRR1546787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546787_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2792670
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351981.2603708995334215No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63000.2255905638689856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG406.8212103E-945.0000041
TATATCG207.035021E-445.00000432
ACTACGC551.8189894E-1245.00000420
ATACGAT302.166622E-645.00000426
CGCGAAC253.892245E-544.99999627
CCCGTCG253.892245E-544.99999618
GTCGTAA253.892245E-544.99999632
CGTTTTT215200.044.0903781
GCGATAC1050.042.8571439
CTAACGG2050.041.7073172
TAACGGG9850.041.345183
TTACGCG556.184564E-1140.9090921
TCGACAC556.184564E-1140.90909234
ACGGTCT4200.040.71428730
TGTAGCG1500.040.5000041
TACTCGC451.9299478E-840.045
TCGTAAG1350.040.01
TATCGTG451.9299478E-840.01
TAGGGAC17100.039.4736865
TAGGGCG10500.039.2142875
Produced by FastQC (version 0.11.5)