##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546787_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2792670
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.581375529511185	31.0	31.0	33.0	30.0	34.0
2	31.951394185492735	31.0	31.0	34.0	30.0	34.0
3	31.93544600686798	33.0	31.0	34.0	30.0	34.0
4	35.69349726247641	37.0	35.0	37.0	33.0	37.0
5	35.64141126592114	37.0	35.0	37.0	33.0	37.0
6	35.69993876827552	37.0	35.0	37.0	33.0	37.0
7	36.05354553169548	37.0	35.0	37.0	35.0	37.0
8	35.94516358896683	37.0	35.0	37.0	35.0	37.0
9	37.73121027547114	39.0	38.0	39.0	35.0	39.0
10	37.38841502934468	39.0	37.0	39.0	34.0	39.0
11	37.0744284143848	39.0	37.0	39.0	33.0	39.0
12	36.223611454271364	38.0	35.0	39.0	32.0	39.0
13	35.930530281057194	38.0	35.0	39.0	32.0	39.0
14	36.92313950448854	39.0	35.0	41.0	32.0	41.0
15	37.15933568950145	39.0	35.0	41.0	32.0	41.0
16	37.277574865630385	39.0	35.0	41.0	33.0	41.0
17	37.178536311128774	38.0	35.0	41.0	33.0	41.0
18	37.034673627746926	38.0	35.0	41.0	32.0	41.0
19	36.90893481865025	37.0	35.0	41.0	32.0	41.0
20	36.696745408515866	37.0	35.0	41.0	32.0	41.0
21	36.53675765486076	37.0	35.0	40.0	32.0	41.0
22	36.47223624703241	37.0	35.0	40.0	32.0	41.0
23	36.40263690303544	37.0	35.0	40.0	32.0	41.0
24	36.331430852911375	37.0	35.0	40.0	32.0	41.0
25	36.22616743116802	37.0	35.0	40.0	31.0	41.0
26	36.15053443478821	37.0	35.0	40.0	31.0	41.0
27	36.048445752631	36.0	35.0	40.0	31.0	41.0
28	36.05778018885153	36.0	35.0	40.0	31.0	41.0
29	35.97032230804212	36.0	35.0	40.0	31.0	41.0
30	35.94238237958656	36.0	35.0	40.0	31.0	41.0
31	35.713814736435026	36.0	35.0	40.0	30.0	41.0
32	35.391253173486305	36.0	34.0	40.0	29.0	41.0
33	35.17596422062041	36.0	34.0	40.0	28.0	41.0
34	34.88701529360791	36.0	34.0	40.0	26.0	41.0
35	34.66831240354213	36.0	34.0	40.0	24.0	41.0
36	34.4849767426871	36.0	34.0	40.0	23.0	41.0
37	34.15254899433159	36.0	33.0	40.0	23.0	41.0
38	34.25731396835287	35.0	34.0	40.0	23.0	41.0
39	34.16432589600633	36.0	34.0	40.0	23.0	41.0
40	34.01471996333258	36.0	33.0	40.0	22.0	41.0
41	33.97550910060981	36.0	34.0	40.0	22.0	41.0
42	33.97767047305983	35.0	33.0	40.0	22.0	41.0
43	33.851066183974474	35.0	33.0	40.0	22.0	41.0
44	33.724324392069235	35.0	33.0	40.0	21.0	41.0
45	33.70385330167904	35.0	33.0	40.0	22.0	41.0
46	33.53438537313753	35.0	33.0	40.0	20.0	41.0
47	33.429381559582765	35.0	33.0	40.0	20.0	41.0
48	33.297690382322294	35.0	33.0	40.0	20.0	41.0
49	33.3603737641755	35.0	33.0	39.0	20.0	41.0
50	33.20505072206885	35.0	33.0	39.0	20.0	41.0
51	32.62358567249263	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	5.0
13	13.0
14	30.0
15	86.0
16	218.0
17	609.0
18	1478.0
19	3091.0
20	5349.0
21	8844.0
22	13467.0
23	19850.0
24	29207.0
25	43448.0
26	58905.0
27	63729.0
28	62212.0
29	62398.0
30	68618.0
31	80504.0
32	100050.0
33	131867.0
34	248232.0
35	462710.0
36	176593.0
37	216719.0
38	324397.0
39	609797.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.24505938761114	23.407563371254035	29.42080517927288	17.926572061861947
2	33.1108580677273	25.224462611049642	29.147948021069443	12.516731300153616
3	27.1906455112849	23.74326361510669	35.84358338077897	13.222507492829443
4	24.418388137517145	26.101257935953765	35.640730913426935	13.839623013102157
5	22.84254852882725	30.38543759198187	33.85147546971179	12.920538409479102
6	20.448961030125293	38.13912850426295	30.582560775172148	10.829349690439615
7	82.45478341515468	4.904124010355681	10.860538481095153	1.7805540933944934
8	84.23716371787573	3.8162761801430176	10.360407781800212	1.5861523201810452
9	80.66925200614466	5.381767269315745	11.413987331120397	2.534993393419201
10	51.064894885539644	21.732714570643864	17.61658198068515	9.58580856313134
11	45.407907128303734	20.303866908728924	21.90853197835763	12.37969398460971
12	39.27438616091411	21.378931273655677	25.664901330984325	13.68178123444589
13	20.93054317194656	39.11518367726942	25.886696244096154	14.067576906687865
14	14.926396602534492	40.68404072088718	30.108462510787167	14.28110016579116
15	14.03005009542839	23.30801705894359	48.77912535315666	13.882807492471363
16	15.328162654377353	19.65054947415914	47.26559170972582	17.755696161737692
17	16.37046267550409	20.090952386067812	30.819144403026495	32.7194405354016
18	20.953890004905702	24.918411412734052	34.45623722101072	19.671461361349532
19	27.90970648161079	26.098894606237046	27.105923721742993	18.885475190409178
20	29.69008153487523	24.59767892375397	27.320843493860714	18.39139604751009
21	21.8387779436883	27.741802647645443	30.247290227631623	20.172129181034638
22	21.310502135948752	25.656987757235903	28.465554469378766	24.566955637436575
23	19.472511968832695	30.00519216377159	27.70166185048717	22.82063401690855
24	19.973072364439766	24.983367171917912	38.25675786970892	16.786802593933402
25	17.367250695570906	26.622049866257026	35.98459538721009	20.026104050961983
26	16.571560549581584	34.60057937386086	29.468358237815423	19.359501838742137
27	18.301768558404678	32.50538015590815	31.97259253689122	17.220258748795956
28	15.43404698729173	29.411495092510037	38.80272284229787	16.35173507790036
29	15.974067827562871	26.536110603830743	38.20719956170976	19.282622006896627
30	18.404143704769986	29.42990041787966	34.10761027976811	18.058345597582242
31	28.00749820064669	26.674974128701205	26.845205484357265	18.472322186294836
32	28.36636623732843	27.61264309782395	27.82240651419609	16.198584150651527
33	25.64266454683153	28.43694385659602	26.705912263174667	19.214479333397787
34	18.716139035403394	29.529052841903987	29.729506171513282	22.025301951179337
35	20.054499815588667	27.138795489621042	31.27644870321234	21.530255991577953
36	27.981608997840773	26.986862035256582	27.16085323364379	17.870675733258853
37	20.403842917351493	32.72799865361822	29.5327052605571	17.33545316847318
38	20.827881561373164	33.61027260650202	24.53061049103546	21.031235341089353
39	21.338754668471392	32.00614465726348	27.20958795704469	19.44551271722044
40	23.311168165232555	27.657617978493697	26.018648819946506	23.012565036327242
41	18.076929963081927	26.19274744241174	27.87909061937143	27.851231975134905
42	22.313986256879616	28.067870532501153	24.72576423279514	24.89237897782409
43	22.614666251293563	28.061460895845197	25.87144202501549	23.452430827845753
44	20.11082584050389	32.4823555951831	27.12013950806934	20.286679056243667
45	17.55520702410238	37.052426530882634	23.299852829013094	22.09251361600189
46	21.807696577110793	33.624774857036456	25.814936959970208	18.752591605882543
47	22.134086734200604	28.801004057049344	27.350349307293737	21.71455990145631
48	23.90311064321957	25.10192754604017	29.680234327722214	21.314727483018046
49	20.786773947512597	25.951472963149964	30.794042976792817	22.467710112544626
50	19.501588085953586	33.11991749830807	27.15458682909187	20.22390758664647
51	18.52857659515804	33.881196131300875	24.977279807496053	22.61294746604504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2883.0
1	3670.5
2	4458.0
3	27551.0
4	50644.0
5	35191.5
6	19739.0
7	20705.0
8	21671.0
9	23345.5
10	25020.0
11	26013.0
12	27006.0
13	26823.5
14	26641.0
15	25721.5
16	24802.0
17	23064.5
18	21327.0
19	20397.5
20	19468.0
21	17981.0
22	16494.0
23	16572.0
24	16650.0
25	18186.0
26	21814.5
27	23907.0
28	27663.5
29	31420.0
30	36430.0
31	41440.0
32	47233.0
33	53026.0
34	55847.0
35	58668.0
36	67174.5
37	75681.0
38	92528.0
39	109375.0
40	146700.5
41	184026.0
42	224285.0
43	264544.0
44	263640.0
45	262736.0
46	255822.5
47	248909.0
48	230476.5
49	212044.0
50	203480.0
51	194916.0
52	178076.0
53	161236.0
54	143060.5
55	124885.0
56	119618.5
57	114352.0
58	102917.5
59	91483.0
60	83888.5
61	76294.0
62	66850.0
63	57406.0
64	46293.0
65	35180.0
66	29956.0
67	24732.0
68	20906.5
69	17081.0
70	14888.5
71	12696.0
72	11194.0
73	9692.0
74	7418.5
75	3878.0
76	2611.0
77	2110.0
78	1609.0
79	1147.5
80	686.0
81	466.0
82	246.0
83	157.0
84	68.0
85	44.5
86	21.0
87	21.0
88	21.0
89	12.0
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2792670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.999672437909105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04780365682845	17.229770010432784
2	6.859801535330477	2.8810717046201195
3	2.382036125792812	1.5006593513074529
4	1.2319219356762856	1.034798284731077
5	0.8027342005916451	0.8428577633565684
6	0.5723531598736983	0.7211537325689932
7	0.41891003650171676	0.6157881483242019
8	0.3278380456854755	0.5507593257663411
9	0.2797569038480527	0.5287323008747464
>10	2.822466939655406	13.726328407722676
>50	1.026729491552832	15.820305158418984
>100	1.2184838466717642	41.72346686325223
>500	0.007953766256788952	1.0308075896080455
>1k	8.645398105205382E-4	0.2867045942986765
>5k	1.7290796210410765E-4	0.22875366566383762
>10k+	1.7290796210410765E-4	1.2780430990532947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35198	1.2603708995334215	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6300	0.2255905638689856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5808026010950094E-5	0.0	0.0	0.019372142071924	0.0
2	3.5808026010950094E-5	0.0	0.0	0.06638808022430147	0.0
3	3.5808026010950094E-5	0.0	0.0	0.10660049343459843	0.0
4	3.5808026010950094E-5	0.0	0.0	0.1719143328785714	0.0
5	3.5808026010950094E-5	0.0	0.0	0.3251726842054378	0.0
6	3.5808026010950094E-5	0.0	0.0	0.5658384270250334	0.0
7	3.5808026010950094E-5	0.0	0.0	0.7546541481807733	0.0
8	3.5808026010950094E-5	0.0	0.0	1.1073990124146427	0.0
9	3.5808026010950094E-5	0.0	0.0	1.3056322444112622	0.0
10	3.5808026010950094E-5	0.0	0.0	1.5572194351641977	0.0
11	3.5808026010950094E-5	0.0	0.0	1.7713514307096794	0.0
12	3.5808026010950094E-5	0.0	0.0	1.9624946735561308	0.0
13	3.5808026010950094E-5	0.0	0.0	2.049937873074871	0.0
14	3.5808026010950094E-5	0.0	0.0	2.092048111663748	0.0
15	3.5808026010950094E-5	0.0	0.0	2.128894570429016	0.0
16	3.5808026010950094E-5	0.0	0.0	2.199221533514522	0.0
17	3.5808026010950094E-5	0.0	0.0	2.284086555160474	0.0
18	3.5808026010950094E-5	0.0	0.0	2.4120286320975985	3.5808026010950094E-5
19	3.5808026010950094E-5	0.0	0.0	2.481245546376765	3.5808026010950094E-5
20	3.5808026010950094E-5	0.0	0.0	2.5540074552310155	3.5808026010950094E-5
21	3.5808026010950094E-5	0.0	0.0	2.651262053876756	3.5808026010950094E-5
22	3.5808026010950094E-5	0.0	0.0	2.756072146010807	3.5808026010950094E-5
23	3.5808026010950094E-5	0.0	0.0	2.8752770646012595	3.5808026010950094E-5
24	3.5808026010950094E-5	0.0	0.0	2.9635080406922407	3.5808026010950094E-5
25	3.5808026010950094E-5	0.0	0.0	3.0362699495464915	3.5808026010950094E-5
26	3.5808026010950094E-5	0.0	0.0	3.105844944085767	3.5808026010950094E-5
27	3.5808026010950094E-5	0.0	0.0	3.1802182141105106	3.5808026010950094E-5
28	3.5808026010950094E-5	0.0	0.0	3.2612875849993017	3.5808026010950094E-5
29	3.5808026010950094E-5	0.0	0.0	3.352025122911049	3.5808026010950094E-5
30	3.5808026010950094E-5	0.0	0.0	3.4709077692674035	3.5808026010950094E-5
31	3.5808026010950094E-5	0.0	0.0	3.5806235609649546	3.5808026010950094E-5
32	7.161605202190019E-5	0.0	0.0	3.677269423168509	3.5808026010950094E-5
33	7.161605202190019E-5	0.0	0.0	3.7796803775598264	3.5808026010950094E-5
34	7.161605202190019E-5	0.0	0.0	3.88950359333541	3.5808026010950094E-5
35	7.161605202190019E-5	0.0	0.0	4.016335621466196	3.5808026010950094E-5
36	7.161605202190019E-5	0.0	0.0	4.1287012070885565	3.5808026010950094E-5
37	7.161605202190019E-5	0.0	0.0	4.245005675572123	3.5808026010950094E-5
38	1.0742407803285029E-4	0.0	0.0	4.360486559457437	3.5808026010950094E-5
39	1.0742407803285029E-4	0.0	0.0	4.487211163510189	3.5808026010950094E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	40	6.8212103E-9	45.000004	1
TATATCG	20	7.035021E-4	45.000004	32
ACTACGC	55	1.8189894E-12	45.000004	20
ATACGAT	30	2.166622E-6	45.000004	26
CGCGAAC	25	3.892245E-5	44.999996	27
CCCGTCG	25	3.892245E-5	44.999996	18
GTCGTAA	25	3.892245E-5	44.999996	32
CGTTTTT	21520	0.0	44.090378	1
GCGATAC	105	0.0	42.857143	9
CTAACGG	205	0.0	41.707317	2
TAACGGG	985	0.0	41.34518	3
TTACGCG	55	6.184564E-11	40.909092	1
TCGACAC	55	6.184564E-11	40.909092	34
ACGGTCT	420	0.0	40.714287	30
TGTAGCG	150	0.0	40.500004	1
TACTCGC	45	1.9299478E-8	40.0	45
TCGTAAG	135	0.0	40.0	1
TATCGTG	45	1.9299478E-8	40.0	1
TAGGGAC	1710	0.0	39.473686	5
TAGGGCG	1050	0.0	39.214287	5
>>END_MODULE