FastQCFastQC Report
Thu 26 May 2016
SRR1546786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546786_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2720159
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.4587599474883637No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78910.29009333645569985No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44740.16447567954667355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGA408.3200575E-944.02
CGTTTTT180200.043.096561
GAGCGAT16600.041.216877
CGACGGT4600.040.65217228
CGGTCTA4700.039.78723531
TATGCGA1500.039.62
AGCGATC1500.039.68
AGCGATA4200.039.2857138
GACGGTC4800.038.95833229
GTTAGCG2350.038.382981
CACGACG5000.038.2826
GACCGAT13400.038.0895549
GTTTTTA83100.037.990372
ACGGTCT4950.037.7777830
TACGGGT1750.037.7142874
CGAGATT4950.036.888896
ACGTGCG1200.036.6666641
ACGTACT301.301563E-436.66666419
CCGTTCG301.301563E-436.66666426
ACGAGAC3550.035.943665
Produced by FastQC (version 0.11.5)